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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... was coupled to a Q Exactive Orbitrap mass spectrometer through a heated electrospray ionization (HESI II) source (Thermo Scientific). Source and capillary temperatures were 300°C ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Cells were transfected with these vectors following the Lipofectamine 2000 (ThermoFisher Scientific) and selected with puromycin (250 ng/mL ...
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bioRxiv - Systems Biology 2024Quote: ... was coupled to a Q Exactive HF Orbitrap mass spectrometer through a heated electrospray ionization (HESI II) source (Thermo Scientific). Source and capillary temperatures were 350°C ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... that were equilibrated 5x with H2O and the eluted volume was transformed into 20 µL of MegaX DH10β cells (Thermo Fisher) by electroporation ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Oligo pools were PCR-amplified using Phusion Flash High-Fidelity PCR Master Mix (ThermoFisher Scientific) according to the manufacturers protocol with 1 ng/μL sgRNA template DNA ...
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bioRxiv - Systems Biology 2024Quote: The LC system (Vanquish Binary Pump, Thermo Scientific) was coupled to a Q Exactive HF Orbitrap mass spectrometer through a heated electrospray ionization (HESI II ...
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bioRxiv - Systems Biology 2024Quote: ... We stripped the membranes using the Restore™ western blot stripping buffer (Thermo Fisher Scientific™, Waltham, USA) before being re-examined ...
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bioRxiv - Systems Biology 2024Quote: ... 20 µl of all fractions were taken for Western analysis while the rest were mixed with Trizol LS (10296028, Thermo Fisher Scientific) for subsequent RNA isolation.
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bioRxiv - Systems Biology 2024Quote: ... 100 µM β-mercaptoethanol (21985023, Thermo Fisher Scientific), 3 μM CHIR99021 (SML1046-5MG ...
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bioRxiv - Systems Biology 2024Quote: ... connected to an EASY-nLC 1200 system (Thermo Scientific). This LC system was configured in a trapping setup ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting peptide concentration was determined by BCA assay (Pierce, Thermo Scientific).
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bioRxiv - Systems Biology 2024Quote: ... The resulting peptide concentration was determined in a colorimetric assay using bicichoninic acid (BCA) (Pierce, Thermo Scientific).
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bioRxiv - Systems Biology 2024Quote: Data-dependent label-free analysis of soluble protein fractions was performed using an Ultimate 3000 RSLC nanosystem (Thermo Scientific, Waltham, MA) coupled to a Fusion mass spectrometer (Thermo Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... The quantity and integrity of dsRNA were measured by Nanodrop spectrophotometer (Thermo Scientific, Waltham, MA) and agarose gel electrophoresis.
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bioRxiv - Systems Biology 2024Quote: ... Each feeding involved the administration of a pellet composed of calf liver puree and low melting agarose (Thermo Fisher Scientific) at a ratio of 1:1 ...
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bioRxiv - Systems Biology 2024Quote: ... and DAPI (ThermoFisher, 1:100) in PBST for 2h at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... A volume of 10 µl of the Alamar blue (Thermofisher, USA) reagent was added to each well following the 24 hours treatment and the plate was incubated for 2 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were treated with TrypLE express (Gibco #12605010) at 37 C for 5 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... DyLight™ 650) and DAPI (ThermoFisher, #D1306, 300 nM) in PBS-T + 0.1% BSA for 1 hour at RT ...
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bioRxiv - Systems Biology 2024Quote: Expi293F cell (Gibco) lysate was submitted to in-solution LysC/trypsin digestion as previously described15 ...
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bioRxiv - Systems Biology 2024Quote: ... hCoV-19/USA-WA1/2020) at an MOI of 5 (titered in VeroE6 cells) in phosphate-buffered saline containing calcium and magnesium (PBS++; Gibco, 14040-133) and incubated at 37°C for 1.5 hours ...
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bioRxiv - Systems Biology 2024Quote: ... cells were resuspended in 400-500 μL of buffer and underwent labelling with 1μL/mL of a 1mg/mL stock solution of Alexa Fluor 488-Conjugated Concanavalin A (Invitrogen) for cytoplasm staining ...
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bioRxiv - Systems Biology 2024Quote: Data acquisition of surface protein markers was performed on the Attune NxT Flow Cytometer (ThermoFisher Scientific). PD-GSCs were dissociated from their respective substrate using Accutase and washed twice with PBS + FBS serum (10%) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL T4-DNA ligase buffer (Thermo Scientific) and approximately 25 fmol of each precursor plasmid ...
