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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... 1mL of Trizol reagent (Invitrogen) was added to each cell pellet and vortexed briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 mM NaCl (Thermo Fisher, no. AM9760G)
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bioRxiv - Synthetic Biology 2024Quote: ... Each biological triplicate RNA was run in a technical triplicate in a MicroAmp Fast Optical 96-well plate (Life Technologies). 1ng of RNA was added to Luna Universal One-Step qRT-PCR mix (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was purified from cell pellets via Trizol reagent (Invitrogen). 1mL of Trizol reagent (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... The concentration of DNA was measured on a Nanodrop spectrophotometer (Thermo Fisher Scientific), and this was used to calculate concentration of nanostructure given total DNA molecular weight.
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bioRxiv - Synthetic Biology 2024Quote: ... and enough OptiMEM (ThermoFisher/Gibco 31985062) to bring the mix volume up to 25 μL ...
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bioRxiv - Genomics 2024Quote: ... Amplifications were performed on a Veriti 96-Well thermal cycler (Applied Biosystems) using an initial denaturation at 95°C for 12 min followed by 35 cycles of denaturation at 95°C for 30 s ...
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bioRxiv - Synthetic Biology 2024Quote: ... and enough OptiMEM (ThermoFisher/Gibco 31985062) to bring the mix volume up to 25 μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... using PureLink RNA Mini Kit (Invitrogen), RNA was isolated following the protocol for ‘Purification of RNA from liquid samples’ starting from Step 2-Bind RNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then selected with 6 μg/mL blasticidin (Alfa Aesar/ThermoFisher J61883) for 7 d ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA was isolated (Dynabeads, Invitrogen, 61006) from lysed B cell cultures and RT-PCR was performed for the conversion to cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... with One Shot™ TOP10 (ThermoFisher Scientific) for allele-specific sequencing.
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bioRxiv - Neuroscience 2024Quote: ... and 1% non-essential amino acids (Thermo Fisher, 11140050). WT and pink1 KO MEF cells have been described previously [28] ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was extracted using Extracta DNA prep for PCR (Quantabio) from a sample of each clone upon passage and amplified for sequencing using Taq PCR Master Mix (ThermoFisher Scientific) at the cut site using the following primers ...
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bioRxiv - Neuroscience 2024Quote: ... 1X Non-essential Amino acid (Gibco, 11140-035), 1X Sodium pyruvate (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... no magnesium (Gibco, 14190094). After dissection of the head and all internal organs ...
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bioRxiv - Neuroscience 2024Quote: ... Stability and consistency of the gradient was monitored throughout the experimental sessions by supplementing the chemotactic solution with Alexa633 (100 nM, ThermoFisher). The dye was excited with a 633 nm diode laser (Vortran Laser Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Cell lysates (10 μg for HEK293E cells or 15 μg for MEFs) were diluted in Laemmli buffer (62.5 mM Tris, pH 6.8, 1.5% SDS, 8.33% glycerol [Fisher Scientific, BP2291] ...
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bioRxiv - Neuroscience 2024Quote: ... with CellTracker Green CMFDA and CellTracker Orange CMTMR (10 μM, Invitrogen) in a culture medium at 37° C for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... sodium pyruvate (Fisher Scientific, 11360-070), non-essential amino acids ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were digested for 15 min at 37 °C with digestion medium [0.025% Trypsin-EDTA (Gibco, 25300054); 0.125mg/mL DNase I (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... 1% PenStrep (Thermo Fisher, 15140122), and 1% non-essential amino acids (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: A total of 12 WT and pink1 KO mice (N = 3 per sex/genotype) at age of three months were anesthetized and intracardially perfused with cold PBS (Invitrogen, 14190-235). Brains were removed and immediately flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2024Quote: WT and PINK1 KO HEK293E cells were previously described [13] and were cultured in Dulbecco’s modified Eagle medium (Thermo Fisher, 11965118) supplemented with 10% fetal bovine serum (Neuromics ...
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bioRxiv - Neuroscience 2024Quote: ... 20% heat-inactivated Fetal Bovine Serum (Gibco, 10500064); 1% Penicillin-Streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... frozen brain tissues were homogenized in 5x volumes of ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors using glass Dounce tissue grinders (Fisher Scientific, K885300-0002). After 30 minutes incubation on ice ...
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bioRxiv - Neuroscience 2024Quote: ... 4°C at 20817 x g and protein concentrations were determined by BCA assay (Thermo Fisher, 23225). Cell lysates (10 μg for HEK293E cells or 15 μg for MEFs ...
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bioRxiv - Neuroscience 2024Quote: ... tissue dissociation was completed by pipette trituration and trypsin neutralised with 5 ml of culturing media [DMEM (Gibco, 11960-085); 20% heat-inactivated Fetal Bovine Serum (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... in HBSS (Gibco, 14025050)] ...
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bioRxiv - Neuroscience 2024Quote: ... B5525]) and subjected to SDS-PAGE using 8-16% Tris-glycine gels (Invitrogen, EC60485BOX). Proteins were transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... 1% L-Glutamine (Gibco, 25030024); 1X Non-essential Amino acid (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Penicillin-Streptomycin (Gibco, 15140122), 1% L-Glutamine (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 1X Sodium pyruvate (Gibco, 11360-039)] ...
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bioRxiv - Systems Biology 2024Quote: ... and SYTOXTM AAdvanced Dead Cell Stain (Invitrogen S10349), simultaneously ...
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bioRxiv - Microbiology 2024Quote: ... and 1X penicillin-streptomycin solution (Invitrogen) and maintained at 37 °C in a humidified incubator with 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal bovine serum (GIBCO) and 1X penicillin-streptomycin solution (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... we utilised 44 μL of Dynabeads™ MyOne™ Streptavidin C1 (Invitrogen) per lysate ...
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bioRxiv - Systems Biology 2024Quote: ... and random hexamers (48-190-011, Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Systems Biology 2024Quote: ... cells were stained with Annexin V conjugated with Alexa Fluro 568 (Invitrogen A13202), CellEventTM Caspase 3/7 detection reagent (Invitrogen C10423) ...
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bioRxiv - Systems Biology 2024Quote: ... or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). Plasmids were digested with restriction enzymes from New England Biolabs (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... which consisted of an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) and an Orbitrap Eclipse Tribrid MS (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... CellEventTM Caspase 3/7 detection reagent (Invitrogen C10423), and SYTOXTM AAdvanced Dead Cell Stain (Invitrogen S10349) ...
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bioRxiv - Systems Biology 2024Quote: ... The crude extracts were then centrifuged at 158 × g in a benchtop fixed-angle centrifuge (Thermo Fisher, Johannesburg, South Africa). After centrifugation ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL T4-DNA ligase buffer (Thermo Scientific) in a total reaction volume of 10 µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 27 µl of diluted 1 M Tris-HCl (pH 8.0) (Invitrogen #15568-025) were simultaneously added to each well ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL of T4-DNA Ligase (5 Weiss U/µL, Thermo Scientific) and BsaI (10 U/µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... 15% ES cell qualified FBS (Gibco), 1x MEM non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 mM sodium pyruvate (Thermo Fisher Scientific), 100 µM B-mercaptoethanol (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... coated with Geltrex (Life Technologies Corporation; Cat. No. A1413301). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL T4-DNA ligase (5 Weiss U/µL, Thermo Scientific), 1 µL T4-DNA ligase buffer (Thermo Scientific ...