Labshake search
Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 7H Benzocyclohepten 7 one 2 amino 5 6 8 9 tetrahydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
-
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
-
bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
-
bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
-
Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
-
bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
-
bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
-
bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... we used 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) or FxCycle™ PI/RNase Staining Solution (Thermofisher ...
-
bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
-
bioRxiv - Neuroscience 2022Quote: ... 7-aminoactinomycin D (7-AAD, Thermo Fisher, A 1310) was added 1:50 as a cell death marker.
-
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
-
bioRxiv - Plant Biology 2019Quote: ... coli or One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher Scientific). Depending on the selectable marker ...
-
bioRxiv - Plant Biology 2022Quote: ... Vectors were transformed into Escherichia coli One Shot OmniMAX 2 T1 (ThermoFisher Cat# C854003). CRISPR-Cas9 expression vectors carrying gRNA_W or gRNA_F were transformed in Agrobacterium tumefaciens (GV3101) ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µM PAGE-purified primers and one unit of Platinum Taq DNA polymerase (Invitrogen). qPCR signal from a mock control lacking antibody was measured to determine the efficiency of recruitment ...
-
bioRxiv - Neuroscience 2023Quote: ... At 5-6 dpf each FoxP2.A:FingR(PSD95)+ larva was placed into individual wells of a 6-well plate (Thermo Fisher Scientific) containing approximately 10mL of fish water ...
-
bioRxiv - Microbiology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 µL bed volume (ThermoFisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from 30 flies of 5-7 days old by TRIzol reagent (Invitrogen). The genomic template was removed using DNase (Takara) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
-
bioRxiv - Biochemistry 2023Quote: ... 7 mM NaOH) followed by an incubation of 5 hrs with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
-
bioRxiv - Biochemistry 2023Quote: ... we incubated 5-7 μg of BACMID DNA with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 250 μL of Opti-MEM serum free media for 30 minutes at 23ºC ...
-
bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were grown at 37 °C under 5% of CO2 in DMEM Glutamax (Gibco) with 10% bovine fetal serum ...
-
bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc. ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 mM MgCl) with Zeba™ Spin Desalting Column with a 7 kDa MWCO (Thermo Scientific). The protein was used to prepare a serial dilution in a black 384-well ...
-
bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
-
bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 52h after plating 5 µM 5-ethynyl-2′-deoxyuridine (EdU, Life Technologies) was added for 20 h ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
-
bioRxiv - Biophysics 2021Quote: ... Cell nucleus were stained with DAPI (4’,6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
-
Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... All preparations were stained with 4’,6’-diamidino-2-phenylindole (DAPI, Invitrogen) for 10 minutes to label nuclei and mounted with Fluoroshield medium (Sigma) ...
-
bioRxiv - Bioengineering 2022Quote: ... Nuclei were counterstained with 4’,6’-diamidino-2-phenyliondole (DAPI; Invitrogen, D1306). Coverslips were mounted with ProLong™ Gold Antifade (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... 30 min incubation with 4′,6-diamidino-2-phenylindole (DAPI, Life Technologies) (1:1000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... slides were briefly stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) to reveal the nuclei ...
-
bioRxiv - Genomics 2020Quote: ... followed by nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen). Both bright field and nuclear staining images were separately taken using a Keyence BZ-X700 All-in-One microscope.
-
bioRxiv - Cancer Biology 2019Quote: ... ProLong Gold Antifade Mountant with 4′,6-diamidino-2-phenylindole (Life Technologies) was used for mounting ...
-
bioRxiv - Biophysics 2019Quote: ... DNA was stained with DAPI (4’,6-diamidino-2-phenylindole, Molecular Probes) at 2 µg/ml ...
-
bioRxiv - Microbiology 2019Quote: All samples were counterstained with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen, Fisher Scientific AG ...
-
bioRxiv - Biochemistry 2020Quote: ... USA) and 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Immunology 2020Quote: ... cells were incubated with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) for 10 minutes at room temperature in the dark ...
-
bioRxiv - Bioengineering 2021Quote: ... Nuclei were stained with 4’,6-Diamidin-2-phenylindol (DAPI, Invitrogen, #D1306) 1:10,000 in 0.01 M PBS.
-
bioRxiv - Neuroscience 2021Quote: ... at 1:200 and (4’,6-diamidino-2-phenylindole) DAPI (Fisher Scientific) at 1:10,000.
-
bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Thermo Fisher Scientific) was added to visualize cell nuclei ...
-
bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Thermo Fisher Scientific) was added to visualize cell nuclei ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were afterwards counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen). The primary antibodies used were nephrin (Progen ...
-
bioRxiv - Microbiology 2020Quote: ... Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) to calculate the CC50 values ...