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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were sequenced and transfected along with lentiviral packaging plasmids into HEK293FT cells with Lipofectamine 3000 (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cloned plasmids were then isolated from their bacterial cultures using GeneJET Plasmid Miniprep Kit from Thermo Fisher scientific (catalog number-K0503).
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bioRxiv - Immunology 2021Quote: ... Plasmid of positive clones were extracted using the GeneJET Plasmid Miniprep Kit following the manufacture protocol (ThermoFisher scientific). and tThe nucleocapsid cloning was confirmed by digestion with using BamHI and NheI FastDigest enzymes (ThermoFisher Scientific) ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: ... Plasmids were extracted with Favorprep Plasmid Extraction Mini Kit and cleaved with FastDigest SacI restriction enzyme (Thermo Scientific) to eliminate the toxin plasmids ...
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and total DNA amounts of 5μg (2.5μg donor vector plus 1.25μg of each TALENS arm plasmid) or 2μg (1μg piggybac vector plus 1μg of transposase plasmid) were combined with Lipofectamine Stem (ThermoFisher, STEM00003) in Opti-MEM (ThermoFisher 11058021) ...
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bioRxiv - Neuroscience 2020Quote: ... Large quantities of purified plasmids were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific). The cloned sequences of ItypOrco ...
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bioRxiv - Immunology 2019Quote: ... plasmids were treated with DpnI to remove non-mutated parental plasmids and cloned into Stbl2 cells (Life Technologies) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 5 × 104 E14Tg2a cells were transfected with 100 ng of reporter plasmids (pGL4.10[luc2] and their derivatives) and 1 ng of pGL4.70[hRluc] control plasmid using Lipofectamine 2000 (Invitrogen) in 96-well plates ...
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bioRxiv - Biophysics 2019Quote: ... were selected for their suitability for transformation with a plasmid containing ampicillin resistance (pRSET/EmGFP plasmid; Invitrogen, UK).
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral plasmids and packaging plasmids were transfected into lentiX-293T cells using Lipofectamine 2000 (Invitrogen, Carlsbud, CA) and lentivirus-containing supernatants were harvested after 3 days ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were then extracted and purified using the PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific, Cat# K210006) for downstream experiments ...
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bioRxiv - Microbiology 2024Quote: Isolation of plasmid DNA was carried out with Genejet Plasmid Miniprep kit (Thermo Fisher Scientific, Pittsburgh, PA, USA), according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 750 ng of plasmid purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was isolated using the GeneJET Plasmid Miniprep Kit (Cat #K0503; Thermo Fisher Scientific, MA, The US). Isolated plasmids were prepared for transformation by linearizing with EcoRI restriction enzyme according to the supplier’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The confirmed plasmid was prepared in large quantities using the PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). The plasmid concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng firefly luciferase plasmid and 112.5 ng GFP or different eIF1 plasmids using Lipofectamine 2000 (Thermo Fisher). After 24 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 µL of plasmid-lipofectamine mixture containing 75 ng plasmid and 165 ng Lipofectamine 2000 (Invitrogen, 11668-019) in Opti-MEM (Gibco ...
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bioRxiv - Genomics 2023Quote: ... Colonies were selected and plasmid DNA was extracted using The PureLink™ Quick Plasmid Miniprep Kit (Thermofisher Scientific) and sent for Sanger sequencing to identify the integrity and quality of the inserted enhancer fragment ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transfections were performed using 2 μg plasmid per 3.5-cm well plates using Lipofectamine 2000 (Life Technologies) transfection reagent according to the manufacturer’s recommendations at 48 hours before harvest ...
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bioRxiv - Systems Biology 2023Quote: ... coli (High Efficiency) cells and the plasmid DNA was purified using the PureLink™ Plasmid Purification Kit (Invitrogen). Three oligonucleotide pools were cloned separately and pooled together to acquire the final 7mer library ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids containing the promoter regions were ordered via gene synthesis as Gateway-compatible donor plasmids (ThermoFisher Scientific). Promoter sequence and expression cassette from donor constructs pEntry.P4P1R.EF1a and pEntry.MiniCas9V2 (Maria et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pWS082-CAN1 sgRNA plasmid (Table S1) was extracted using the GeneJet Plasmid miniprep kit (ThermoFisher Scientific). Accuracy of the cloning was confirmed through DNA sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... The BUB1 plasmids or siRNA with plasmids were transfected with Lipofectamine 2000 (Catalog No. 11668500, Thermo Fisher Scientific) per manufacture’s protocol.
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Microbiology 2021Quote: ... which is composed of two ACE2 ectodomains linked to the Fc portion of the human IgG (Anand et al., 2020).Alexa Fluor-647-conjugated goat anti-human Abs (Invitrogen) were used as secondary antibodies to detect ACE2-Fc and plasma binding in flow cytometry experiments.
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Molecular Biology 2024Quote: ... Adult human dermal fibroblast (HDFa) cells (Cascade Biologics C-013-5C) were maintained in Human Fibroblast Expansion Basal Medium (Gibco) supplemented with Low Serum Growth Supplement (Gibco).
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bioRxiv - Bioengineering 2020Quote: ... using PureLink Quick Plasmid Miniprep Kit (Invitrogen) and were sequence verified ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500ng of T7 calmodulin plasmid (Invitrogen, #454002) was also used in the following cell free expression reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and Plus reagent (Invitrogen # 11514015) were mixed at 1:1 and incubated for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and PureLink HiPure Plasmid Midiprep Kit (Invitrogen). The DNA sequences were read by dye terminator cycle sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).