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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 10 dextrose (Fisher Scientific), 1.3 L-ascorbic acid (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted on Superfrost Plus microscope slides (Fisher Scientific, #12-550-15) using Fluoro-Gel with TES buffer (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... all from Invitrogen, were incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and run on the QuantStudio 3 system (Applied Biosystems, Waltham, Massachusetts). Human 18S rRNA was used as the housekeeping gene for normalization ...
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bioRxiv - Neuroscience 2024Quote: ... Afterwards slices were repeatedly washed and incubated overnight at RT in PBS containing a mixture of Alexa Fluor 647-conjugated streptavidin (1:500, S21374, Invitrogen, UK) and secondary antibodies (donkey anti-goat Alexa Fluor Plus 488 ...
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bioRxiv - Neuroscience 2024Quote: ... After 3×10 min washes in PBS slices were incubated for 2h at RT in PBS containing a secondary antibody (donkey anti-goat Alexa Fluor Plus 647, 1:500, A21447, Invitrogen, UK). During a final round of washes tissue slices were counterstained with DAPI (NucBlue™ Fixed Cell ReadyProbes™ Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA pellets were dissolved in RNase/DNase free ultrapure water and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). One microgram of RNA was converted into cDNA using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... prepared fresh from 16% methanol-free stock (28906, Thermo Fisher Scientific, Poland). Brains were extracted and postfixed overnight in PBS with 4% PFA at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... One microgram of RNA was converted into cDNA using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). qPCR was performed using PowerUp Sybr Green reagents (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin (10378016, Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... HeLa, U2OS, Neuro2a and MEFs (Fratta et al., 2018) were grown in DMEM media (31966, Gibco), whereas SH-SY5Y and SK-N-BE were grown in DMEM/F-12 (50:50) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 10 μg/mL of actinomycin D (Invitrogen) for 2 and 4 h ...
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bioRxiv - Neuroscience 2024Quote: ... and NER (100 μL) buffers according to manufacturer’s protocol of the NE-PER™ Nuclear and Cytoplasmic Extraction Reagent kit (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... while the HIF1α-specific siRNA was obtained from Ambion. The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion) ...
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bioRxiv - Neuroscience 2024Quote: ... All secondary Alexa Fluor conjugated antibodies were raised in goat and purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Neuroscience 2024Quote: ... The Protein A/G Magnetic Beads from Thermo Scientific Pierce (#88803 ...
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bioRxiv - Neuroscience 2024Quote: ... During a final round of washes tissue slices were counterstained with DAPI (NucBlue™ Fixed Cell ReadyProbes™ Reagent, R37606, Invitrogen, UK) according to the kit manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... All slices were stained with DAPI (Thermo Fisher, #D1306) and mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and iron chloride (FeCl2) were purchased from Fisher Scientific (Waltham, MA). The proteasome inhibitor MG-132 was purchased from Tocris Bioscience (Minneapolis ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was performed using PowerUp Sybr Green reagents (Applied Biosystems) and run on the QuantStudio 3 system (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected into U2OS or HeLa cells using Lipofectamine RNAiMAX reagents (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... anti mouse Alexa Fluor 488 (IF secondary 1:600, Thermo Fisher, Z25002); anti rabbit Alexa Fluor 488 (IF secondary 1:600 ...
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bioRxiv - Neuroscience 2024Quote: ... and secondary antibodies (donkey anti-goat Alexa Fluor Plus 488, 1:500, A32814, Invitrogen, UK). Following another round of washing ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then mounted on glass slides in Prolong Glass medium (P36980, Invitrogen, UK), coverslipped and cured.
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bioRxiv - Neuroscience 2024Quote: ... and SUPERase RNase inhibitor (Invitrogen, 1:100). Lysates were cleared twice through centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... all supplemented with 10% FBS (10082147, Invitrogen) and 1% penicillin/streptomycin (10378016 ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1ml Dounce Homogenizer using the loose pestle (Gibco, Cat#: A1247501). Homogenized brain lysate was then transferred to a 15ml Falcon tube (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Life technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (ThermoFisher)) freshly supplemented with 100 ng/mL IL-34 ...
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bioRxiv - Neuroscience 2024Quote: ... Lipofectamine 3000 (L3000015, Thermo Fisher) or electroporation with an Amaxa Nucleofector 2b device (AAB-1001 ...
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bioRxiv - Physiology 2024Quote: ... qPCR was performed using SYBR Green I (Thermo Fisher Scientific) in a CFX Connect system (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) and RNeasy Mini Kit (Qiagen ...
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bioRxiv - Physiology 2024Quote: ... (Thermo Fisher Scientific, Waltham, MA). The frozen blocks were sectioned at 12 μm thickness and mounted in phosphate-buffered saline (PBS).
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bioRxiv - Physiology 2024Quote: ... and polyadenylated mRNA was extracted using the Dynabeads mRNA DIRECT kit (Invitrogen) and reverse transcribed with the Reverse Transcriptase Core kit (Eurogentec) ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR products were then purified by PCR purification kit (Invitrogen) and used as template DNA to generate each sgRNA by in vitro transcription using MEGAshortscript T7 kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Neuroscience 2024Quote: Relative fold change of MARK3 was determined by qRT-PCR using SYBR Green Master Mix (ThermoFisher, A25776) with GAPDH as an endogenous control.
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bioRxiv - Neuroscience 2024Quote: ... using a Varioskan microplate reader (ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... Using mMESSAGE mMACHINE SP6 kit (Invitrogen), capped Cas9-mRNA was synthesized and then purified by ethanol precipitation as indicated in the manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... phospho-tau Ser262 (ThermoFisher, 44-750-G, 1:2000), TAU-5 (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... The extracted DNA was checked using the Qubit dsDNA BR assay (Invitrogen, Q32850) to ensure proper extraction occurred ...
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bioRxiv - Neuroscience 2024Quote: ... + L-Glutamine (GIbco; 2 mM) + Penicillin-Streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 5% KnockOut Serum Replacement (Thermo Fisher Scientific), 1 mM glutamine (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM glutamine (Invitrogen), 1% nonessential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and 4 ng/ml fibroblast growth factor (FGF) (Thermo Fisher Scientific/Peprotech), 1×Penicillin-Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... or a PureLink Quick Plasmid Miniprep Kit (Invitrogen). We performed Sanger sequencing on multiple stocks of the BFP control plasmids ...
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bioRxiv - Neuroscience 2024Quote: ... + Penicillin-Streptomycin (Gibco; 100U/ml) + SHH C25II (R&D systems ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were passaged as aggregates every 5-7 days using a collagenase IV solution (Gibco) to detach hESC colonies ...
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bioRxiv - Neuroscience 2024Quote: ... 1% nonessential amino acids (Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma ...