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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The following siRNA target sequences were used: GTPBP5 siRNA: 5’-CGGUGGACACGUCAUUCUGTT-3’ (134621, Ambion); GTPBP7 siRNA ...
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bioRxiv - Neuroscience 2024Quote: ... larvae at 5 dpf were incubated in 3 µM FM 1-43 (Thermofisher, T3163) in E3 media for 35 s ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... were cultured for up to 7 days at 37°C in 5% CO2 in RPMI 1640 media (Gibco) supplemented with 10% Heat Inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Systems Biology 2022Quote: ... 4 × 108 cells per biological replicate were used after 7 days of blasticidin selection (10 mg/mL, Gibco), which was 9 days post-infection ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells previously labeled with CMAC cell tracer (7-amino-4-chloromethylcoumarin, CellTracker Blue CMAC Dye, Invitrogen) were added to the microscope (Nikon ECLIPSE Ti-TimeLapse microscopy) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... The lumen of the vacuole was stained by adding 20 µM 7-amino-4-chloromethylcoumarin (CMAC) dye (Invitrogen) to 0.5 OD Units and incubating them for 15 min at 30°C with shaking ...
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bioRxiv - Cell Biology 2024Quote: ... 7 µg of the samples were loaded onto Bolt™ 4-12% Bis-Tris Protein Gels (Invitrogen, NW04120BOX) and transferred to a PVDF membrane using the iBlot™ 2 Transfer Stacks and iBlot2 system (Method ...
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bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Developmental Biology 2021Quote: Preparation of lipid/cholesterol depleted fetal bovine serum (FBS): 500mL FBS were stirred overnight at 4°C with 10g Cab-osil M-5 (ACROS Organics 7631-86-9). The mix was then centrifuged for 10min at 3000rpm and the supernatant filtrated under sterile conditions ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated on ice for 5 min before cellular debris was pelleted by centrifugation at 10,000 rpm for 5 min at 4°C and supernatant was removed (Thermo Scientific, Heraeus Pico 21 centrifuge). Centrifugation was repeated twice more to remove fat from the samples ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... the cells were washed in ice-cold sterile PBS (650g, 5 min, 4°C) and stained for 30 min with fixable viability dye eFluor 780 (Thermo Fisher Scientific, 1:1000) using heat-killed (1 min ...
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bioRxiv - Genomics 2022Quote: ... bone marrow was extracted and rinsed in a small glass Petri dish with 5 ml chilled Roswell Park Memorial Institute (RMPI) 1640 (Gibco, chilled at 4°C) culture medium and then transferred to a centrifuge tube containing 10 ml RPMI ...
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bioRxiv - Neuroscience 2023Quote: ... or Tris-Glycine-SDS sample buffer (Novex) at 95°C for 5 min and separated using 4–20% Tris-glycine (ThermoFisher, #EC6025 or Invitrogen # XP04200). Proteins were transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were first boiled with 6x Laemmli SDS buffer at 95°C for 5 minutes and then proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX), transferred to Nitrocellulose membranes using the Mini Trans-Blot Cell (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... samples were first boiled with 6x Laemmli SDS buffer ar 95°C for 5 minutes and proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX). Gels were washed in water ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured at 100 °C for 5 minutes and briefly centrifuged before loading onto a 4-12% Tris-Glycine Novex Wedgewell gel (Fisher Scientific, Cat no. XP04125BOX). SDS-PAGE was performed using standard running buffer in Xcell Surelock Electrophoresis Cell (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was collected by centrifugation at 300x g at 4°C and incubated in 5 mL of 1x RBC lysis buffer (Thermo Fisher Scientific, Massachusetts, USA) per spleen for 5 min at room temperature with occasional shaking to remove blood cells ...
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bioRxiv - Microbiology 2022Quote: ... The F gene was PCR amplified using primers RSV F 5’ (5’GCAAGGATTCCTTCGTGAC3’) and RSV F 3’ (5’CACACCACGCCAGTAG3’) and Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). Sanger sequencing was performed by Elim Biosciences.
