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Citations for Thermo Fisher :
8651 - 8700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Samples were analyzed on a Dionex UHPLC system coupled with a hybrid quadrupole–high-resolution mass spectrometer (Q Exactive Orbitrap; Thermo Scientific), using an Acquity UPLC BEH C18 column (Waters) ...
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bioRxiv - Microbiology 2024Quote: ... either a goat anti-rabbit IgG-Alexa 488 (Invitrogen, # A-11008), a donkey anti-mouse IgG-Alexa 488 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... After annealing and DNase treatment using the TURBO DNA-free Kit (Life Technologies), the purified dsRNAs were resuspended in nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... all cDNA reactions were subjected to a specific PCR reaction for CHIKV genome amplification with the Phusion PCR kit (Phusion High-Fidelity DNA polymerase kit, Thermo Scientific, ref: F530L). 5 pairs of primers were used to span all CHIKV genome (Table 3 ...
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bioRxiv - Microbiology 2024Quote: ... a total of 0.5 μg of RNA per well was transfected with Lipofectamine MessengerMAX Transfection Reagent (Invitrogen, # LMRNA001) at a respective ratio of 1:2 (RNA:Lipofectamine) ...
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bioRxiv - Microbiology 2024Quote: ... Signal was visualized using the SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific, 34580). Images were taken using the Bio-Rad ChemiDoc MP Imaging System and images were processed using Bio-Rad Image Lab software.
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bioRxiv - Cell Biology 2024Quote: ... 1x N2 (17502048, Gibco), 50ng/mL mEGF (PMG8043 ...
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bioRxiv - Microbiology 2024Quote: ... or an anti-rabbit IgG-HRP antibody (Invitrogen, ref: 31460) was used ...
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bioRxiv - Microbiology 2024Quote: ... Isolated RNA was then subjected to DNase treatment using the TURBO DNA-free Kit (Life Technologies) to assure removal of any residual genomic DNA from the RNA samples.
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bioRxiv - Microbiology 2024Quote: ... 1 ug of RNA isolated from JE2 WT was subjected to reverse transcription by using 5՚-phosphorylated primer pepV_TSS_R1 and the first strand cDNA synthesis kit (Invitrogen, Superscript III First-Strand Synthesis System) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were centrifuged in MicroAmp™ Optical 96-Well Reaction Plate (Applied Biosystems) at 2325 g for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: RNA was harvested using TRIzol Reagent (Invitrogen), ensuring RNase-free conditions ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips were mounted using Prolong gold antifade (Molecular Probes, Life Technology) and sealed with fixative ...
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bioRxiv - Microbiology 2024Quote: ... were acquired from Thermo Fisher. EGFP and ICP27 primers for SYBR Green and fluorescent applications were purchased from IDT.
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bioRxiv - Microbiology 2024Quote: ... followed by actin and DNA staining using rhodamine phalloidin (Invitrogen) and DAPI (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... in a QuantiFast light cycler (Applied Biosystems). The relative transcript levels were estimated by using the comparative threshold cycle method (ΔΔCT ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...
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bioRxiv - Microbiology 2024Quote: ... and quantified using the Qubit RNA Broad-range Assay with the Qubit Fluorometer v3.0 (Thermofisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Excised liver was stored in RNAlater™ Stabilization solution (Thermofisher Scientific, MA, USA), at room temperature while in the field and then at 4°C for longer term storage ...
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bioRxiv - Microbiology 2024Quote: ... The SyPro orange dye was added to a final concentration of 1 X Protein Thermal Shift™ Dye (Thermofisher) in the reaction mixture ...
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bioRxiv - Microbiology 2024Quote: The DNA extractions were performed with the MagMax Microbiome kit from ThermoFisher following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... RAW 264.7 cells were seeded at a density of 5 × 105 cells/well in 24-well tissue culture plates (ThermoFisher) for bacterial uptake assay and in 12-well plates with round coverslips for immunofluorescent microscopic analysis ...
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bioRxiv - Microbiology 2024Quote: ... by incubating 20 μl of the sample in 180 μl dsDNA 1x solution (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2024Quote: ... The digested PCR products and backbone plasmid were ligated using T4 DNA ligase (Thermo Fisher Scientific). The mixture was transformed into CaCl2-competent E ...
