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Citations for Thermo Fisher :
901 - 950 of 6994 citations for SIRPA Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: HEK293 cells were transfected with 3 μg of linearised plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000001) in wells of a 6-well plate ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified using HisPur Ni-NTA Resin (Thermo Fisher). Resin-bound proteins were washed (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and templated using the Ion PI Hi-Q Chef kit (Thermo Fisher). The libraries were sequenced on an Ion Proton system (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Hi-Q reagents and PI™ chip kit V3 (ThermoFisher, Waltham, USA) and the sequencing was performed for 300 flows ...
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bioRxiv - Synthetic Biology 2019Quote: ... Dual immunogold labeling was conducted with 6x-His Tag Monoclonal Antibody (Invitrogen) and the HA Tag Polyclonal Antibody as primary antibodies and an anti-mouse IgG-gold (40 nm ...
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bioRxiv - Genetics 2019Quote: To the amplification products were added formamide (Hi-Di Formamide, Applied Biosystems) and the GeneScan 500 molecular weight standard labeled with the fluorochrome LIZ ™ (orange fluorescence ...
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bioRxiv - Cancer Biology 2020Quote: ... respectively supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco). Co-cultures of JeKo-1/REC-1 with MS-5 and HS-5 at a 10:1 ratio was maintained under the same conditions as for MS-5 or HS-5 cells alone ...
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bioRxiv - Plant Biology 2021Quote: ... using the Ion PI Hi-Q OT2 200 Kit (ionTorrent, Life Technologies).
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bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
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bioRxiv - Genomics 2020Quote: All splicing reporters were cloned into pAC-5.1-V5-His (ThermoFisher Scientific) using compatible restriction sites ...
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bioRxiv - Microbiology 2020Quote: ... and 9.8 μL of highly deionized Hi-DiTM formamide (Applied Biosystems, France). Samples were analyzed by Eurofins genomics (Ebersberg ...
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bioRxiv - Biophysics 2021Quote: ... The His-MBP tag was removed by cleavage with TEV protease (Invitrogen). Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 8 were initially cloned into pCDNA3.1/myc-His A (Invitrogen) and were later subcloned into pBJ5 vector using the NEBuilder HiFi DNA assembly kit (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... and ACE2 His constructs were produced in Expi293 cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All anCas genes were cloned into pBAD/His expression vector (ThermoFisher Scientific) and transformed in E ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal mouse anti-6x-His antibodies (Epitope-tag-clone: HIS.H8, Thermo Fisher) were employed ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS; Gibco™), 1% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The His-tagged protein was purified with Nickel-NTA resin (Thermo Scientific) in 20 mM Sodium Phosphate (pH 7.0 ...
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bioRxiv - Neuroscience 2019Quote: ... GBA was cloned into pcDNA3.1/myc-His A vector (Thermo Fisher Scientific) after digestion with EcoRI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT2 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS, Life Technologies), 2 mM GlutaMax (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Microbiology 2021Quote: ... Dynabeads™ His-Tag isolation and pulldown were purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT4 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Biophysics 2022Quote: ... blots were incubated with 6x-His Tag Monoclonal Antibody (Thermo Fisher Scientific). As a control ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122). Cells were maintained in humidified incubators at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
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bioRxiv - Neuroscience 2022Quote: ... and desmin were inserted into expression vectors pcDNA6 V5-His A (Invitrogen) using BamHI/XhoI ...
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bioRxiv - Microbiology 2022Quote: ... which is RPMI-1640 media containing 10% heat-inactivated (HI)-FBS (Gibco) and 1x penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678) and 1X TBE Running Buffer ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher), and rabbit polyclonal anti-mCherry (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... His-VASP was bound to HisPur™ Ni-NTA Resin (ThermoFisher #88221), washed twice with 5 CV of wash buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Immunology 2023Quote: His-tagged proteins were purified with HisPurTM Ni-NTA resin (Thermo Fisher). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...