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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... HMLE.M cells were induced after prolonged treatment with 2.5 ng/ml recombinant TGFβ1 (Life Technologies, Cergy-Pontoise, France). Cell proliferation and survival analyses were performed in 3 or more independent experiments ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Synthetic Biology 2019Quote: ... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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bioRxiv - Immunology 2020Quote: ... Recombinant viruses were generated in Vero E6 cells (ATCC-CRL1586) grown in DMEM high glucose media (Gibco #11995065) supplemented with 10% Hyclone Fetal Clone II (GE #SH3006603HI) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant baculoviruses were produced using the Bac-to-Bac baculovirus expression system according to the manufacturer’s manual (Invitrogen).The scheme for constructing the recombinant baculoviruses ...
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bioRxiv - Microbiology 2022Quote: ... Bacmids and recombinant baculoviruses were generated from the pFastBac Dual vectors using the Bac-to-Bac system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB10 with DNA isolated from recombinant strains (via the Whole Blood Genomic DNA Purification Kit, Thermo Scientific) followed by selection on LB agar with the appropriate drug ...
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and the concentration of the recombinant plasmids was determined using the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). 293FT cells were transfected with 1 µg of recombinant plasmids or blank vectors with 40 ng of pRL-TK Renilla luciferase control vector using Lipofiter ...
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bioRxiv - Genomics 2023Quote: ... residual RNA contamination was removed by incubating the samples in 10µL of 1mg/mL recombinant RNase (Ambion, #AM2269). Sample aliquots of 100µL were kept at -80 °C.
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B], 3.33 mM each dNTP [ThermoFisher, R1121] ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Genetics 2024Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was in vitro transcribed from the linearized recombinant infectious clone using the mMESSAGE mMACHINE Kit (Ambion) and electroporated into Vero E6 cells ...
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488) or anti-rabbit antibody (Thermofisher, Goat anti-Rabbit IgG (H+L) Secondary Antibody ...
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bioRxiv - Physiology 2019Quote: ... anti-rabbit (Alexa Fluor® 488 Donkey anti-rabbit, Thermo Fisher A21206; 1:1000) and DAPI (Hoechst 33342 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-Rabbit Alexa 488 and anti-Rabbit Cy3 (1:200) procured from Molecular probes, USA.
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594; 1:2000; Invitrogen). Duolink Mounting Medium with DAPI was used to mount the coverslips ...
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bioRxiv - Developmental Biology 2023Quote: ... pH 7.6 (Rabbit anti-HA, Cell Signaling, 37245; rabbit anti-Podocin, Invitrogen, PA5-79757). Sections were incubated overnight at 4°C followed by 4×10 min washes in TBS/BSA-Ac to remove unbound antibody ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
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bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates (3μg/μl in 1xSDS ...