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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured and purified from the supernatant by Pierce High Capacity NeutrAvidin Agarose (Thermo Fisher Scientific). After washing six times with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated spike protein was purified using a 40K MWCO 2 mL Pierce Zeba™ desalting column (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A small amount of the purified proteins were biotinylated using the EZ-link BMCC-biotin system (ThermoFisher Scientific). ≈1 mg BMCC-biotin was dissolved directly in 100% DMSO to a concentration of 8 mM for labeling ...
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bioRxiv - Neuroscience 2020Quote: ... GAD65/67 and gephyrin proteins were detected using biotinylated goat anti-rabbit and streptavidine Alexa 488 (Molecular Probes; catalog No ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2) and surface proteins were biotinylated using 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 40 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: Purified RECQL5 and sfGFP proteins were biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Scientific #21435) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated (Thermo Scientific Cat# 29997) at 1:100 ratio ...
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bioRxiv - Immunology 2022Quote: ... and biotinylated CD15 primary (Invitrogen) with PECy7-conjugated Streptavidin (eBioscience) ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated capture antibodies (AT8: Invitrogen, REF MN1020B ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Immunology 2021Quote: ... all PE labelled and prefusion SARS-CoV-2 S-Avi tag conjugated with streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2-specific IgG+ memory B cells were sorted and seeded on MSC (mesenchymal stromal cells ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...
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bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
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bioRxiv - Immunology 2024Quote: ... Cells were transfected with C9-tagged SARS-CoV-2 S protein or with empty vector (pcDNA3.1) using Lipofectamine 2000 (Invitrogen) and maintained in antibiotic free DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Biochemistry 2019Quote: ... Each protein was first purified by metal affinity chromatography using 2ml of His purTM cobalt column (Thermo Scientific). The tag was removed by cleavage with TEV protease followed by purification on a second cobalt affinity chromatography ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Pathology 2022Quote: TRP120 and TRX recombinant proteins were desalted using Zeba spin desalting columns (Thermo Fisher Scientific, MA) as indicated by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 µL of the cleared lysates were added with 20 µL Recombinant Protein G Agarose (Invitrogen) and rotated for 30 min at 4 °C to preclear ...
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bioRxiv - Microbiology 2021Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quantification of the recombinant proteins was performed by Pierce™ BCA Assay (Thermo Fisher Scientific 23225) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Microbiology 2022Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 647 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The Pbc43opt recombinant protein was extracted from inclusion bodies using the Inclusion Body Solubilization Reagent (ThermoFisher). The solubilized protein was also purified using TALON® metal affinity resin ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Serum was used for antibody affinity purification to the STS1 recombinant protein using magnetic beads (Invitrogen). Validation of antibody was carried out by immunoblot with total protein extract from sts1 complemented lines and sts1 mutant as a negative control ...
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bioRxiv - Biochemistry 2022Quote: ... All recombinant proteins were examined by SDS-PAGE along with Pierce BSA mass standards (Thermo Fisher), and their concentrations were calculated from infrared scanning of Coomassie-stained gels (Odyssey Fc Imaging System ...