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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Isolation of protein and nucleic acids from the same experimental sample was conducted using TRIzol™ (15596026; Invitrogen, Carlsbad, CA) and adapting the manufacture’s recommended procedure ...
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bioRxiv - Microbiology 2021Quote: ... Purified PCR products were sequenced by the Protein and Nucleic Acid Laboratory at Washington University using BigDye Terminator v3.1 Cycle Sequencing reagents (Life Technologies) using primers ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification of the lysed cells and exosome samples was performed using the bicinchoninic acid assay (BCA assay, Thermo Scientific). Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was used for protein purification by gravity-flow immobilized metal ion chromatography on nickel-nitrilotriacetic acid agarose (Ni-NTA Agarose, ThermoFisher). The column was pre-equilibrated with 20 mM Tris-HCl buffer containing 500 mM NaCl and 5 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration of the purified CYP168A1 was determined by bicinchoninic acid (BCA) assay (Pierce, Thermo Fisher Scientific, Waltham, MA) and the final concentration of the ferrous-CO protein was determined using UV-visible spectroscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein concentrations in cell lysates were measured using the Pierce™ bicinchoninic acid (BCA) assay kit (23225, Thermo Fisher). 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies ...
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bioRxiv - Physiology 2022Quote: ... Total protein abundance in tissue homogenates and lysates were determined in triplicates by the bicinchoninic acid method (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Amplicons were sequenced by the Washington University Protein and Nucleic Acid Laboratory using BigDye Terminator v3.1 Cycle Sequencing reagents (Life Technologies). Representative traces for all strains are available through the NCBI Trace Archive.
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bioRxiv - Neuroscience 2021Quote: ... Total protein content of the samples was measured with the bicinchoninic acid assay (kit from Pierce, Thermofisher Scientific, Göteborg, Sweden). Activity was expressed as mU/mg protein ...
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bioRxiv - Biochemistry 2020Quote: ... Radioactivity was measured using a liquid scintillation counter and protein concentrations were determined using bicinchoninic acid assays (Thermo Fisher Scientific). Results were calculated by correcting for total protein ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of protein in the crude adhesin fraction was determined using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were first purified by affinity chromatography on a Ni-nitrilotriacetic acid (Ni-NTA) agarose resin (Thermo fisher scientific). The column was washed with buffer A supplemented with 20 mM imidazole ...
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bioRxiv - Neuroscience 2022Quote: CeA punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations in the cell or tumor lysates were determined using the bicinchoninic acid (BCA) assay (Thermo Fisher Scientific, 23225). After protein concentration adjustment the samples (with equal amounts of protein ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected in 100 μL lysis buffer/well before determining total protein concentration by bicinchoninic acid (BCA) assay (#23225, ThermoFisher). According to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Purified samples were vacuum-dried and reconstituted in 0.1% (v/v) formic acid before before proceeding for absolute quantification of all proteins by LC-MS/MS analysis by Thermofisher Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer equipped with nano-LC Easy nLC 1200.
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bioRxiv - Immunology 2023Quote: ... All fractions were neutralized with Tris-HCl (0.1 M; pH 9.0) and their protein concentrations were determined by bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2024Quote: ... pH 8.2 by beating with 0.5 mm glass beads at 4°C and protein concentration was determined by bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Protein was reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Microbiology 2024Quote: ... The lysate concentration was determined from each sample by a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, USA). 40 -50µg of mitochondrial lysate were prepared in RIPA buffer with 1X Laemmili buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the supernatant for immunoprecipitation was measured via the bicinchoninic acid assay (Thermo Scientific, Rockford, IL, USA) (Walker ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Biophysics 2023Quote: ... 77,897 human protein sequences) using the Sequest search engines within the Proteome Discoverer platform (version 2.4, Thermo Scientific). The following search parameters were used ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... and non-essential amino acids (10 mM, glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, L-serine; Gibco). The pre-incubated viruses were then applied to Vero-E6 cell monolayers ...