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bioRxiv - Systems Biology 2024Quote: ... Stem cells were transduced with lentivirus packaged with CROPseq-Guide-Puro vectors and selected with Puromycin (Gibco; Cat. No. A11138-03) until confluent ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plates were incubated at room temperature for 45 minutes then absorbances at 405nm and a 600nm wavelength control were measured using a microplate spectrophotometer (Multiskan GO microplate reader, Thermo Fisher).
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected using Lipofectamine 3000 (L3000001, Thermo Fisher Scientific) at the manufacturer recommended high concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... and BsaI (10 U/µL, Thermo Scientific) and 1 µL T4-DNA ligase buffer (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Eluting peptides were electro sprayed into an Orbitrap Ascend Tribrid mass spectrometer (Thermo Fisher Scientific), using Data Dependent (DDA) ...
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bioRxiv - Systems Biology 2024Quote: NanoLC connected to an Orbitrap Exploris mass spectrometer (Thermo Fisher Scientific) was used for the analysis of all samples ...
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bioRxiv - Synthetic Biology 2024Quote: ... the gels were either stained with 0.5 µg/mL of ethidium bromide solution or 1X SYBR safe (Thermo Fisher Scientific) and followed by de-staining in Milli-Q water for 15 minutes ...
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bioRxiv - Systems Biology 2024Quote: Non-transformed human mammary epithelial cells (MCF-10A, ATCC) were maintained in growth medium consisting of DME medium/Ham’s F-12 (ThermoFisher) containing HEPES and L-glutamine supplemented with 5% (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 µg RNA starting material was reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Germany). Gene expression was analyzed with predesigned QuantiTect primer assays (Qiagen ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 cells were obtained from Thermo Scientific. HEK293T and MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Systems Biology 2024Quote: ... The peptide separation was carried out using a Proxeon EASY nLC 1200 System (Thermo Fisher Scientific) fitted with a custom-made C18 column (15 cm x 150 μm ID ...
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bioRxiv - Systems Biology 2024Quote: ... and 1% penicillin-streptomycin (ThermoFisher), as described previously49 ...
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bioRxiv - Systems Biology 2024Quote: ... and Tandem Mass Tag (TMT) isobaric label reagent (cat#: 90066 for TMTsixplex and cat#90406 for TMT10plex) were purchased from Thermo Scientific. FuGene 6 (cat# ...
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bioRxiv - Systems Biology 2024Quote: Cells were lysed utilizing RIPA lysis buffer (Thermo Scientific) comprising 25 mM Tris-HCl ...
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bioRxiv - Systems Biology 2024Quote: ... implemented in Proteome Discoverer 3.0 software (Thermo Fisher Scientific). All experiments used standard parameters and filtered to 1% FDR protein and peptide level ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were analyzed using 4-20% Novex Tris-Glycine mini gels (Invitrogen), followed by transfer onto a 0.2 µM polyvinylidene fluoride (PVDF ...
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bioRxiv - Systems Biology 2024Quote: ... and L-glutamine (2mM, Gibco). All the cell lines were tested negative for mycoplasma contamination.
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bioRxiv - Systems Biology 2024Quote: ... containing HEPES and L-glutamine supplemented with 5% (v/v) horse serum (ThermoFisher), 20 ng/ml EGF (Peprotech) ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were then dried down in a SpeedVac vacuum evaporator and resuspended in 1.07µl of 0.1% Formic Acid (buffer A) and tightly sealed using an aluminium foil cover (Thermo Fisher AB0626).
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bioRxiv - Systems Biology 2024Quote: Peptides were separated on Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) using an in-house packed 50μm ID x 50cm L Reprosil-Gold C-18 (Dr ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids with pcDNA-based backbones (including transcription unit positioning vectors, TUPVs) were transformed into chemically competent TOP10 Escherichia coli (Thermo Fisher #C404010), and cells were grown at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... HPLC data were processed using the ChromeleonTM chromatography data system software version 7.1.3 (Thermo Fisher Scientific Co.). The acetone concentration was monitored by refractive index detection ...
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bioRxiv - Synthetic Biology 2024Quote: ... The DNA sequence between the XbaI (Thermo Scientific, FD0684) and XhoI (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The protein concentration was measured using NanoDrop (Thermo Fisher Scientific) and the protein was aliquoted and stored at -80 °C until use.
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bioRxiv - Synthetic Biology 2024Quote: ... The OD600 values in shaken-flask cultures were periodically recorded in a GENESYSTM 20 spectrophotometer (Thermo Fisher Scientific Co.) to estimate bacterial growth ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel was stained using SimplyBlue stain (Invitrogen) and imaged by a Sapphire Biomolecular Imager (Azure biosystems ...