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bioRxiv - Microbiology 2024Quote: ... equal quantities of RNA (1 μg) were either reverse transcribed to cDNA using the TaqMan kit (Applied Biosystems) or directly used with the ONE STEP TAQ QPCR kit (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... which were securely sealed with a sterile membrane (Thermo Scientific, USA) to prevent evaporation ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT (human immortalized keratinocytes) and HeLa OSLO cells were cultured in DMEM medium (ThermoFisher) supplemented with 10% FBS and 2 mM glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120 kV ...
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bioRxiv - Cell Biology 2024Quote: ... and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120 kV ...
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bioRxiv - Cell Biology 2024Quote: RNAi was performed with Lipofectamine RNAimax (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA) assay following the manufacturer’s protocol and then stored at −80°C until use (Pierce protein assay kit; ThermoFisher Scientific, USA). The proteins were visualized by running a 4-20% SDS-PAGE (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: Cells were stained with 200 µL of serum free media containing CellTracker™ Orange (Invitrogen; Table S4) dye at specified concentrations for 30 min followed by replacement with 1 mL PBS ...
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bioRxiv - Microbiology 2024Quote: ... dispar PSMC1 and SHOC2 constructs were generated by RT-PCR amplification from total RNA isolated from LD652 cells using Superscript III reverse transcriptase (Thermo Fisher), iProof DNA polymerase ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed with TaqMan Gene Expression Assays (Thermo Fisher Scientific) for FOS ...
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bioRxiv - Microbiology 2024Quote: ... De Man– Rogosa–Sharpe (MRS) media was obtained from Gibco and mixed as either broth or agar according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... cDNA synthesis was carried out with SuperScript First-Strand Synthesis System kit (Thermo Fisher Scientific). qPCR was performed with TaqMan Gene Expression Assays (Thermo Fisher Scientific ...
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Intratumoral Microbiome of Adenoid Cystic Carcinomas and Comparison with other Head and Neck CancersbioRxiv - Microbiology 2024Quote: ... and Qubit Fluorometer (Thermo Fisher Scientific). The amplicons were pooled in equimolar ratios ...
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bioRxiv - Microbiology 2024Quote: Phage suspension of interest with a concentration of >1010 PFU/ml was pretreated with 20 U DNase I (ThermoFisher Scientific, MA, USA) and 2 µL RNase A/T1 Mix (4 µg RNase A ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto a Biodyne B Nylon Membrane (Thermofisher Scientific) at 30V for 2 hours on ice in 0.5% TBE using the Novex XCell II Blotting System (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were linearized at a NotI site positioned in the polylinker downstream from the I-PpoI ORF and transcribed using MEGAscript T7 kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 1% antibiotic/antimycotic (Gibco). LD652 cells were cultured as described previously in a 1:1 mixture of Grace’s Insect Media (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... with 10% (v/v) decomplemented fetal bovine serum (dFBS) (Gibco, Paisley, UK) and 1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... with Oligo (dT)18 primers (Thermo Fisher Scientific) by following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 1% Penicillin-Streptomycin (Gibco, 15140122), 10% FBS (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated across a 1mm × 5cm C18 column (1.9 ml Hypersil Gold, ThermoFisher) with a linear gradient of 4% - 40% CH3CN and 0.3% formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... Each cDNA sample was amplified in triplicate using the SYBR Green and Platinum Taq polymerase (Thermo Fisher Scientific, S7567) on a 7900HT Real-Time PCR instrument (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: The grid was imaged on a Talos Arctica (Thermo Fisher) equipped with a 200 kV field emission gun and K2 camera ...
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bioRxiv - Molecular Biology 2024Quote: ... Rat TM RNA was converted into cDNA using Superscript III reverse transcriptase (Thermo Fisher Scientific). cDNA was pre-amplified with pooled primers for the target genes ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA concentration and quality were determined using NanoDrop Spectrophotometer (Thermo Scientific, DE). RNA integrity (RNA Integrity Number ...
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bioRxiv - Molecular Biology 2024Quote: ... the culture medium was switched to osteogenic differentiation medium (α MEM (Thermo Fisher Scientific, A1049001) supplemented with 10% FBS ...