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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 2 μl of 1 μM Qdot 655 Streptavidin Conjugate (ThermoFisher) was incubated with 2 μl of 100 nM biotinylated kinesin-543 and 6 μl of Buffer 1 on ice for 1 hour prior to experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2 mM glutamine and 1% penicillin/streptomycin (Gibco) and 5% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:10000, Thermo Fisher Scientific). Semi-quantitative methods were utilized for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... or rabbit ATF 2 antibody (1:100, ThermoFisher, #MA5-32022) overnight at 4°C and then with anti-rabbit 488 (1:200 ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM L-glutamine,1% (v/v) penicillin/streptomycin(Gibco)) and 2 million cells were seeded in 6cm collagen I-coated dish (BD BioCoat) ...
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bioRxiv - Cell Biology 2024Quote: ... and 4′,6-diamidino-2-phenylendole (DAPI; 1:5000, Invitrogen) or Hoechst (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... 1 volume of PBS with 2% FBS (Gibco, A38400-01), the transcription/translation inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... brains were washed trice with PBS for 5 min and free amines were anchored with 0.1mg/mL succinimidyl ester of 6-((Acryloyl)amino)hexanoic acid (Acryloyl-X, SE, Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: Cardiomyocytes were loaded with 2 μM Fura-2 (Thermo Fisher, F1201) in Tyrode’s solution for 15 min ...
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 2 μM fura-2 AM (ThermoFisher Scientific) in BSS for one hour at 37 °C and rinsed twice with BSS (Gibson et al ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM GlutaMax supplemented with 2% (wt/vol) B27 (GIBCO) and 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Methanol (CH3OH) 99.8% and pyridine 99.5% were purchased from Acros Organics (Geel, Belgium). 1,2-Distearoyl-sn-Glycero-3-Phosphocholine (DSPC ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μg of anti-rabbit IgG was incubated with 8 μg of Alexa Fluor 750 N-Succinimidyl Ester (Thermo Fisher Scientific) in 100 mM NaHCO3 (pH 8.3 ...
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bioRxiv - Developmental Biology 2019Quote: ... 5-ethyl-20-deoxyuridine (EdU; Invitrogen E10187) was injected intraperitoneally and detected as described in (Comai et al. ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 M N-ethyl maleimide (ThermoFisher, #23030) and protease inhibitor (Cell Signalling Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were initially incubated for 30 minutes at room temperature with 5 μM Fura-2-AM and 0.005% pluronic acid (ThermoFisher) in HEPES-aCSF solution (125 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... we prepared a 2-fold dilution series of a 1x “stimulation” solution containing 1x essential amino acids (Invitrogen, 11130051), 1x non-essential amino acids (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Physiology 2019Quote: ... Cells were incubated with fura-2AM (2 μM) (Molecular ProbesTM) in SBS containing 0.01% pluronic acid (Thermo Fisher Scientific) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were prepared for analysis by staining with 2 x SYBR Green I nucleic acid gel stain (Life Technologies) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... diluted 2 times with 0.1 % formic acid and desalted on strong cation exchange micro-tips (StageTips, Thermo Fisher scientific) as described72 ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 ul NET-2 and extracted with an equal volume of acid-phenol:chloroform (Invitrogen AM9722). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... by recording the increase in the absorbance at 405 nm after the reaction of reduced glutathione with 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) for 2.5 min at 15-s intervals using a Varioskan Flash (Thermo Fisher). GSSG was quantified after derivatization of GSH with 2-vinylpyridine ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 22 cm long ...
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bioRxiv - Bioengineering 2023Quote: ... 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB, aka Ellman’s Reagent) and DL-dithiothreitol (DTT) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 35 cm long ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Neuroscience 2021Quote: ... seeded at 1.5 x 105 neurons per coverslip in Neuron Medium (8 % FBS, 2 % B-27, [Gibco, 17504-044], Penicillin [200 U/mL]/Streptomycin [200 μg/mL] [Gibco, 15140-130] ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-B16 tumor spheroids were transferred to Lab-Tek II cover-glass-bottomed 8-chamber slides and imaged in HBSS supplemented with 2% FBS (all from Invitrogen) using a 63×/1.4 NA oil-immersion objective.
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Cell Biology 2020Quote: ... pALN25: GFP-BUB1-T609A) and selected with 2 mg/ml (HeLa) or 8 mg/ml (K-562) Blasticidin (Life Technologies) (Cheeseman and Desai ...
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bioRxiv - Molecular Biology 2023Quote: Proliferating C2C12 myoblasts were transduced with lentivirus in the presence of 8 µg/ml polybrene and selected with 2 µg/ml puromycin (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... and submerged in cutting buffer (8 mM Na2HPO4.2H2O, 2 mM KH2PO4, pH = 7.4) in the Micron HV650 (Thermo Fisher Scientific). Coronal sections of 60µm were cut using a frequency of 70Hz ...
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bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of cRNA was used for reverse transcription with 2 pmoles of a 16S rRNA-specific primer (Supplementary Table 8) and SuperScript II (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Immunology 2022Quote: ... 1 - 2 μg ml-1 poly(dA:dT) (InvivoGen) (transfected with Lipofectamine 2000, Invitrogen), 100 µM imiquimod (R837 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and 4′,6-diamidino-2-phenylindole (DAPI, 1:1,000, Life Technologies). Following several washes ...
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bioRxiv - Neuroscience 2023Quote: ... containing 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Cultured neurons were fed with 250 μl NB+ media on days in vitro (DIV ...
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bioRxiv - Neuroscience 2022Quote: ... IL-6 (1:4-1:2 dilution, #KMC0061; ThermoFisher Scientific, Waltham, MA, USA), and NfL (1:4 dilution ...