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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... A subconfluent 6-well dish of POE SH-SY5Ys were transfected with 1ug of each PINK1_Seq1 and PINK1_Seq2 sgRNA encoding pSpCas9(BB)-2A-GFP plasmid using lipofectamine 3000 (Invitrogen). 24hr post-transfection cells were trypsinised and subjected to fluorescence-activated cell sorting and clonal selection of GFP-positive cells.
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bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 568 goat anti mouse (Invitrogen, A11004, 7 RRID:AB_2534072, 1:2000), AlexaFluor 568 anti rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... pCMVR8.74 and pLVX-EF1α-mitoSRAI-IRES-Puro at a 1:1:2 molar mass ratio using Lipofectamine 3000 (Invitrogen). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody staining was performed at 37°C overnight with the following antibodies: Anti-GFP (Life Technologies A10262), Anti-NMDAR1 (SYSY 114011) ...
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bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 568 anti rabbit (Invitrogen, A11011, RRID:AB_143157, 1:2000), Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in 1:5000 DAPI (Life Technologies) in PBS for 3 min ...
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bioRxiv - Neuroscience 2024Quote: ... consisting of 1:500 donkey anti-mouse Alexa Fluor 594 (Invitrogen), 1% NDS ...
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bioRxiv - Neuroscience 2024Quote: ... Hoechst 33342 (Thermo Scientific, 62249, 1:10000)) ...
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bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 488 goat anti rabbit (Invitrogen, A11008 ...
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bioRxiv - Neuroscience 2024Quote: ... Hoechst 33342 (Thermo Scientific), and DRAQ5 were all at a 1:2000 dilution in 10% FBS/PBS ...
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bioRxiv - Neuroscience 2024Quote: ... in Dulbecco’s modified Eagle medium (DMEM, Gibco, 11995-065) with 4.5 g/L D-glucose and L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... GFP: anti-Chicken-488 (Invitrogen A11039); NMDAR1 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500 rabbit anti-GFAP (Invitrogen), 1% NDS ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500 donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific), 1% NDS ...
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bioRxiv - Neuroscience 2024Quote: ... then transferred to 30% sucrose (Fisher Scientific) in solution for cryoprotection and remained in the solution until sectioning ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in a secondary antibody solution containing 1:200 donkey anti-mouse Alexa Fluor 488 (Invitrogen, Burlington, ON, Canada) or 1:200 donkey anti-goat Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation in 2N HCl (Fisher Scientific) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Before imaging, cell media was replaced with 10% FBS (Gilbco, 10500-064) phenol-free DMEM (Gibco, 31053-028) containing the previously determined concentrations of treatments ...
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bioRxiv - Neuroscience 2024Quote: ... both compounds were diluted in DMEM (Gibco, 11995-065) so that 25uL of each diluted compound was added per well of a 96-well plate (PhenoPlate 96-well microplate ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-HUC/D (pan-neuronal marker) (1:30) ( Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... The cell-permeant Mitotracker (M22426, ThermoFisher) was used at a concentration of 100 nM ...
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bioRxiv - Neuroscience 2024Quote: ... and AMPAR1 and AMPAR2: anti-Rabbit-AF+647 (Invitrogen A32733). Final expansion was performed just before imaging by putting the trimmed slices in 0.1 mM tris in distilled water ...
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bioRxiv - Neuroscience 2024Quote: ... cells were loaded with 1 μm Fura-2AM supplemented with 0.01% Pluronic F-127 (w/v, Life Technologies) in a physiological Ringer’s solution containing the following (in mm) ...
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bioRxiv - Neuroscience 2024Quote: Samples were analyzed on a Waters nanoACQUITY UPLC coupled to a QExactive mass spectrometer (Thermo Scientific, Bremen, Germany) equipped with a nano-electrospray ion source ...
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bioRxiv - Neuroscience 2024Quote: ... The column (µPAC™ trapping column, Thermo Scientific) was loaded with 5 µl of sample and set to a flow rate of 750 nl per minute ...
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bioRxiv - Neuroscience 2024Quote: ... or donkey anti-rabbit Alexa Fluor 488 (1:500, Invitrogen). Washed sections were then counterstained with Hoechst 33342 for 10 minutes (1:5,000 in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Washed sections were then counterstained with Hoechst 33342 for 10 minutes (1:5,000 in PBS, Molecular Probes), mounted onto slides ...
