Labshake search
Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... All samples were then cleaned with SequalPrep Normalization plate kit 96-well (Invitrogen, Carlsbad, CA, USA), pooled and sequenced on MiSeq (Illumina ...
-
bioRxiv - Immunology 2024Quote: ... The following day PlatE cells were transfected with pBMN-plasmids using a Lipofectamin 3000 kit (Invitrogen) according to the manufactures protocol ...
-
bioRxiv - Systems Biology 2023Quote: Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products were purified and normalized using the SequalPrep normalization plate kit (Thermo Scientific, MA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: GST or GST-PHF1-tudor proteins (10 μg each) were incubated with 4 μl (25% slurry) of Glutathione Magnetic Beads (88821; Thermo Scientific) in 250 μl of binding buffer (125 mM Tris-HCl pH8.0 ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 mg clarified mitochondrial lysates prepared in lysis buffer was incubated overnight with 50 µl glutathione-conjugated magnetic agarose beads (Thermo Scientific) at 4°C on a spinning rotor ...
-
bioRxiv - Biophysics 2020Quote: ... and unprocessed H57-scFV (still containing a 12x histidine tag) were removed by a batch purification with Ni-NTA agarose (ThermoFischer Scientific) and glutathione agarose (ThermoFisher Scientific). The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL ...
-
bioRxiv - Biophysics 2020Quote: ... Eluted protein fractions were site-specifically biotinylated using the BirA (Acro Biosystems Inc.) and subjected to a batch purification step with glutathione agarose (ThermoFisher Scientific) to remove the BirA ligase and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
-
bioRxiv - Microbiology 2020Quote: ... Purified proteins were dialyzed in equilibration buffer (50 mM Tris, 150 mM NaCl, pH 8.0) and incubated with Pierce® Glutathione Spin Columns (Thermo Scientific) at RT for 1 hour with gentle rotation according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... were amplified via the polymerase chain reaction (PCR) using primers to facilitate subsequent ligation of the PCR products into the glutathione-S-transferase encoding vectors (Life Technologies). The M.MspI PCR product was initially inserted into the BamHI and EcoRI sites in pGEX 2T ...
-
bioRxiv - Immunology 2020Quote: ... Bacterial lysate was clarified by centrifugation at 40,000 xg for 45 min at 4°C and the clarified protein bound to glutathione agarose beads (Thermo Fisher Scientific) at 4 °C for 4 hours with rotation ...
-
bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: BJ human skin fibroblast cells were grown in a humidified 37°C incubator containing 5% CO2 in MEM medium containing glutathione and high concentrations of vitamins (catalog no. 11875093, Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
-
Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...
-
bioRxiv - Cell Biology 2024Quote: The binding affinity of Halo-CCSer2650–850 for Ndel1 was determined by coupling GST-Ndel1 to 30 μL of Glutathione Beads (Thermo Scientific) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein mixture was left on ice for 15 min then incubated with binding buffer-equilibrated glutathione agarose beads (Invitrogen, G2879) on a rotator for 1h at 4 ℃ ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were sorted in 96-well plates (Piko PCR Plates 24-well, Thermo Scientific, SPL0240 and Plate Frame for 24-well PikoPCR Plates, Thermo Scientific, SFR0241). Each well contained Triton-X100 (0.2% ...
-
bioRxiv - Cancer Biology 2019Quote: ... cell apoptosis were measured by double-staining with Annexin V-fluorescein isothiocyanate (Annexin V-FITC) and Propidium Iodide (PI) by Apoptosis Detection Kit (Invitrogen, Carlsbad, California, USA) and analyzed by flow cytometry (Beckman Cytoflex ...
-
bioRxiv - Genomics 2019Quote: ... Genomic DNA concentration was measured using the Qubit fluorimetry system with the High Sensitivity kit for detection of double-stranded DNA (Thermo Fisher, Part #Q32854), and fragment size distribution was assessed using the 2100 Bioanalyzer with the 12000 DNA kit (Agilent ...
-
bioRxiv - Microbiology 2019Quote: ... click chemistry and fluorescence detection were based on recommendations from Click-iT® HPG Alexa Fluor® Protein Synthesis Assay Kits (Molecular probe by Life Technologies). HUVECs were grown on chambered coverslip slides (Ibidi ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membranes were probed with species-specific donkey anti-rabbit or goat anti-mouse HRP-conjugated secondary antibody and detected with enhanced chemiluminescence (ECL) detection kit (Thermo Fisher, Carlsbad, CA).
-
bioRxiv - Bioengineering 2019Quote: ... Aliquots of the solution were then sampled for glucose detection using the Amplex™ Red glucose/glucose oxidase assay kit (ThermoFisher cat.no. A22189) according to manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
-
bioRxiv - Neuroscience 2020Quote: The EV-mediated activation of apoptosis was also assessed using an in-vivo fluorescence microscopy assay designed to detect the abundance of active caspases in live cells (Image-iT™ Live Green Poly Caspases Detection Kit, cat. no. I35104, Thermo Fisher Scientific). This assay is based on the cell permeant and noncytotoxic FLICA™ fluorescent inhibitor of caspases that binds covalently to the enzymatic reactive center of activated caspases ...
