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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 7 Benzothiazolol 2 1 1 dimethylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... HeLa (CCL-2, ATCC), COS-1 (CRL-1650, ATCC) and L-929 (CCL-1, ATCC) cells were maintained in DMEM (11995073, Gibco) supplemented with 1% Penicillin-Streptomycin (15140122 ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Neuroscience 2023Quote: ... The base medium was gradually transitioned to N2/B27 medium (Neurobasal medium, 2% B27 supplement, 1% N2 supplement, 1% Glutamax, (ThermoFisher)) in 25% increments ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Buffy coat was mixed 1:2 with PBS and added onto a Ficoll gradient in a 3:1 ratio (Invitrogen). This was centrifuged at 2100 rpm for 25 minutes at RT (w/o brakes) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in TBS for 10 min each before incubation for 1-2 h at RT with the following Alexa Fluor secondary antibodies made in goat (1:500; Invitrogen): anti-rabbit Alexa 488 (A11008) ...
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bioRxiv - Neuroscience 2023Quote: ... and re-plated on Matrigel-coated dishes in NPC Medium (DMEM/F12, 2% B27, 1% N2, 1% Pen-strep; all from Gibco) supplemented with 20 ng/mL bFGF (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in PBS for 1 hour at room temperature and incubated 2 hours at room temperature with the appropriated Alexa488 (1/400, Invitrogen), Alexa594 (1/400 ...
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bioRxiv - Cell Biology 2023Quote: ... Counter-staining cell nuclei with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 μg ml−1 in PBS) and F-actin with Alexa Fluor dye conjugated phalloidin (Invitrogen) was also done at this step ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... the purified DNA in 1 ml NaHCO3 (pH 8.3) was incubated with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 mins at 20-22°C ...
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bioRxiv - Cancer Biology 2023Quote: BE(2)-C (ATCC, CRL-2268) cells were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Developmental Biology 2023Quote: ... The culture medium consisted of a 1:1 mixture of DMEM with 2 mM l-glutamine and F12 (Ham’s Nutrient Mix) (both from Life Technologies) and was supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were suspended in culture medium (Neurobasal medium, 2% B27, 1% GlutaMAX™, GIBCO, 1% antibiotic/antimycotic (ThermoFisher Scientific)) supplemented with mouse NGF (50ng/μl ...
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bioRxiv - Immunology 2023Quote: The whole blood samples were 1:1 diluted with 2% fetal bovine serum (FBS) (Hyclone, SH30406.05) in phosphate buffered saline (PBS) (Invitrogen, C10010500BT) and subjected to Ficoll (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid (EV or HLA-A2/Kb; 1 µg/µL) complex at 2:1 ratio prepared in Opti-MEM (Gibco, UK) for 18 hours in the presence or absence of 100 ng/mL type I IFNα (PBL Assay Science ...
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bioRxiv - Microbiology 2024Quote: ... the PBS or virus dilution in PBS was aspirated and 3 ml of overlay consisting of 1:1 2 x DMEM (DMEM high glucose, no sodium bicarbonate buffer powder [Gibco # 12-100-046] in 500 mL of RNase-free water ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures on feeder layers were performed on Mitomycin C-treated MEF as described above with passaging every 7 days using 1 mg/mL collagenase IV in DMEM/F12 (Life technologies) the feeder-free culture was performed on Geltrex™ (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... Then to stain the cells we used antibodies against LFA-1 (clone I21/7) and 4G10 with Alexa Fluor 568–conjugated phalloidin (Life Technologies) and DAPI ...
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bioRxiv - Biochemistry 2019Quote: ... The valve then switches to engage the passive split such that a majority of LC effluent is diverted to waste prior to the third dimension PC. 13 RP-SAX-RP fractions were analyzed. Either an LTQ Velos (Figs. 1, 7) or Q-Exactive HF (Thermo Scientific) machine was used to acquire MS/MS spectra in data-dependent mode as described (Zhang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Neuroscience 2020Quote: Adrenal glands and brain extracts were collected from P0-1 Syt-7 WT and KO mice and lysed in RIPA buffer supplemented with Protease Inhibitor Cocktail (Invitrogen, 89900). The supernatants were collected and protein concentrations were estimated by using the BCA Protein Assay Kit (Pierce 23227 ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... the percentage of apoptotic astrocytes was evaluated using CellEvent Caspase-3/7 Green Detection Reagent (1:250; Thermo Fisher, cat. #C10423) added directly to the medium ...
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bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
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bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
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bioRxiv - Evolutionary Biology 2021Quote: For ethanol preference assays, experimental substrates were 1% agarose and contained 75mM sucrose and increasing concentrations (3%, 5%, and 7%) of ethanol (ThermoFisher #BP2818); control substrates were 1% agarose and contained 75mM sucrose.
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Biophysics 2021Quote: ... and DRAQ-7 (Thermofisher) was administered at 1:100 ratio to every sample droplet and the Petri dish was stored at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... KLRG1 (PeCyanine 7, Invitrogen, FITC ...
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bioRxiv - Bioengineering 2020Quote: ... 7-AAD stain (Invitrogen) was added to each sample (2.5 μL per well in the 96-well plate ...