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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer followed by PBS wash 15 min three times and then incubation for 2 h at room temperature with Alexa Fluor 594 conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher A-21207). Stained samples were then washed for 15 min three times in PBS ...
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bioRxiv - Immunology 2024Quote: ... 1:400 and for 1 hour at room temperature with the quaternary antibody: F(ab)’2-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were then immunolabeled with Alexa Fluor 647-conjugated donkey anti-mouse IgG (Yeasen, Shanghai) in PBS with 1:400 dilution for 2 h and the nuclear counterstain DAPI (Thermo Fisher Scientific) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with a goat anti-chicken Alexa 488 secondary antibody (#A11039, 1/500, Invitrogen, MA, US) and goat anti-mouse Alexa 555 (#A21422 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with PBS to remove unbound antibody and incubated for 2 h with Alexa Fluor-conjugated secondary antibodies (Invitrogen; 1:500), 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2024Quote: ... and E (SAgs, 2 μg/ml of each) for 1 h in serum-free AIM V™ medium (#12055-091; Gibco™). Unpulsed CFSE-stained Raji B cells were used as negative control (- SAgs) ...
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bioRxiv - Cell Biology 2024Quote: ... devices were washed twice with PBS and then incubated for 2 h with Alexa Fluor Goat anti-mouse 488 (1:250, Invitrogen, A-11029) secondary antibody and Hoechst (5 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Genomics 2022Quote: ... the purified gDNA was incubated at 37 °C for 1 h with RNase H (Thermo Fisher Scientific: 18021014) to remove hybridized RNA for RNase H-treated DRIP-seq library ...
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bioRxiv - Immunology 2020Quote: ... incubated for 1 h with secondary antibody Alexa Fluor 555 goat anti-mouse IgG (H+L) (A21422, ThermoFisher), washed with PBS ...
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bioRxiv - Biophysics 2021Quote: ... 1 mg/mL in 2:1 chloroform:methanol) is from Invitrogen (USA) and naphthopyrene from Sigma-Aldrich (St ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h at RT followed by Alexa Fluor 568-conjugated secondary antibodies (1:200 dilution, Invitrogen) for 45 min at RT ...
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bioRxiv - Immunology 2020Quote: ... Alexa-Fluor 555 conjugate secondary antibodies (Thermo Fisher Scientific, USA, 1:400, 1 h at 37°C) were used for labelling Spike ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 1 h with goat anti-rabbit AF647 (1:400; A-21224, Molecular Probes) or goat anti-mouse AF488 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... humidified chamber for 1 h with anti-mouse coupled to Alexa Fluor 488 (1:1000, Invitrogen, A11001), anti-rabbit coupled to Alexa Fluor 555 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody anti-rabbit A555 was incubated at room temperature for 1 h (1:500, Life Technologies). Nuclei were stained using DAPI (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by 1 h blocking and overnight incubation in either anti-chicken Alexa 488 (1:500, Invitrogen) or Cy5 anti-rabbit (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... osteoclasts were incubated for 1 h with Alexa Fluor 647-labelled phalloidin (1/1000, Life Technologies, #A22287), mouse anti-lys40 acetylated alpha tubulin (1/500 ...
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bioRxiv - Microbiology 2024Quote: ... This was followed by incubation of 1 h with goat anti-mouse DyeLight 800 (1:5000; Invitrogen) secondary antibody conjugated with a near fluorescent dye in 5% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reactions for the data corresponding to Figure 7 and Figure 7 – figure supplement 1 were carried out in 384-well plates on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
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bioRxiv - Neuroscience 2020Quote: ... at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL, Thermo Fisher Scientific). After washing 3 times with 0.3 M NaCl solution ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 50 μL were incubated at 4°C for 2 h with 40 μg of pre-equilibrated HisPur Ni–NTA magnetic beads (Thermo Scientific) in a final volume of 200 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded for 2 h at 4°C on a bi-directional rotator with 2 mM Ca2+ medium containing drugs 30 μM pancuronium (Fisher Scientific), or 38.9 μM curare (Sigma-Aldrich) ...