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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and quantitative RT-PCR performed using SuperScript II Reverse Transcriptase (Life Technologies) and then a Takyon SYBR Green PCR kit (Eurogentec ...
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bioRxiv - Pathology 2023Quote: ... using SuperScript™ IV first-strand synthesis system for RT-PCR (Invitrogen) with random hexamers (50 ng/μL) ...
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bioRxiv - Cell Biology 2022Quote: ... Conventional RT-PCR was performed by using AmpliTaq Gold® (Applied Biosystems) on C1000 Touch™ Thermal Cycler (Bio-Rad) ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR was performed using a QuantStudio3 Real-Time PCR system (ThermoFisher). Reactions consisted of 5 μl PowerUpTM SYBRTM Green Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were assayed on a 7500 RT-PCR system (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR reactions were performed in a SimpliAmp Thermal Cycler (Applied Biosystems) following the cycling conditions described in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was performed using platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA) with human-specific primers for miR-214 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative RT–PCR was performed using iQ SybrGreen Supermix (4368708, Applied Biosystems) on the Bio-Rad CFX96 C1000 Touch Thermal Cycler and analyzed.
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bioRxiv - Cell Biology 2023Quote: We used TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems) as described previously (19,24 ...
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bioRxiv - Microbiology 2024Quote: ... using the AgPath-ID One-Step RT-PCR kit (Thermo Fisher Scientific) with target-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed using the ViiA7 QRT-PCR machine (Applied Biosystems). Relative gene expression was calculated using ΔCT method normalised to human B2M housekeeping gene (See Table 5).
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bioRxiv - Microbiology 2024Quote: ... Real-time RT-PCR was performed using a StepOnePlus thermocycler (Applied Biosystems). The final volume of the reaction cocktail was 20 μL ...
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bioRxiv - Cell Biology 2021Quote: ... Relative gene expression was determined by quantitative real time PCR (q-RT-PCR) using the ViiA 7 Real Time PCR system (Applied Biosystems, USA). The reaction mixture consisted of 0.75μL 500nM forward primer ...
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bioRxiv - Immunology 2021Quote: RNA samples were quantified by quantitative reverse transcription-PCR (qPT-PCR) using Superscript III one step RT-PCR system with Platinum Tag Polymerase (Invitrogen, Darmstadt, Germany). Primer and probe sequences for mRNA encoding the SARS-CoV-2 E gene (Corman et al. ...
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bioRxiv - Immunology 2022Quote: Real-time RT-PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (ThermoFisher Scientific #4368708). Primers for HA (forward 5’-AAACTCTTCGCGGTCTTTCCA-3’ ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... using commercial buffer and primer sets for the amplification of ACE2 (Hs01085333m1) and TMPRSS2 (Hs01122322m1) transcripts (Thermo Fisher Scientific, Darmstadt, Germany). We analysed the transcript of TATA box binding protein (TBP ...
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bioRxiv - Evolutionary Biology 2022Quote: The amplified fragments obtained with the Arsenophonus primer set were sequenced with the BigDye™ Terminator v1.1 Cycle Sequencing Kit (ThermoFisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Syber green-based qPCR was performed using primer sets (Table S1) with 50 ng of cDNA from each sample in the QuanStudio-6 qPCR system (Applied Biosystems) with thermocycle condition ...
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bioRxiv - Cell Biology 2022Quote: ... with gene-specific primer sets (Supplemental Table 2) using the Platinum SYBR Green qPCR SuperMix-UDG with ROX (Invitrogen, #11733-046) under the following reaction conditions ...
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bioRxiv - Microbiology 2024Quote: ... gene for the internal normalization primers-probe (VIC) set (VIC-MGB: assay ID Hs03405707_g1; TaqMan Gene Expression Assay, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Microbiology 2023Quote: ... Both primer sets were used at a final concentration of 0.2 µM with Phusion High-Fidelity DNA polymerase (Thermo Scientific, USA). According to Illumina (San Diego ...
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bioRxiv - Cell Biology 2023Quote: ... Technical triplicate reactions were set up for each sample and target primer in MicroAmp Optical 384-well Reaction Plates (Life Technologies), and reactions were run and analyzed on a Quant Studio 12K Flex Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription quantitative PCR (RT-qPCR) was performed using a QuantStudio 3 realtime PCR system (Thermo Fisher Scientific) with SYBR Green JumpStart Taq ReadyMix (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Q-PCR amplifications were performed on a Applied Biosystems Step One Plus RT PCR system (Thermo Fisher Scientific) at 95 °C for 10min ...
