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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Stbl3 one shot competent cells (Invitrogen, no. C737303) were then transformed with pEXPRESS and grown on ampicillin (100 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... one drop of Hoechst 33342 Ready Flow (Invitrogen) was added and cells were returned to the incubator for 1h at 37 °C ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and a Step-One PCR instrument (Applied Biosystems) were utilized for the qRT-PCR reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... coli One Shot BL21 Star (DE3) cells (Invitrogen). The unlabeled expression was carried out in the Terrific Broth medium (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... and one-part DMEM/F12 (Fisher Scientific; 11320033) supplemented with 5 μg/ml insulin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of HIV-1 YU2 or HIV-1 YU2 lacking Vpr (HIV-1 YU2 ΔVpr) and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). To make VSV-G pseudotyped HIV-1 GFP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
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bioRxiv - Microbiology 2024Quote: ... ten-fold serial dilutions from 10-1 to 10-6 of media from infections were prepared in 1 x PBS (Gibco, #14190250). The media on Vero cells seeded the previous day was aspirated ...
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bioRxiv - Microbiology 2022Quote: ... Mouse cytokine levels in vaccinated animals (IL-4, IL-10, IL-12, and IFN-γ) was measured by ELISA following the supplier’s protocol (Invitrogen or R&D Systems).
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded (1 x 105 cells/ml) on gelatinised 6 well plates (Nunc, Roskilde, Denmark) and grown to ~80 % confluency ...
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bioRxiv - Cell Biology 2022Quote: ... with 1 µl of the newly synthesized cDNA on the QuantStudio 6 Flex instrument (Applied Biosystems) in a 96 well format ...
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bioRxiv - Neuroscience 2021Quote: ... AB_2534017, 1:400), Alexa 488 (A21206 RRID: AB_141708 and custom, 1:400) and the nuclear stain DAPI (Invitrogen Molecular Probes D-21490, 1:5000) for visualization by epifluorescence microscopy.
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bioRxiv - Neuroscience 2021Quote: ... AB_2534017, 1:400), Alexa 488 (A21206 RRID: AB_141708 and custom, 1:400) and the nuclear stain DAPI (Invitrogen Molecular Probes D-21490, 1:5000) for visualization by epifluorescence microscopy.
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Live Cell Analysis System ...
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bioRxiv - Bioengineering 2019Quote: ... via covalent attachment to COOH groups on the particles via standard EDC chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo Fisher Scientific, MA) and N-hydroxysulfosuccinimide sodium salt (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16°C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3).
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 1 μm FluoSpheres (1 to 800 dilution, 585/608 nm, ThermoFisher Scientific, F13083), were applied to the grid in the humidity chamber of the Leica EM GP plunger prior to the vitrification step ...
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run using primer/probes at 1:20 dilution and Taqman FastVirus one-step reaction mix (Thermo Scientific), and amplified using an Applied Biosystems QS3 cycler ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed three times with PBS and incubated for one hour with secondary antibody (Alexa Fluor 594, chicken anti-mouse 1:1’000, Invitrogen) in PBS with 0.1 % Triton X-100 and 6 % FBS ...
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bioRxiv - Genomics 2021Quote: ... Samples were transferred to 1.5 mL safe-lock tubes containing one scoop of acid-washed glass beads (<106μM) and 1 mL TRIzol (Invitrogen) containing 20μg/mL GenElute-LPA (Sigma-Aldricht ...
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bioRxiv - Neuroscience 2022Quote: ... and one or more of the following antibodies: goat anti-rabbit IgG Alexa Fluor 568 (1:1000, #A11036, Invitrogen), goat anti-mouse IgG Alexa Fluor 647 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1%TBS-Tween for one hour and incubated with anti-Biotin (Invitrogen, Cat # 31852, RRID: AB_228243, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for one series included rabbit anti-beta Amyloid (1:300, Cat# 715800, Lot# SH257822; Invitrogen, Waltham, MA). Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500 ...
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bioRxiv - Immunology 2022Quote: ... After one washing step (1% BSA/PBS) cells were permeabilized in PermBuffer (saponin-based permeabilization and wash buffer; ThermoFisher) for 15min and subsequently stained with pAKT Ser473 (CellSignaling ...
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bioRxiv - Neuroscience 2020Quote: ... and AMG44 (final concentration: 5μM) treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018], 1% N2 [Gibco, 17502048] ...
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bioRxiv - Molecular Biology 2021Quote: Cells were lysed by adding one volume of TNES buffer (TNE + 1% SDS) supplemented with RNaseA (Invitrogen cat #12091021) at final concentration of 100 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and secondary antibody for one hour (Optogenetics experiment: Alexa Fluor 488 goat anti chicken, Invitrogen (cat# A11039), 1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for one hour at room temperature (1:1000, Alexa Fluor 488 Goat anti-Mouse IgG, Invitrogen A-11001), and similarly washed ...
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bioRxiv - Physiology 2023Quote: ... and one of the following secondary antibodies: Donkey anti-rabbit Alexa Fluor 488 (Invitrogen; Catalog# A21206; 1:250 dilution), Donkey anti-goat Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... USA) and incubated for one hour at room temperature with Streptavidin Alexa Fluor™ 568 conjugate (1:500)(Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular DNA was extracted with one volume of phenol:chloroform:isoamyl alcohol (25:24:1, #15593031, ThermoFisher Scientific, Waltham, MA, USA), precipitated with one volume of isopropyl alcohol ...
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... hTRESK and hTASK-3/hTASK-1 were subcloned into the pCDNA3 vector (Invitrogen). The cDNAs of hTREK-1 ...
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bioRxiv - Immunology 2021Quote: JEG-3 cells were infected with ZIKV for 1 h in DMEM (Gibco) with 2% FCS at an MOI of 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). The retinal and optic nerve slices were analyzed with a confocal laser-scanning microscope (LSM 510 META ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μl of sample on a 1 mm thick glass slide (Fisher Scientific) and an 18 x18 mm cover slip (VWR ...
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bioRxiv - Molecular Biology 2021Quote: ... siRNAs were transfected on day 1 and 3 with LipofectamineTM RNAiMAX (Thermo Fisher) and collected on day 8 for analysis ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then treated with hexamethyldisilazane-trimethylchlorosilane-pyridine solution (3:1:9; ThermoFisher) for 20 min at 110°C ...
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bioRxiv - Physiology 2020Quote: ... 1 h with oxygenated water 3% (Tyramide Superboost Kit, B40922, Thermo Fisher, USA) and ...