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bioRxiv - Cancer Biology 2021Quote: ... cell were resuspended in 1x Annexin binding buffer (Thermo Fisher Scientific) and stained with AnnexinV BV421 (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody binding was detected by enhanced chemiluminescence (SuperSignal Dura, Thermo Scientific).
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bioRxiv - Immunology 2021Quote: High binding ELISA plates (Thermo Scientific, catalog no 44-2404-21) were coated at 0.5µg/mL with goat anti-mouse IgG2b (Southern Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... and nonspecific binding was blocked with Mouse IgG Isotype Control (Invitrogen) for 25 minutes at 4°C ...
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bioRxiv - Biophysics 2021Quote: Maleimide activated (Sulfhydryl-binding) stripe Plates (Cat. No. 15150, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed by direct dye binding (SYBR Green, Applied Biosystems). Primers were designed using Primer3 and blasted on the NIH website (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Microbiology 2022Quote: ... followed by washing with IgG binding buffer (Thermo Scientific, cat#21007). Antibodies were eluted with IgG Elution Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected on DIV15 in lysis/binding buffer (Invitrogen, A33562) and stored at -80 °C until mRNA extraction.
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bioRxiv - Developmental Biology 2022Quote: ... Binding of biotinylated Abs was detected by fluorochrome-conjugated streptavidin (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... Each well was washed with 1x Annexin binding buffer (ThermoFisher Scientific), and was centrifuged at 2,000 rpm (750g ...
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bioRxiv - Immunology 2019Quote: ... thymocytes were resuspended in Annexin V binding buffer (Thermo Fisher Scientific) with purified Annexin V (BioLegend ...
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bioRxiv - Immunology 2021Quote: To confirm RBD-specific binding of the selected nanobodies immunoplates (Nunc MaxiSorp ...
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bioRxiv - Immunology 2021Quote: ... Non-specific binding was blocked with 10% goat serum (Invitrogen, 50062Z) for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Nunc high-binding ELISA plates (Thermo Fisher Scientific; Waltham, MA, USA) were coated with 2 μg/ml of recombinant SARS-CoV-2 proteins (S1 and RBD-His proteins were produced in the lab and S1 +S2 ECD spike protein was purchased from Sino Biologicals ...
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bioRxiv - Immunology 2021Quote: ... nuclei were counterstained with the DNA binding dye Hoechst 33342 (Invitrogen). All images were captured with a Leica confocal system TCS SP5X inverted microscope ...
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bioRxiv - Microbiology 2022Quote: ... binding was detected with Alexa Fluor-647-labelled streptavidin (Molecular Probes) at 1 µg/ml for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were collected directly into lysis/binding buffer (Life Technologies), and mRNA was isolated using Dynabeads oligo (dT ...
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bioRxiv - Microbiology 2022Quote: ... assayed by dye binding (Qubit, Invitrogen / Thermo Fisher Scientific, Waltham, MA). Samples in were submitted to the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was revealed by the Enhanced Chemiluminescence System (Life Technologies) on X-Ray film (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 600 µl lysis binding solution concentrate (Invitrogen™, cat. no. AM1830) and 0.7% β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Binding was detected with Alexa Fluor-647-labelled streptavidin (Molecular Probes) at 1 μg/mL for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen, Sweden). Staining panels for monocytes and neutrophils are indicated in the supplementary tables 1 and 2 respectively ...
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bioRxiv - Immunology 2023Quote: ... TEP were aggregated in low binding 96-well plates (ThermoFisher, 174929), at a 8:1 ratio with fresh ETP sorted as described above ...
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bioRxiv - Genomics 2023Quote: ... Collected pellets were lysed using Lysis/Binding Buffer (Invitrogen Cat# 61012), and frozen at -80°C ...
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bioRxiv - Immunology 2023Quote: ... 96-well microtiter plates with high binding capacity (Thermo Fisher, USA) were coated overnight at 4°C with C.1086 Env gp140 protein from the NIH AIDS Reagent Program (ARP ...