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Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... J774 cells were plated in 24-well plates (Thermo Scientific) at 2.5·105 cells/well density in DMEM supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Phusion Hot Start II High-Fidelity DNA polymerase (Thermo Scientific) was used for all PCR steps ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in tissue culture treated plates containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC # 30-2002) containing 10% fetal bovine serum (Gibco) until 60% confluent after which cells were transfected using Lipofectamine 2000 (Invitrogen # 11668019 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation pull-downs were performed using 1 mg of whole cell extracts with anti-HA antibody (Invitrogen # 26183). Protein G agarose beads (Millipore # 16-201 ...
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bioRxiv - Cell Biology 2024Quote: ... until 60% confluent after which cells were transfected using Lipofectamine 2000 (Invitrogen # 11668019) with mammalian expression plasmids expressing pRK5-HA-TC-hGAPDH ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of RNA was quantified using the Qubit™ dsDNA HS Assay Kit (Q32851) and Qubit™ RNA HS Assay Kit (Q32855) (Thermo Fisher Scientific, Waltham, USA).
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bioRxiv - Microbiology 2024Quote: The amplified DNA was quantified using gel electrophoresis and Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). Ultrasonic random shearing (Covaris M220 ...
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bioRxiv - Microbiology 2024Quote: ... together with pCMV.Dr8.2 and pCMV.VSV.G in a ratio of 10:10:3.5 using Lipofectamine 2000 (ThermoFisher Scientific). Lentiviral supernatant was harvested 48 h later and then filtered to be concentrated through a Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2024Quote: ... while in case of home collection the sample was stored in 2 ml cryovials with 600 μl RNALater (Ambion/Invitrogen, Carlsbad, CA), and later stored at –80°C upon arrival to the lab at the University of Minnesota.
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bioRxiv - Microbiology 2024Quote: ... Concentration was determined with Nanodrop (ThermoFisher Scientific) and Qubit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction and cDNA synthesis were performed using QIAgen kits per the manufacturer’s directions and performed qPCR on a Quantstudio RealTime PCR machine (ThermoFisher) using SYBR Green ...
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bioRxiv - Microbiology 2024Quote: Extracted ion chromatograms of the [M-H]- (dA, dT, dC, 5hmdC, 5fdC, 5cadC)/[M+H]+ (5ghmdC) forms were integrated using Tracefinder software (ThermoFisher Scientific, MA, USA). Relative abundance in each sample was calculated by normalizing the peak area of each peak by the peak area of the dG signal in each specific sample ...
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bioRxiv - Microbiology 2024Quote: ... Transient siRNA knockdown was achieved by forward transfection of LD652 with Cellfectin II (Gibco; Table S4) according to the manufacturer’s protocol for 48 h prior to infection ...
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bioRxiv - Microbiology 2024Quote: ... Additional bacterial effector genes were added to the pENTR library by Gateway® Cloning using BP Clonase II (Invitrogen; Table S4), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and Qubit (Invitrogen) using the broad range and/or high-sensitivity assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Reverse transcription was performed on 4 μL of total RNAs using SuperScript IV VILO Master Mix (Invitrogen). qPCR was performed using 2 μL of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... in presence of penicillin/streptomycin (Gibco) at 37°C with 5% CO2.
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bioRxiv - Evolutionary Biology 2024Quote: ... and TurboDNAse (Ambion DNAfree kit) on the total amount of eluate to eliminate residual DNA ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µL RNase A/T1 Mix (4 µg RNase A, 10 U RNase T1, ThermoFisher Scientific, MA, USA) at 37°C for 30 min to remove host-originating nucleic acids ...
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bioRxiv - Microbiology 2024Quote: ... at StepOne Plus Real-Time PCR system (Applied Biosystems). The changes in transcript abundance were analyzed using the 2−ΔΔCt method and normalized to GAPDH levels ...
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bioRxiv - Microbiology 2024Quote: ... Cells were imaged using a 2X objective on an EVOS-FL fluorescence microscope (Thermo Fisher; Table S4) using RFP and GFP cubes ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... Translated proteins labeled with [35S] Met were resolved in a 4%-12% SDS-PAGE gel (Invitrogen), dried onto a Whatman filter paper ...
