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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... or CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific) to label apoptotic cells (Caspase-3/7 activity-positive and SytoxAADvanced-negative ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptotic cells were detected with CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500) ...
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bioRxiv - Cell Biology 2022Quote: ... in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio, 1:100 N2 Supplement [Gibco], 1:50 B27 Supplement [Gibco], 2 mM Glutamax [Gibco] ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transfer to a new tube ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibody solution for 1 hour (Goat anti-Mouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034 ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5X Maxima H Minus RT Buffer (Thermo Fisher), 1 μl RNaseOUT (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS and further incubated in the dark for 2 h with Alexa Fluor® 488-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Scientific; 1:500). Then ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse and/or goat anti-rabbit AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500) secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with 1:2500 Alexa Fluor ® 568 F(ab’)2 fragment of goat anti-Rabbit IgG (H+L) (Thermo Scientific # A-21069) cells were stained with DAPI and mounted in VECTASHIELD (VectorLaboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... a first set of sections of each type of immunostaining was processed for immunofluorescence being incubated for 2 h at room temperature in Alexa 488-coupled Streptavidin (1:200; Molecular Probes, Eugene, OR, USA). Sections were rinsed and stained with DAPI to reveal borders between layers and cytoarchitectonic areas ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated with secondary antibodies for 2 h (anti-rabbit in goat Alexa-Fluor 488 nm, 1:500, Invitrogen Thermo Fisher Scientific, USA). For the second set of slices ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were stained for 3 min with Hoechst (Invitrogen, Cat #H-3570). Cells were then analyzed by confocal microscopy on a Zeiss LSM700 inverted microscope.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were further incubated for 1 h with secondary anti-rabbit-Alexa Fluor 594 antibody (1:2000; A11072, Invitrogen) or anti-mouse-Alexa Fluor 594(1:2000 ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated for 1 h at room temperature with 1:500 donkey anti-mouse 647 secondary antibody (Life Technologies). After washing in PBST and PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were incubated for 1 h with Alexa Fluor® 488 Goat Anti-Rabbit IgG (Invitrogen A11008: 1:500) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... followed by secondary antibody incubation (1 h at room temperature) with fluorescein-labeled anti-mouse IgG (1:200, Invitrogen) in 3% normal goat serum in PBS ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with the secondary antibody 1:5000 for 1 h (HRP-coupled anti-rabbit IgG, Invitrogen). Protein detection was performed with the SuperSignal West Pico Plus kit according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated for 1 h with a secondary antibody diluted 1:500 (Alexa Fluor 488-conjugated; ThermoFisher) in 1% FBS/PBS solution ...
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bioRxiv - Microbiology 2022Quote: ... followed by a 1 h incubation at room temperature with a 1:5,000 dilution of secondary anti-rabbit-HRP antibody (Invitrogen) in TBST ...
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bioRxiv - Biophysics 2020Quote: ... the double-cysteine mutant of ClpB (or ΔNClpB) was incubated for 1 h with 1:1.2 molar ratio of Alexa Fluor (AF) 594 (C5 maleimide, Invitrogen) under native conditions (25 mM HEPES ...
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bioRxiv - Bioengineering 2020Quote: ... Actin filaments were fluorescently labeled with AlexaFluor-488-conjugated phalloidin (Life Technologies; 1: 200 dilution, 1 h at RT) for visualization ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Electroporated slices were placed for 1 h in MEM medium (10% FBS, 0.5% glucose, 1% Pen/Strep, in MEM with glutamine, all Gibco) in a 5% CO2 humidified incubator at 37 °C and then incubated in Neurobasal/B27 medium for 18-24 h followed by the time lapse imaging (Denaxa et al. ...
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bioRxiv - Genetics 2022Quote: ... Sheep IgG (H+L) Highly Cross-Adsorbed Donkey anti-Ovine HRP (1:2000-1:6000, ThermoFisher Scientific, catalogue #A16047)
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bioRxiv - Cancer Biology 2023Quote: ... Cells were successively incubated with indicated primary antibodies in PBS/BSA 1% saponin 0.1% for 2 h at RT and with Alexa Fluor-conjugated secondary antibodies in PBS/BSA 1% saponin 0.1% for 1 h raised against mouse or rabbit immunoglobulins (Invitrogen). After washes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated with 1 µM Rhonox-M for 1 h or lysotracker deep red (Thermo Fisher Scientific L12492) according to the manufacturer’s instructions for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), and subsequently washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 594 Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer) and washed four times with PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were continued for another 3.5 h prior to addition of 1 µM NAO and/or 1 µM ReAsH-EDT2 reagent (Invitrogen). Cultures were incubated for 1 h at room temperature and washed with 1X BAL wash buffer per manufacturer kit instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated for 1 h at room temperature with Alexa-dye-conjugated secondary antibodies (1:1000; Invitrogen) in a blocking solution ...