-
bioRxiv - Cell Biology 2021Quote: ... Membranes were then cross-linked at 120J/m2 and then labelled RNA was detected using the Chemiluminescent Nucleic Acid Detection module kit (Thermofisher, cat n°: 89880) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Twenty-four surviving single cell clones for each crRNA were tested for indels using the GeneArt™ Genomic Cleavage Detection Kit (ThermoFisher, Cat# A24372). Positive clones were Sanger sequenced ...
-
bioRxiv - Microbiology 2023Quote: ... and the viral genomic copies were quantified using a real-time reverse transcription PCR (RT-PCR) (VetMax-Gold SIV Detection Kit, Life Technologies, Austin, TX). A standard curve was established using a chemically synthesized RNA fragment with known copy numbers ...
-
bioRxiv - Molecular Biology 2024Quote: ... using Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection with Alexa Fluor™ 594 dye (Invitrogen, Catalog number: C10618), as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Add 1.5 μM FlAsH-EDT2 labeling reagent of TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit (Thermo Fisher Scientific, MA, USA), wash twice with 250 μM BAL buffer ...
-
bioRxiv - Biophysics 2021Quote: ... we manually aligned the two IR pulsed laser beams with the 635 nm beam by inspecting the images of 1 μm multicolor fluorescent beads (T7282, TetraSpeck™ Microspheres, Thermo Fisher Scientific) diluted 1:10 in distilled water ...
-
bioRxiv - Cancer Biology 2021Quote: ... Slides were washed three times in PBS containing 0.05% Tween-20 and incubated with a secondary fluorescent antibody (all Invitrogen, concentration 1 μg/mL) for 1 hour in the dark at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed in PBS and reacted with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, 1:500, Invitrogen, catalog # A-11034) in 1% horse serum ...
-
bioRxiv - Developmental Biology 2019Quote: Samples were washed and incubated in the dark with fluorescent secondary antibody (preadsorbed goat anti-rabbit Alexa Fluor555, or goat anti-mouse Alexa Fluor488, Molecular Probes, 1:500) in PBSTA for 90 min at RT ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were then incubated for 1h with secondary antibodies conjugated with Alexa Fluor 594 fluorescent dyes (Molecular Probes, 1:400 dilution in PBS+). Then ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Primary antibodies were incubated in 1% normal goat serum at 4ºC overnight and detected with fluorescent secondary antibodies (A-11032, A-11037, A-11029, ThermoFisher Scientific, 1:500 dilution) through a 1-hour incubation at 37ºC ...
-
CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... samples were incubated with infrared beads (FluoSphere infrared fluorescent Carboxylate-Modified Microspheres, ThermoFisher, USA; ex/em 715/755□nm; 1:2,000 in PBS) for 10 min and then PBS was exchanged with dSTORM buffer containing 100 mM mercaptoethylamine (MEA ...
-
bioRxiv - Neuroscience 2019Quote: ... Spinal cord free-floating sections were stained with 1:300 dilution of NeuroTrace 500/525 green fluorescent Nissl stain (Molecular Probes, Eugene, OR) for 1 h ...
-
bioRxiv - Immunology 2021Quote: ... biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
-
bioRxiv - Physiology 2022Quote: ... rinsed 3x for 5min in 0.1M PBS and incubated in fluorescent secondary antibodies (Alexa Fluor® 488; goat anti-mouse; 1:750 dilution; Thermo Fisher A-11034) for 2hr at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... then washed in 1x PBS and incubated for 20 min at RT with fluorescent Nissl Stain (NeuroTrace 435/455, Thermo Fisher, 1:150). Afterwards ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were either Nissl-stained with cresyl violet or stained with the fluorescent Nissl reagent NeuroTrace Blue (1:200) (Life Technologies, N-21479). In four subjects ...
-
bioRxiv - Neuroscience 2021Quote: ... The sections were briefly washed in PBS and stained with Neurotrace blue fluorescent Nissl stain (1:500, Thermo Fisher Scientific, Waltham, MA, USA) in 0.3% Triton-X100/PBS for overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
-
bioRxiv - Microbiology 2019Quote: ... the planktonic cells were aspirated and biofilms were gently washed twice with PBS and stained with fluorescent dye FUN-1 (Invitrogen, Thermo Fisher Scientific, ZA) and concanavalin A (ConA)-Alexa Fluor 488 conjugate (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... sequential 200 μm-apart spinal cord sections were fluorescently labeled with rabbit anti-Iba1 (#019-19741, Wako; 1:200) and FluoroMyelin™ Green Fluorescent Myelin Stain (#F34651, Thermo Fisher Scientific). For automated imaging ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated with the following fluorescent secondary antibodies at RT for 2h: Goat anti-mouse Alexa Fluor 488 (1:200, Thermo Fisher A-11029), Streptavidin Alexa Fluor 647 (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated with the following fluorescent secondary antibodies at RT for 4h: Goat anti-mouse Alexa Fluor 488 (1:200, Thermo Fisher A-11029), and counterstained with a nuclear stain ...