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bioRxiv - Microbiology 2024Quote: ... using LR Clonase II (Invitrogen; Table S4), following manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... while in case of home collection the sample was stored in 2 ml cryovials with 600 μl RNALater (Ambion/Invitrogen, Carlsbad, CA), and later stored at –80°C upon arrival to the lab at the University of Minnesota.
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and actin (mouse monoclonal, Invitrogen, # ACTN05 C4). Three washes for 5 mins with 1X TBS-Tween-20 were performed and the membranes were finally incubated with the corresponding secondary antibody (1:10000 dilution) ...
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bioRxiv - Microbiology 2024Quote: ... cells were labeled with DAPI (Invitrogen, # D3571) and coverslips mounted in ProLong Diamond Antifade (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... anti-rabbit Alexa fluor 594 (Invitrogen, A11012). Following 3 washing steps as above ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with DAPI (Invitrogen), and samples were mounted by using a gold antifade medium (P36961 ...
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bioRxiv - Cell Biology 2024Quote: ... and samples were mounted by using a gold antifade medium (P36961, ThermoFisher Scientific, USA). Slides were then analyzed by epifluorescent microscopy (E800 ...
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bioRxiv - Cell Biology 2024Quote: ... according to the manufacturer’s instructions and quantified by Nanodrop One C (Thermo Fisher Scientific, Madison, USA). 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio ...
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bioRxiv - Cell Biology 2024Quote: ... in Hank’s balanced salt solution (HBSS, Invitrogen) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Andres Merits) and anti-Phalloidin-A488 (F-actin, ActinGreen 488 Ready Probes reagent, Invitrogen, # R37110). Coupled antibodies were always incubated after the non-coupled ones ...
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bioRxiv - Microbiology 2024Quote: ... 400 ng of total RNA was reverse-transcribed using the SuperScript III Reverse Transcriptase kit (Life Technologies) and oligo(dT)15 primer (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... in Dulbecco Modified Eagle Medium (DMEM, Gibco, 41966-029) supplemented with foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... as above or in DMEM without Glc (Gibco, 11966-025) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... gDNA was quantified using a QBIT fluorometer (ThermoFisher) and subsequently sent for sequencing to Genewiz/Azenta company ...
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bioRxiv - Microbiology 2024Quote: ... 5 min on a SimpliAmp Thermal Cycler (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... 4.0 µl 5x VILO reaction mixture (ThermoFisher #11754050) were added ...
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bioRxiv - Cell Biology 2024Quote: ... each well containing 1x Taqman Fast-Advanced Master Mix (ThermoFisher #4444557), 1x Taqman Probe Kit (Supp ...
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bioRxiv - Cell Biology 2024Quote: C2C12 myoblasts were transiently transfected using Lipofectamine 3000 Transfection Reagent (Invitrogen, 100022052), with Deaf1-promoter-pGL4.20 ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs were extracted from cultured cells or tissues using TRIzol reagent (Invitrogen, 15596-018) according to manufacturer’s protocol and were reverse transcribed to cDNA with PureNA First Strand cDNA Synthesis Kit (Research Instruments ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was immediately purified using PureLink RNA Mini-Kits (ThermoFisher #12183018A), including a genomic DNA removal step (PureLink DNase kit for on-col-umn protocol ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with foetal bovine serum (FBS, Gibco, 10270-106, 5% v/v), L-glutamine (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... quantified using a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific), and examined by agarose gel electrophoresis to ensure their integrity ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of Glycoblue co-precipitant (Invitrogen, # AM9515). RNA was precipitated overnight at −20°C ...
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bioRxiv - Microbiology 2024Quote: ... a donkey anti-mouse IgG-Alexa 488 (Invitrogen, # A-31570), a goat anti-rabbit Alexa 647 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... either a goat anti-mouse-HRP antibody (Invitrogen, ref: 31430) or an anti-rabbit IgG-HRP antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... the upper aqueous phase was transferred to a new tube containing 500 μL of molecular grade isopropanol (Absolute isopropanol molecular grade, Fisher Scientific, # 67-63-0) and 1 μL of Glycoblue co-precipitant (Invitrogen ...