Labshake search
Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
-
bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
-
bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
-
“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
-
bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
-
bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
-
bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
-
bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
-
bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
-
bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
-
bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
-
bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
-
bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
-
bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
-
bioRxiv - Developmental Biology 2024Quote: ... The purified proteins were stored at −80°C and the protein concentration was determined using Bradford plus protein assay reagents (23238, Thermo Scientific).
-
bioRxiv - Microbiology 2022Quote: ... supernatants were mixed with either 0.8 μg/mL of rat anti HA antibody and 30 μL of pre-washed dynabeads protein G (Thermo Fisher; 10003D) or 40ul of pre-washed anti-Flag M2 affinity gel (Millipore Sigma ...
-
bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific), and extracts were loaded onto NuPAGE Bis–Tris SDS gels and immunoblots were visualized by LiCOR Odyssey system.
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the pHrodo-TDM1 fluorescent signal was normalized to the amount of protein at each time point.
-
bioRxiv - Cancer Biology 2021Quote: ... The total protein concentrations were determined using BCA protein assay kit (Thermo Scientific), and IFN-α and IFN-γ protein concentrations were measured using a human IFN-α ELISA kit (1110012 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific) and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... protein concentration of the supernatant was determined using BCA protein assay (Thermo Fisher) and 50 μg (1C and S2F ...
-
bioRxiv - Neuroscience 2020Quote: ... Total protein content was quantified using a BCA Protein Assay Kit (Thermo Scientific) and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad) ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific). Protein lysates were analyzed by SDS PAGE and Western blot using fluorescently-labeled secondary antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using a reversible protein stain (Memcode, Pierce, Thermo Fisher Scientific) and membranes were incubated with the following primary antibodies listed in Table S3 ...
-
bioRxiv - Cell Biology 2020Quote: ... The total protein content was measured using BCA Protein Assay Kit (ThermoFisher Scientific). The relative ALP activity was normalized to total protein content and expressed as units/g·protein.
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay kit (Thermo Fisher). An equal volume of 2x Laemmli buffer (BIO-RAD ...
-
bioRxiv - Cell Biology 2020Quote: ... and protein concentration was determined with the Pierce BCA protein assay kit (ThermoFisher). Insoluble materials were removed by centrifugation at 21,000 g for 30 mins at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Aliquots of lysates were solubilized in Laemmli sample buffer and equal amounts of proteins were separated on 12% SDS-PAGE gels ...
-
bioRxiv - Immunology 2021Quote: ... Protein concentration was quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific). Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sample protein concentrations were determined using the BCA Protein Assay Kit (Thermo Scientific). Primary antibodies were anti-Six1 (rabbit polyclonal HPA001893 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Extracted proteins were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and stored at −80°C until further analysis.
-
bioRxiv - Immunology 2020Quote: ... Protein concentrations were determined using the BCA protein assay kit (Thermo Scientific #A53225). Protein samples were separated by SDS–PAGE (Invitrogen #NW04125BOX) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using Pierce™ Coomassie (Bradford) Protein Assay Kit (ThermoFisher). Protein sample loading buffer (62.5 mM Tris-Cl pH6.8 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein was quantified using Pierce™ 660 nm Protein Assay Reagent (Thermo Scientific) and 1 mg (for adults ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher), and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Scientific, Rockford, IL).
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined with the BCA protein assay kit (Thermo Fisher 23227) using a bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2020Quote: ... AHA-conjugated proteins were biotinylated using the Protein Reaction Buffer kit (Thermo Fisher), following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... the protein bands were visualized by staining with Imperial Protein Stain (Thermo Scientific) for 1 hr followed by overnight de-staining with Milli-Q water ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein bands were visualized by staining with Imperial Protein Stain (Thermo Scientific) for 1 hr followed by overnight de-staining with Milli-Q water ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined with a Pierce BCA Protein Assay Kit (Thermo Scientific) and all samples were stored at -80°C until further use.
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were measured using bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Ten to 30 µg of protein samples were loaded on 10% sodium dodecyl sulfate polyacrylamide (SDS-PAGE ...
-
bioRxiv - Physiology 2021Quote: ... Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher) and mixed 1:1 with 2x Laemmli buffer (Bio-Rad ...