Labshake search
Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... were transfected with a plasmid solution containing 1 ml of Opti-MEMTM (Gibco), 60 µl of 1mg ml-1 polyethylenimine (PEI ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies and Alexa Fluor 594 Phalloidin (1:100; Molecular Probes, UK; A12381) were diluted to desired concentrations in blocking solution and allowed to incubate for 1 hour before three washes with 0.1% Triton X-100 in PBS (PBST) ...
-
bioRxiv - Cell Biology 2024Quote: Anti-goat IgG conjugated with Alexa Fluor 488 (Invitrogen, Thermo scientific, Waltham, MA, USA)
-
bioRxiv - Cell Biology 2024Quote: Anti-goat IgG conjugated with Alexa Fluor 488 (Invitrogen, Thermo scientific, Waltham, MA, USA)
-
bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with scrambled siRNA (Silencer Select negative control, Thermo Fisher 4390843 or Silencer Negative Control 1, Thermo Fisher AM4611) and APMAP siRNA (Silencer Select siRNA ...
-
bioRxiv - Cell Biology 2024Quote: RPMI 1640 medium (Gibco; Grand Island, NY, USA)
-
bioRxiv - Cell Biology 2024Quote: Coverslips (Thermo scientific, Waltham, MA, USA)
-
bioRxiv - Cell Biology 2024Quote: ... Tandem RNAi (10 nM)/plasmid transfection for rescue experiments were performed at 48 h mark using Lipofectamine 3000 (Invitrogen L3000015) as follows.
-
bioRxiv - Cell Biology 2024Quote: ... or Femto maximum sensitivity substrate (Thermo Fisher) for OXPHOS cocktail ...
-
bioRxiv - Cell Biology 2024Quote: Superfrost plus slides (Thermo scientific, Waltham, MA, USA)
-
bioRxiv - Cell Biology 2024Quote: ... 3) Oxidative stress induction using 70 μM tert-Butyl hydroperoxide (Thermo Fisher 180345000) for 16-18 h ...
-
bioRxiv - Cell Biology 2024Quote: ... fat bodies were mounted on slides in SlowFade Gold antifade reagent with DAPI (Invitrogen). Tissues were imaged by confocal fluorescence microscopy (40X oil ...
-
bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse AF594 (Thermo Fisher Scientific; A21203), donkey anti-rabbit AF488 (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Cell Biology 2024Quote: ... and streptomycin (100 µg/ml) (Life Technologies) in a humidified incubator at 37°C and 5% CO2.
-
bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 647 goat anti-rat IgG (Invitrogen, catalog A-21247) at 2 μg/mL and Alexa Fluor 647 goat anti-mouse F(ab’)2 (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... The secondary antibody used was Alexa-Fluor 488 goat anti-rabbit IgG (Molecular Probes). In addition ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% FBS (Gibco), and 100 units penicillin/100 μg/mL streptomycin (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated in digestion solution (3 mg/mL collagenase D [Roche] in DMEM/F-12 [Gibco]) for 45 min at 37 °C with intermittent vortex mixing to dislodge soft tissues ...
-
bioRxiv - Cell Biology 2024Quote: ... Kinase reactions were terminated by adding 4X LDS loading buffer (NuPAGE LDS, Invitrogen, Carlsbad, CA) and loaded on precast 4 to 12% bis-tris gels (Life Technologies/Thermo Fisher) ...
-
bioRxiv - Cell Biology 2024Quote: ... All primary antibody incubations were followed by incubation with secondary horseradish peroxidase (HRP)– conjugated antibody (Pierce, Thermo Fisher) in 5% milk and 0.1% Tween 20 in TBS and visualized using Super Signal West Pico or Femto Chemiluminescent Substrate (Pierce ...
-
bioRxiv - Cell Biology 2024Quote: ... and loaded on precast 4 to 12% bis-tris gels (Life Technologies/Thermo Fisher). The kinase substrates were visualized using a phospho-specific antibody against pSTAT1-S727.
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was performed with TaqMan fast universal PCR master mix (Applied Biosystems; Foster City, CA). RT reaction conditions ...
-
bioRxiv - Cell Biology 2024Quote: ... The PCR products were detected using an Applied Biosystems ViiA 7 Real-Time PCR System (ThermoFisher Scientific). Relative gene expression was determined by the 2-ΔΔCt method ...
