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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Wild-type human HCAR2 was cloned into pFastBac vector (Gibco) with N-terminal hemagglutinin (HA ...
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bioRxiv - Physiology 2023Quote: ... and Collagenase type I 500µg/ml (Thermo Fisher Scientific, 17018029). Liver lobes were collected in Washing Medium (William’s medium (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Tetravalent recombinant (wild-type) streptavidin was procured from Thermo Scientific. Plasmids encoding SBD2 (T369C/S451C ...
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bioRxiv - Neuroscience 2024Quote: ... Both types of cultures were maintained in Neurobasal medium (Invitrogen), penicillin/streptomycin and 2% B27 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... of which one type had removable wells (177402, Thermo Fisher). These were mounted on glass cover slides using anti-fade fluorescence mounting medium (ab104135 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Biochemistry 2024Quote: ... permeabilized with 0.2% Triton X-100 in PBS for 5 mins at room temperature and incubated in primary ZO-1 antibody (ZO-1 polyclonal rabbit antibody, Invitrogen #61-7300, 1:2,000) diluted into blocking buffer for 2 hrs at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... dissected wild-type neural folds that had been incubated for 30 minutes at 37°C in a 1:500 Calcein-AM (Invitrogen, C3099)/DMEM (supplemented with Penicillin-Streptomycin and N-2 ...
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bioRxiv - Bioengineering 2020Quote: ... and wild type (WT) fibroblast cells (Tissue Culture Solutions Cell Works, UK) were cultured in DMEM supplemented with 1% Glutamax (Invitrogen™), 1% penicillin and streptomycin solution (Gibco® ...
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bioRxiv - Microbiology 2021Quote: ... wells of 96 wells plate were coated with 100 µL of 40 µg.mL-1 rat tail type I collagen in sterile PBS (Life Technologies, NY) at 4°C for 18 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The ganglia were washed twice by pelleting at 800g for 3 min and resuspending in 5 mL ‘HBSS+H’ then enzymatically digested by resuspension in 1 mL 37°C HBSS supplemented with 15 μL 20 mg/mL collagenase type IV (Thermo Fisher). After 1 hour incubation at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... then placed in fresh ice-cold HBSS and cut into small pieces to be digested in prewarmed HBSS with 1 mg/ml Collagenase Type 2 (Life Technologies) at 37 °C for 5 min with gentle agitation ...
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bioRxiv - Microbiology 2022Quote: ... growth medium (200 µL, 24 h), bacterial cultures (200 µL, 24 h of growth)) and pre-cleaned type 1 glass vials (VOA; Thermo Scientific) were chilled on ice for 10 min ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were transfected with 1 μg of pCAGGS plasmid encoding the NL09 wild-type or escape mutant HA proteins using Lipofectamine (Invitrogen, USA) and Plus reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: Jurkat-wild-type (WT) cells were washed twice with PBS and then stained with the calcium sensor dye Indo-1 AM (Invitrogen I1223), at 37 °C and 5 % CO2 for 30 mins as described (Lo et al. ...
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bioRxiv - Immunology 2024Quote: Lungs were perfused with PBS-EDTA (0.5 mM) and digested in RPMI-1640 medium containing 1 mg/mL collagenase type IV (Life Technologies/ Thermofisher) and 30 µg/mL DNase I (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... stem cell cultures were washed in sterile Dulbecco’s phosphate buffered saline (DPBS) and incubated at 37°C in a Collagenase type IV/Dispase solution (1 mg/mL each, Thermo Fisher) until colonies detached from the feeder layer (typically after 15’–20’) ...
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bioRxiv - Bioengineering 2023Quote: ... The dermal layers of the skin were separated using forceps and further subjected to incubation with type I collagenase (1 mg/ml Gibco, USA) for 2 hours at 37 degrees ...
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bioRxiv - Cell Biology 2023Quote: ... and the EDL was immediately incubated in plating medium (DMEM + 10% horse serum + 2% Penicillin/Streptomycin + 1% HEPES) containing type I collagenase (2.6 mg/mL; Gibco, 17100-017) in a 37°C shaking water bath for 53 minutes ...
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bioRxiv - Cell Biology 2024Quote: For the generation of stable cell lines U2OS FlpIn TRex cells were transfected with pcDNA5/FRT/TO-SNX10-EGFP wild type or mutant constructs (Table 1) using Lipofectamine 2000 (Invitrogen, #11668019). Positively transfected cells were selected with hygromycin B (EMD Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... and vascular endothelial growth factor receptor 2 (VEGFR-2, Catalog No. PA5-16487, Thermo Fisher Scientific, Waltham, MA). For sections being stained for a target antigen ...
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bioRxiv - Immunology 2020Quote: ... PGT145 and PGT121 receptors (in a form of IgM) were grown in advanced Dulbecco’s modified Eagle’s medium (DMEM) (Gibco), supplemented with 10 % fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: The chemicals (S2 Table) used for the deorphanization of receptors were obtained from Acros Organics (Morris, NJ, USA), Alfa Aesar (Ward Hill ...
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bioRxiv - Neuroscience 2024Quote: A synthetic DNA geneblock for the human PAC1R-null receptor sequence (PAC1R) was designed and ordered (Life Technologies) based on the NCBI protein data bank entry “NP_001109.2” ...
