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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Recombinant Human TNFRSF4 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... 2% FCS (ThermoFisher), and 50 μg/mL gentamicin (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (InVitrogen) and either 1 µg/mL puromycin (DIvA cells ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Gibco), 1% non-essential amino acid (NEAA ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco) in RPMI ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% FCS (Invitrogen), 5% human serum (HS) ...
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bioRxiv - Cell Biology 2024Quote: ... 44% FCS (Gibco), and 6% DMSO (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% FCS (Gibco), and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 10% FCS (Gibco), penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % FCS (Gibco), 1 % PenStrep (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (Gibco), 1X Pen-Strep (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS (Gibco), 25 mM HEPES (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured and purified from the supernatant by Pierce High Capacity NeutrAvidin Agarose (Thermo Fisher Scientific). After washing six times with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated spike protein was purified using a 40K MWCO 2 mL Pierce Zeba™ desalting column (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A small amount of the purified proteins were biotinylated using the EZ-link BMCC-biotin system (ThermoFisher Scientific). ≈1 mg BMCC-biotin was dissolved directly in 100% DMSO to a concentration of 8 mM for labeling ...
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bioRxiv - Neuroscience 2020Quote: ... GAD65/67 and gephyrin proteins were detected using biotinylated goat anti-rabbit and streptavidine Alexa 488 (Molecular Probes; catalog No ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2) and surface proteins were biotinylated using 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 40 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested and biotinylated peptides were captured (80 μl Pierce™ Streptavidin UltraLink™ Resin, ThermoFisher Scientific), resin was washed ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: Purified RECQL5 and sfGFP proteins were biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Scientific #21435) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed 6x 300μl PBST and developed with an anti-human Fc specific secondary antibody (Invitrogen, Carlsbad, CA) and TMB as described above ...