Labshake search
Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: 20µg of rhEPO and reference product Eprex® were treated with 20 units of recombinant PNGase F (Thermo Fisher Scientific, USA) at 37 ºC for hours ...
-
bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
-
bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
-
bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
-
bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
-
bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
-
bioRxiv - Cancer Biology 2023Quote: ... LN single cell suspension were stimulated for 5h at 37°C with 5ug/ml of LCMV-GP61-80 in the presence of 50 U/ml recombinant murine IL2 (Thermofisher P2747) and 1mg/ml Brefeldin A (Sigma) ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
-
bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
-
bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
-
bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
-
bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
-
bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
-
Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
-
bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
-
bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were detected with goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 rabbit (Thermo Fisher Scientific Cat# A32731TR ...
-
bioRxiv - Neuroscience 2020Quote: ... the primary antibody rabbit anti-GFP (Sysy 132002, RRID:AB_887725) and the secondary antibody AlexaFluor 488 goat anti-rabbit (Invitrogen).
-
bioRxiv - Neuroscience 2020Quote: ... and a secondary anti-rabbit antibody (donkey anti-rabbit Alexa Fluor 488 (1:1000, #A21206, Thermo Fisher Scientific). Images were acquired with a fluorescent microscope (BZX800 ...
-
bioRxiv - Molecular Biology 2022Quote: ... rabbit monoclonal anti-LIMP2 (SCARB2; #702770) and rabbit polyclonal anti-FASN (#PA5-22061) antibodies were purchased from ThermoFisher. Mouse monoclonal anti-IRS4 (#sc-100854 ...
-
bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 568 anti-rabbit IgG (A11036) and Alexa Fluor 647 anti-rabbit IgG (A21245) from Thermo Fisher.
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
-
bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit COL5A1 (1:500; #PA5-83158) and anti-rabbit SIX3 (1:200; #PA5-103901) from Thermo Fisher; anti-mouse COL3A1 (1:300 ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary goat anti-rabbit Alexa Fluor 546 or goat anti-rabbit Alexa Fluor 488 antibodies (1:1000; Invitrogen) were incubated for one hour ...
-
bioRxiv - Neuroscience 2022Quote: ... and a secondary anti-rabbit antibody (donkey anti-rabbit Alexa Fluor 488 (1:1000, #A21206, Thermo Fisher Scientific). Images were acquired with a fluorescent microscope (BZX800 ...
-
bioRxiv - Microbiology 2023Quote: ... and rabbit anti-lysozyme (1:500, Novus) with goat anti-rabbit IgG Alexa Fluor 568 (Thermo Fisher Scientific); rabbit anti-villin (1:1000 ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were incubated with an anti-rabbit secondary antibody (1:300 AlexaFluor 594nm goat anti-rabbit, Invitrogen A11037), nuclei were stained with Hoechst 33342 (Invitrogen™ R37605 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rabbit anti-SDHC or rabbit anti-SDHD antibody (1:200, AB_2634976 and 1:200, AB_2717499, Fisher Scientific, Germany) as well as mouse anti-CD4 monoclonal antibody 4B12 (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Alexa-594 donkey anti-rabbit/anti-mouse or Alexa-700 donkey anti-rabbit secondary antibodies (ThermoFisher Scientific, UK) and DAPI (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2024Quote: ... Alexa 488-conjugated anti-rabbit IgG and Alexa 633-conjugated anti-rabbit IgG (all from Thermo Fisher Scientific). Mitochondria were stained with MitoTracker Orange CMXRos (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were incubated with an anti-rabbit secondary antibody (1:300 AlexaFluor 594nm goat anti-rabbit, Invitrogen A11037), nuclei were stained with Hoechst 33342 (Invitrogen™ R37605 ...
-
bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-pERK 1:500 (Invitrogen, 700012), rabbit anti-AKT 1:1,000 (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Rab11 (1:50, Thermo Fisher Scientific Cat# 71-5300 ...
-
bioRxiv - Cell Biology 2021Quote: ... or rabbit IgG (Fisher Scientific, Hampton, NH) was added and mixed by rotation overnight at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... or rabbit IgG (Fisher Scientific, Hampton, NH) was added.
-
bioRxiv - Cell Biology 2020Quote: ... and rabbit-anti S100B (1:500, ThermoFisher). The cells were then incubated with the secondary antibodies for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... donkey anti-rabbit Alexa488 (Thermofisher; A-21206), donkey anti-rabbit Alexa594 (Bethyl ...
-
bioRxiv - Cell Biology 2019Quote: ... rabbit anti-GFP(1:1000, Life Technologies), mouse anti-GFP (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Myeloperoxidase (MPO; Thermo Fisher Scientific), rat anti-K8 (Troma I ...
-
bioRxiv - Cell Biology 2020Quote: ... Alexa 488 rabbit (Thermo Fisher, A-11034) and Alexa 488 mouse (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 anti-rabbit (Life Technologies), and Alexa Fluor 647 anti-mouse (Life Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... goat anti-rabbit Alexa Fluor 594 (ThermoFisher), at 1:3000 dilution in blocking solution for detection.