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bioRxiv - Physiology 2024Quote: ... and stored at 4 °C overnight prior to storage at −80 °C following RNAlaterTM best practices (Life Technologies, 2011). Following this ...
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bioRxiv - Neuroscience 2021Quote: ... The CVs were stored overnight at 4°C in RNAlater (ThermoFisher Scientific, Waltham, MA), then transferred in an empty tube and stored at −80°C.
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bioRxiv - Genomics 2020Quote: ... at 4°C and in secondary antibody plus DAPI (1:1000, Invitrogen, ThermoFisher Scientific) was 3h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... This mixture was incubated overnight at 4°C with beads (Protein A Dynabeads, Invitrogen, for CTCF ChIP ...
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bioRxiv - Genomics 2020Quote: ... at 4°C and in secondary antibody plus DAPI (1:1000, Invitrogen, ThermoFisher Scientific) was 3h at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... and 4 °C cooling hold step) in an ABI StepOnePlus Detection System (Applied Biosystems). ΔΔCt values for genes were examined using Ct values generated by StepOnePlus software (Applied Biosystems).
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in 4 °C 1x phosphate-buffered saline (PBS, Thermo Fisher Scientific 10010023) to generate cell suspensions used for scWB and scIEF.
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C followed by washes with 1xDPBS (Gibco™, Thermo Fisher Scientific). Cells were activated with LPS (1 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C followed by washes with 1xDPBS (Gibco™, Thermo Fisher Scientific). Cells were activated with LPS (1 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight at 4 °C and with secondary antibodies and Alexa Fluor 633 Phalloidin (Invitrogen) for 1 h at room temperature on a shaker.
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bioRxiv - Cancer Biology 2021Quote: ... overnight at 4°C and secondary antibody 1/200 anti-IgG-Alexa568 (A11036, Thermofisher) during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... at 4°C and 100% humidity in a Vitrobot Mk IV (Thermo Fisher Scientific). Sample excess were removed by blotting for 3 s using a blot force of 0 ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated at 4 □C for 15min with Aqua viability dye (Life Technologies) and then subsequently incubated at 4°C for 30 min with either control isotype Ab or appropriate surface marker (MerTK ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C and supernatant was quantified with enhanced BCA protocol (Thermo Fisher Scientific, Pierce). Equivalent amounts of proteins were separated on an SDS-page (in general 20 μg increased to 50 μg for pSTAT1 Y701 evaluation after IR treatments ...
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Low-cost drug discovery with engineered E. coli reveals an anti-mycobacterial activity of benazeprilbioRxiv - Synthetic Biology 2021Quote: ... cooled to 4 °C and lysed with 10 mL B-PER reagent (ThermoFisher 78248) and a standard EDTA-free protease inhibitor cocktail (Merck 11873580001).
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4 °C and detected with Alexa-488-labeled secondary antibody (Life Technologies). Images were taken using a Zeiss inverted fluorescent microscope ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4°C and with secondary antibodies Alexa488 (1:500, Invitrogen Molecular Probes), Alexa568 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... overnight at 4°C and with secondary antibodies Alexa488 (1:500, Invitrogen Molecular Probes), Alexa568 (1:500 ...
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bioRxiv - Genomics 2020Quote: ... at 4°C and washed twice with ice cold HBSS (ThermoFisher, Cat. N: 14025092). After the final wash cells were resuspended in PBS with 1% BSA (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C and then transformed into TOP10 competent cells (Thermo Fisher Scientific). Positive colonies that were confirmed by colony PCR were grown in LB broth overnight with ampicillin ...
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bioRxiv - Immunology 2020Quote: ... Bead-conjugation was carried out at 4°C in Phospho-buffered saline (PBS, Gibco), for 1 hour with regular agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... an overnight incubation was performed at 4°C with Phalloidin-Rhodamin (1/200, ThermoFisher) or Phalloidin-Alexa488 (1/200 ...