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bioRxiv - Plant Biology 2024Quote: ... measured RNA concentration using a Qubit 1.0 instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the RNA HS assay ...
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bioRxiv - Neuroscience 2024Quote: ... the Click-iTTM EdU Cell Proliferation Kit (Thermo Fisher Scientific, catalog # C10338) was used according to manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2024Quote: ... and dry pellet was analyzed by a Nicolet IS10 spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2024Quote: ... and GDSL-TYPE ESTERASE were PCR amplified with PfuUltraII polymerase and cloned into pENTR (ThermoFisher), then recombined into pMDC11165 to create promoter::GFP fusions (primers are listed in Supplemental Table 13) ...
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bioRxiv - Plant Biology 2024Quote: ... constructs were integrated into destination vectors pGTQL1221YC or pGTQL1211YN by LR recombination using Gateway LR clonase II (Invitrogen). The constructs were transformed into A ...
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bioRxiv - Plant Biology 2024Quote: ... Coding regions of IPI-O1 and IPI-O1nlswithout a signal peptide and StCWC15a/b were amplified with primers containing attB tails and BP-cloned into pDONR207 (Invitrogen). To generate BiFC constructs ...
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bioRxiv - Plant Biology 2024Quote: ... using chitosan oligosaccharides conjugated with AlexaFluor 488 hydroxylamine (ThermoFisher, Waltham, Massachusetts, USA). In brief ...
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bioRxiv - Physiology 2024Quote: ... were fixed in Fixx solution (Thermo Scientific, 9990244) for 15 min and permeabilized in 0.1% Triton X-100 (Amresco ...
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bioRxiv - Physiology 2024Quote: ... 20 % Fetal Bovine Serum (Gibco, 10270-106) and 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA samples quantity was assessed using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Invitrogen™) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... and 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco, 15140-122). To induce differentiation ...
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bioRxiv - Physiology 2024Quote: ... CD45 (1:200, Life Technologies), coupled with Alexa-488 or Alexa-568 fluorochromes (Life Technology ...
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bioRxiv - Physiology 2024Quote: ... 20 % Fetal Bovine Serum (Gibco, 10270-106) and 100 U/mL penicillin and 100 mg/mL streptomycin (Gibco ...
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bioRxiv - Physiology 2024Quote: ... Trypsin-EDTA 0.05% (GIBCO, 25300-062) was used to detach cells ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed with the High-Capacity RNA-to-cDNA Kit (4387406 Thermofisher scientific) as shown previously44 ...
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bioRxiv - Physiology 2024Quote: ... Protein synthesis was measured according to the instructions of the Click-iT HPG kit (C10428, Thermofisher Scientific). All cell lines were regularly tested for mycoplasma and inspected for fungi contamination.
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bioRxiv - Plant Biology 2024Quote: Liquid chromatography was performed on a Vanquish Horizon UHPLC System by Thermo Fisher (Waltham, MA, USA) build from a Vanquish binary pump H ...
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bioRxiv - Neuroscience 2024Quote: ... Twenty grams of each sample were loaded onto a gel (NuPAGE 4 to 12%, Bis-Tris) (Invitrogen, Waltham, MA, USA). NuPAGE LDS sample buffer (1:4 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA were extracted from shoot or root tissues using TRIZOL (Invitrogen, USA), according to the manufacturer recommendations ...
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bioRxiv - Plant Biology 2024Quote: Instrument control was done using Hystar (Bruker, version 6.0) containing a Chromeleon Plug-In (Thermo Fisher, plugin version 1.3.8 ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was treated with Protease QS (Thermo Fisher, QVP0011) diluted 1:2000 in PBS for 10 minutes at room temperature while shaking ...
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bioRxiv - Cell Biology 2024Quote: LC-MS/MS analysis was performed on an Q Exactive mass spectrometer (Thermo Scientific) equipped with a Digital PicoView source (New Objective ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred onto nitrocellulose membranes using an iBlot2 gel transfer device (Thermo Fisher Scientific, Waltham, MA, USA). The membranes were then blocked with 5% nonfat milk in PBST at room temperature for 30 min ...