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bioRxiv - Cell Biology 2019Quote: ... The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). qPCR results were analyzed using the ΔΔCt method relative to the mean of housekeeping genes (Rps12 ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using a TaqMan One-Step RT-PCR Master Mix Reagents Kit (ThermoFisher, cat. # 4309169) and primers for a genomic locus (b-actin ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... The quantitative RT-PCR for indicated genes was performed in TaqMan Universal PCR Master Mix (Applied Biosystems, USA). Quantitation of mRNAs was performed using Applied Biosystems™ TaqMan™ Gene Expression Assays according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The RT-PCR was performed using first-strand cDNA with TaqMan Fast Universal PCR Master Mix (ThermoFisher Scientific). TaqMan primer-probes were obtained from ThermoFisher Scientific (BSG Hs00936295_m1 ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time RT-PCR was performed with the EXPRESSS One-Step SuperScript qRT-PCR kit (Thermo Fisher Scientific) using gene-specific primers (Supplementary Table 2 ...
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bioRxiv - Pathology 2021Quote: ... The PCR mastermix was prepared using the AgPath-ID One-step RT-PCR kit (Thermo Fisher Scientific, USA) in a volume of 10 µl including 0.5 µl of β-Actin-mix2-HEX as internal control and 2.5 µl of extracted RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time RT-PCR (qRT-PCR) was performed using 0.01-0.05μg cDNA with SYBR Green mastermix (Applied Biosystems) in an Applied Biosystems thermocycler ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was then performed using Phusion Green Hot Start II High-Fidelity PCR Master Mix (Thermo Scientific) and 20-100 ng cDNA ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription PCR was performed on extracted RNA using SuperScript™ IV One-step RT-PCR System (Invitrogen). The sequence-specific primers were used to generate an amplicon of 905 bp covering the D614G region ...
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bioRxiv - Immunology 2022Quote: ... and analyzed by RT-PCR with a Step One Real-time PCR system as described above (Applied Biosystems).
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bioRxiv - Molecular Biology 2020Quote: ... The RT-PCR was performed using a thermo cycler (ABI 7500 Real-Time PCR, Thermo Fisher Scientific, USA) at 95 °C for 10 min ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was performed by quantitative real time RT-PCR using Power SYBR Green PCR Mix (Applied Biosystems) using StepOnePlus detection system (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Real-time RT-PCR experiments were performed using a Power SYBR™ Green PCR Master Mix (Thermo Fisher). The following primers were used for the qPCR reactions ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed with the TaqPath™ COVID□19 CE□IVD RT□PCR Kit (Thermo Fisher Scientific) to obtain mean cycle threshold (Ct ...
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bioRxiv - Biochemistry 2024Quote: ... these reactions were monitored by real-time PCR on a StepOne RT-PCR instrument (Applied Biosystems, Waltham, MA) and terminated during mid-log amplification (17 or 18 cycles) ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed in 48-well plates using the Step-One Real-Time PCR System (Applied Biosystems). Relative changes in gene expression between control and treated samples were determined using the 2 (-Delta Delta CT ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in a QuantStudio 7/12k Flex System (Applied Biosystems) with the following primers Crbn.F 5’-TGAAATGGAAGTTGAAGACCAAGATAG-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Both cDNA synthesis and PCR amplification were done with the SuperScript III one-step RT-PCR system (Invitrogen). TaqMan probes for the housekeeping genes ACTB (Hs01060665_g1 ...
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bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR reactions were carried out on a Real-time PCR machine (QuantStudio 5 by Applied Biosystems), according to a protocol from the Bimake SYBY Green Master Mix qPCR Kit (B21402) ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2023Quote: ... Real time (RT)-PCR was performed using Fast SYBR Green PCR Master Mix reagent (Thermo Fisher Scientific, USA) with the following reverse and forward primers ...
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bioRxiv - Genomics 2023Quote: ... and cDNA synthesis and PCR were performed using SuperScript IV One-Step RT-PCR System (Thermo Fisher Scientific) with primers designed at 3’UTR of bovine ZDBF2 according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Thermo Fisher) and the Applied BiosystemsTM ViiA Real-Time PCR Systems by relative quantitative RT-PCR (Thermo Fisher Scientific ...