-
bioRxiv - Cell Biology 2024Quote: RNA was extracted from 0.5 - 1 million cells with the RNeasy kit (Invitrogen). One-step q-RT-PCR was performed ...
-
bioRxiv - Cell Biology 2024Quote: ... the pellet was resuspended in DMEM/F-12 (Gibco) supplemented with 10% FBS (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysates were loaded on pre-cast 4 to 12% bis-tris protein gels (Life Technologies). Proteins were transferred to nitrocellulose membranes using the iBLOT system (Life Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... at 2 μg/mL and Alexa Fluor 647 goat anti-mouse F(ab’)2 (Invitrogen, catalog A-21237) at 2 μg/mL were used ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes using the iBLOT system (Life Technologies) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10 % foetal bovine serum (FBS, Gibco) and 100 units penicillin/100 μg/mL streptomycin (Sigma-Aldrich).
-
bioRxiv - Cell Biology 2024Quote: ... followed by the addition of protease and phosphatase inhibitors (Thermo Fisher). Purified active mTOR kinase domain(MiliporeSigma ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% bovine calf serum (BCS) (Thermo Fisher Scientific), 50 mM GlutaMAX (Life Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... The dsRNA was synthesized using MEGAscript™ RNAi kit (Invitrogen), following the manufacturer instructions and stored at −20 °C until its use ...
-
bioRxiv - Cell Biology 2024Quote: ... and leupeptin (1 µg/ml) (Cell Signaling Technologies, Thermo Fisher)] supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10 % FBS (Gibco), 1.2 g/L sodium bicarbonate (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... in 5% milk and 0.1% Tween 20 in TBS and visualized using Super Signal West Pico or Femto Chemiluminescent Substrate (Pierce, Thermo Fisher) on a ChemiDoc-MP imaging system (Bio-Rad).
-
bioRxiv - Cell Biology 2024Quote: ... Explants were maintained in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12, Gibco) supplemented with 10 % foetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2024Quote: Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific, A20006) was used to directly label integrin antibodies following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 mM GlutaMAX (Life Technologies), penicillin (100 U/ml) ...
-
bioRxiv - Cell Biology 2024Quote: ... and loaded on precast 4 to 12% bis-tris gels (Life Technologies/Thermo Fisher). The kinase substrates were visualized using a phospho-specific antibody against pSTAT1-S727.
-
bioRxiv - Cell Biology 2024Quote: ... Equal amounts of lysates were resolved under reducing conditions in 4%-20% Novex WedgeWell gels (Invitrogen) and transferred to PVDF membranes (Advansta ...
-
bioRxiv - Cell Biology 2024Quote: Thermocycling was performed on the QuantStudio 7 Flex Real-Time PCR machine (Applied Biosystems, UK) using TaqMan Universal PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1 mM sodium pyruvate (Gibco). T cell activation was done on anti-CD3 (5 μg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was carried out on a StepOne Real-Time PCR System (Applied Biosystems) according to the following programme ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated with 1-2 μM MitoSOX™ Mitochondrial Superoxide Indicator (Invitrogen) for 15 min at 37°C prior to harvesting ...
-
bioRxiv - Cell Biology 2024Quote: ... the yield was quantified using the Qubit fluorometer (Invitrogen) and the absence of genomic DNA contamination was checked using the TapeStation automated electrophoresis system (Agilent) ...
-
bioRxiv - Cell Biology 2024Quote: ... Myc-tag immunoprecipitation (IP) was carried out using the Myc-tag magnetic IP kit (Thermo Fisher Scientific, 88844), following the manufacturer’s recommended protocol.
-
bioRxiv - Cell Biology 2024Quote: ... and SYTOX™ Green Ready Flow™ Reagent (Invitrogen, #R37168, 1:10.000) before sorting into 384-well plates containing lysis buffer (described below ...
-
bioRxiv - Cell Biology 2024Quote: ... After sorting cells were resuspended in organoid medium (ENR) (DMEM/F-12, #11320033, Gibco; 1X PenStrep, #15140122, Gibco; 1X B27 supplement, #17504044, Gibco; 1X GlutaMAXTM supplement ...
-
bioRxiv - Cell Biology 2024Quote: ... The combined cell mixtures were resuspended in minimal organoid medium (DMEM/F-12, Gibco, #11320074; 1X PenStrep, Gibco, #15140122; 1X B27 supplement, Gibco, #17504001 ...