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bioRxiv - Microbiology 2023Quote: ... Soluble EFNB2-Fc was constructed by cloning the receptor binding domain (a.a. I28 to G165) of EFNB2 into the NheI-BamHI sites of pcDNA3.1(+) (Invitrogen) with a N-terminal CD5 leader (MPMGSLQPLATLYLLGMLVASVLA ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μg of RNA was reverse transcribed using either 1 μg/ml random primers and Superscript II (Life Technologies, Benicia, CA) or the miSCRIPT II RT kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed using 1 μg/ml random primers and SuperScript II reverse transcriptase (Life Technologies, Benicia, CA). Intron-spanning oligo primers to ROBO1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed using 1 μg/ml random primers and SuperScript II reverse transcriptase (Life Technologies, Benicia, CA). Primers to ARF6 and 18s were designed using the Roche Universal Probe Library assay design center and qPCR was performed using Quanta PerfeCTa Supermix ...
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bioRxiv - Cell Biology 2023Quote: ... and fibroblasts were cultured for 2 days in DMEM/F12 (1/1) supplemented with 10 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France) and 1% Penicillin/Streptomycin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... were seeded on the top of the membrane and cultured for 3 days in DMEM/F12 (1/1) supplemented with 5 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France), 0.2 ng/ml EGF (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ml of collagenase mix digestion solution (collagenase II + IV mixed at 1:1 ratio at 2 mg/ml -Thermo Fisher Scientific) was added to the tissue and incubated horizontally for 20 min at 37°C in a shaking incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were developed with a mouse polyclonal anti-SmHDAC8 antibody (1/1000) or mouse polyclonal anti-SmRho1 antibody (1/1000) and peroxidase coupled anti-mouse secondary antibody (1/50 000; Invitrogen). Detection was carried out by chemiluminescence using SuperSignal™ West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and finally stained with the anti-sHP1γ antibody (1:250) and Alexa Fluor 488 anti-rabbit secondary antibody (1:500, Invitrogen). Coverslips were mounted in VectaShield mounting media with DAPI for immunofluorescence ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated surface GLT-1 transporters were identified by using anti-GLT primary antibody (1:500, Alomone) or anti-V5 primary antibody (1:2000, Invitrogen) and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and anti-VE-cadherin antibody (1:100, Cell Signalling) followed by anti-rabbit Alexa 488 secondary antibody (1:1000, Invitrogen) to mark EC junctions.
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bioRxiv - Neuroscience 2021Quote: ... Blot sections were probed overnight at 4°C 1:5000 with the ATXN1 antibody 11750 (Servadio et al., 1995) or 1:2500 with H1 antibody (Invitrogen) diluted in 5% milk PBST ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 hour incubation with Alexa Fluor 647-conjugated secondary antibody (donkey anti guinea pig, 1:1000) or Cy3-conjugated secondary antibody (goat anti mouse,1:1000, Invitrogen) respectively.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... protein was detected using a specific antibody (1:1500, Sinobiological) and an Alexa Fluor 488 anti-rabbit secondary antibody (1:200, Invitrogen). The nucleus was labeled using DAPI (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Tyrosine hydroxylase immunoreactions (primary antibody: Chicken anti-TH, 1:500 Abcam; secondary antibody: Alexa Fluor 488 1:1000, Life Technologies) were carried out in 3 or more striatal and at least 1 nigral section ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... Samples were probed with a primary antibody against MCT4 (1:1000; AB3314P, Merck Millipore and AlexaFluor488 secondary antibody (1:2000; A11008, Invitrogen). Nuclei were stained with 4,6-diamidino-2-phenylindole (1:50,000 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a NPC1 primary antibody (1:70) followed by a DY650 secondary antibody (1:400) and mounted with PermaFluor Aqueous Mounting medium (ThermoFisher). Imaging was performed with a LSM800 confocal microscope (AxioObserver Z1 ...
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bioRxiv - Microbiology 2022Quote: ... Spike protein was detected by primary antibody (1:1500, Sinobiological) followed by Alexa Fluor 647 anti-rabbit secondary antibody (1:1000, Invitrogen). The nucleus was labelled using DAPI (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit monoclonal antibodies against VEGFR2 (1:200, clone 55b11, Cell Signalling) and rabbit polyclonal antibodies against COL15A1 (1:200, Invitrogen) was performed ...
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bioRxiv - Microbiology 2024Quote: ... The IMC protein GAP45 was labeled using the anti-GAP45 rabbit antibody (1:5000) (a kind gift from Julian Rayner) and goat anti-rabbit AlexaFluor 488 antibody (1:500, Invitrogen). Alfa-tubulin was labeled with an anti-alfa tubulin mouse antibody (1.500 ...
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bioRxiv - Microbiology 2024Quote: ... primary antibody for 1 h followed by Alexa Fluor 647 goat anti-rabbit secondary antibody (1:1000; Cat. A21245, Invitrogen) for 1 h to label the CCVs ...
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bioRxiv - Microbiology 2023Quote: ... 96-well plates were blocked with 2% BSA in PBS for 30 minutes and then stained with a 1:500 dilution of mouse anti-RhCMV pp65b antibody clone (VGTI Monoclonal Antibody Core) in PBS for 1 hour followed by a 1:1000 dilution of Alexa488-conjugated anti-mouse antibodies (Invitrogen) and 3 µM DAPI (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated for 45 minutes in secondary antibody solutions with AF647 secondary antibodies (anti-mouse, 1:1000, A31571, Invitrogen or anti-rabbit, 1:1000, A21245, Invitrogen) or Alexa Fluor 555 secondary antibodies (anti-rabbit ...