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Citations for Thermo Fisher :
1051 - 1100 of 7168 citations for 6 HYDROXY 7 METHYLPURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Genetics 2021Quote: ... treated wild-type C57BL/6 and 12-week-old Rad1 CKO mice were subjected to phosphopeptide enrichment and 6-plex TMT (ThermoFisher) labeling (Sims et al. ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Immunology 2024Quote: ... primary Rhinolophus ferrumequinum bat cells were seeded in 6-well plate at a density of 6×105 cells/well with DMEM containing 10% FBS (Gibco). After 12 h ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was transfected into the Sf9 insect cells seeded in 6-well plates at 0.5 × 106 cells/ml using Fugene 6 reagent (Invitrogen protocol). At 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Immunology 2024Quote: ... IL-6 levels in the supernatant and plasma were determined using a high sensitivity IL-6 ELISA Kit (BMS603HS, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... or 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 10 mM Laurdan (6-Dodecanoyl-2-Dimethylaminonapthalene, Invitrogen) stock solution was prepared in 100% dimethylformamide (DMF ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (QuantStudio 6, Applied Biosystems, Waltham, MA) was used to measure gene expression levels and normalized to the housekeeping gene hypoxanthine guanine phosphoribosyl transferase (Hprt) ...
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bioRxiv - Cell Biology 2022Quote: ... on a QuantStudio 6 Flex instrument (Applied Biosystems) in a 96 well format ...
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bioRxiv - Neuroscience 2019Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) staining was added to visualize nuclei.
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bioRxiv - Immunology 2019Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well plate ...
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bioRxiv - Microbiology 2019Quote: ... on a QuantStudio 6 Flex machin (Applied Biosystems). Genome equivalent (GE ...
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bioRxiv - Immunology 2020Quote: ... using a Quant Studio Flex 6 (Applied Biosystems). Samples were run in triplicate wells in a 96-well or 384 well plate ...
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bioRxiv - Microbiology 2019Quote: ... and 3-6 μl Lipofectamin 2000 (Life Technologies). See the Supplemental Information for detailed description.
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bioRxiv - Bioengineering 2022Quote: ... 6-diamidino-2-phenylindole (Thermo Fisher Scientific, D1306) for 45 minutes at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to Essential 6 media (ThermoFisher) and was replaced daily for 4 days ...
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bioRxiv - Bioengineering 2021Quote: ... 6 mL FACS Rinse (Thermo Fisher, BD 340346), 6 mL 80% ethanol ...
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bioRxiv - Bioengineering 2020Quote: ... 6-well culture plates (Corning; Thermo Fisher Scientific), and overlaid with glass coverslips to aid in tissue adherence ...
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bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
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bioRxiv - Biochemistry 2020Quote: ... 6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Molecular Probes).
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bioRxiv - Biophysics 2021Quote: ... 6 mM L-glutamine (Thermo Fisher Scientific, USA), 0.2 % (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6- diamidino-2-phenylindole (DAPI; Invitrogen D1306) was added during the secondary antibody incubation at a concentration of 700 ng/ml ...
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bioRxiv - Plant Biology 2021Quote: ... Gel (Novex TBE 6%, Invitrogen, cat. no. EC6265BOX) selection and purification was performed to enrich libraries containing siRNA fragments ranging from 20 to 30 nt ...
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bioRxiv - Genomics 2020Quote: ... in a QuantStudio™ 6 system (Applied Biosystems). See Supplementary Table 1 for primer sequences used in this study ...
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bioRxiv - Microbiology 2019Quote: ... For TMT 6-plex labeling (Thermo Fisher Scientific), digested peptides were treated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 6 diamidino-2-phenylindole (DAPI) (ThermoFisher scientific, 10116287) and mounted with Fluoromount-G (Cambridge Bioscience) ...
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bioRxiv - Cancer Biology 2021Quote: ... or QuantStudio-6 Real-Time System (Applied Biosystems). Power SYBR Green Cells-to-Ct kit was used to perform quantitative PCR on 10,000 cells sorted from tumors ...
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bioRxiv - Neuroscience 2022Quote: ... in Essential 6 medium (Thermo Fisher Scientific, A1516401) supplemented with two SMAD pathway inhibitors - dorsomorphin (2.5 μM ...
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bioRxiv - Cell Biology 2022Quote: ... 6-diamidine-2′-phenylindole dihydrochloride (Thermo Fisher Scientific) was added for nuclear counterstaining ...
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bioRxiv - Neuroscience 2022Quote: ... and 6 μl of NFW (Ambion™, AM9937). The PCR reaction was carried out using the following conditions ...
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bioRxiv - Biophysics 2022Quote: ... 6 μL PageRulerPlus Prestained Protein Ladder (ThermoFisher Scientific) was loaded to monitor the progress of the SDS-PAGE and to estimate the approximate size of the protein after staining the gel ...
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bioRxiv - Bioengineering 2022Quote: ... 2-6 mL (Thermo Scientific, catalog no. 88516), and the final protein concentration was measured via A280 absorbance.
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bioRxiv - Neuroscience 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) (Invitrogen, 1:10000) and then washed before mounting with FluorSave (Calbiochem).
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bioRxiv - Neuroscience 2021Quote: ... propanal (ACROS Organics, 1%, CAS# 123-38-6), and 1,4-diaminobutane (ACROS Organics ...
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bioRxiv - Neuroscience 2019Quote: ... counterstained with DAPI (4’,6-diamidino-phenylindole, Invitrogen) for 45 min (both steps at room temperature) ...
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bioRxiv - Pathology 2021Quote: ... qPCR was performed with QuantStadio 6 (Thermo Fisher). Taqman PCR probes (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... A 6% TBE polyacrylamide gel (Novex, Thermo Scientific) was pre-run at 10 V for 10 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... 6-diamidino-2-phenylindole dihydrochloride (DAPI, Thermofisher Scientific). The mountant was allowed to cure overnight and coverslips were analysed on an Olympus FV3000 confocal microscope.
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bioRxiv - Biochemistry 2020Quote: ... 6 µL of NativePAGE sample buffer (Invitrogen, BN20041) was added and 10 µL of sample was run on precast 3-12% NativePAGE gels (Invitrogen ...