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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Media was replaced with mock or 2.5 µM DFT supplemented FluoroBrite (Thermo Fisher Scientific) at 24 hpi ...
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bioRxiv - Microbiology 2024Quote: ... medium was removed and samples were clicked with AO-6MT in FluoroBrite (Thermo Fisher Scientific) containing Hoechst 33342 as indicated ...
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bioRxiv - Microbiology 2024Quote: ... DNA was quantified using a NanoDrop spectrophotometer (Thermo Scientific) and diluted to 5 ng/µL ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with non-essential amino acids (Thermo Fisher Scientific) and 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2024Quote: ... and stained with secondary antibody goat anti-mouse AlexaFuor594 (Thermo Fisher Scientific), unless specified ...
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bioRxiv - Microbiology 2024Quote: ... BMDM were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) GlutaMAX (Gibco #10569-010) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Microbiology 2024Quote: ... Bar-coded libraries were prepared using the Ion Plus fragment library kit (Life Technologies) and the Ion Xpress DNA bar coding kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with secondary antibody (goat anti-rabbit AlexaFluor488, Thermo Fisher Scientific) diluted in blocking buffer containing 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2024Quote: Membrane potential was measured using the potentiometric fluorescent probe DiSC3(5) (Invitrogen). Mid-log phase cells were diluted to an OD600 of 0.4 ...
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bioRxiv - Microbiology 2024Quote: ... and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX). Fragments from ∼190-250bp in length were excised ...
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bioRxiv - Microbiology 2024Quote: ... The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher). Images were captured using an inverted fluorescence microscope (Olympus-IX73).
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bioRxiv - Microbiology 2024Quote: ... The fluorochrome-labeled antibodies used were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... AlexaFluor 594 anti-human IgG (Thermo Fisher). The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... coli strain BL21(DE3) pLysS (Invitrogen) using lysogeny broth (LB ...
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bioRxiv - Microbiology 2024Quote: ... and 1% Tryptose phosphate browth (Trp; Life Technologies).
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Amplified fragments were analyzed by capillary electrophoresis using a 3730 XL DNA Analyzer (Applied Biosystems). Chromatograms were analyzed using the Osiris software (ncbi.nlm.nih.gov/osiris) ...
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bioRxiv - Microbiology 2024Quote: ... followed by sequencing using the S5 Ion torrent technology (Thermo Fisher), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 100ng of equimolar mixture of the produced fragments were transfected using the Lipofectamine 3000 kit (Invitrogen) following the supplier’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The extracted DNA was electrophoresed on a 1% agarose gel and checked with a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, USA). PCR reactions were performed as described in a previous paper (Shen et al. ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were built adding to fragmented DNA barcode for sample identification and primers with Ion Plus Fragment Library Kit using AB Library Builder System (Thermo Fisher). To pool equimolarly the barcoded samples ...
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bioRxiv - Microbiology 2024Quote: ... Next steps included an emulsion PCR of the pools and loading on 520 chips performed using the automated Ion Chef instrument (Thermo Fisher), followed by sequencing using the S5 Ion torrent technology (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (Invitrogen, ref A10262, dilution 1:200), anti-OCT4 (StemAB ...
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bioRxiv - Cell Biology 2024Quote: ... and Ham’s F10 (RDH medium; Thermo Fisher Scientific) at 38°C in 5% CO2 until cell microinjection ...
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bioRxiv - Cell Biology 2024Quote: ... 106 B19_GFP_DPPA2mKO2 cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of pEOStagBFPplasmid plus 2 μg of the PBase-expressing vector pCAGPBase (Wang et al ...
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bioRxiv - Cell Biology 2024Quote: ... 106 cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of PB plasmid plus 2 μg of the PBase-expressing vector pCAGPBase (Wang et al ...
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bioRxiv - Cell Biology 2024Quote: ... 106 B19_GFP cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of pCAG-Cas9D10A-sgDPPA2(x2 ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-mouse (Alexa Fluor 647, Invitrogen, ref A21448), donkey anti-rabbit (Alexa Fluor 555 ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA yield was measured using a Qubit fluorometer (Invitrogen), RNA quality was assessed using a Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... The organic phase was then removed by dialysis (24 h, room temperature) using a 100 kDa MW cut-off cellulose ester membrane (Thermo Fisher Scientific, France). Empty nanoformulation was prepared according to the same protocol but in absence of drug in the organic phase ...
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bioRxiv - Microbiology 2024Quote: Baby Hamster Kidney 21 cells (BHK21; ATCC-CCL10) were grown at 37 °C with 5% CO2 in minimal essential medium (Life Technologies) supplemented with 5% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Microbiology 2024Quote: ... The reacting mixture flowed through a sample loop of pre-defined length and then was quenched by a four-fold molar excess of ascorbic acid (A61-100, Fisher Scientific) relative to the initial free chlorine ...
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bioRxiv - Microbiology 2024Quote: ... followed by incubation with a secondary α-rabbit antibody conjugated to Alexa Fluor 488 or Alexa Fluor 555 (Invitrogen) diluted 1:1,000 in PBS for 30 min ...
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bioRxiv - Microbiology 2024Quote: After Qubit quantification using Qubit® dsDNA HS Assay Kit and Qubit 2.0 fluorometer (Thermo Fisher) amplicons were sonicated (Bioruptor® ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with the gene-encoding plasmid or an empty vector control using Lipofectamine 2000 or Lipofectamine 3000 (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA was synthesized by Verso reverse transcription (Thermo Fisher Scientic) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... goat α-chicken IgY-Alexa647 (Thermo Fisher, cat# A-21449) (1:500 dilution ...
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bioRxiv - Microbiology 2024Quote: ... and T4 Polynucleotide Kinase (EK0031, Thermo Fisher Scientific, Waltham, MA). Fragments in the range of 300-800 bp were collected after electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products and the vector were mixed at a 1:3 molar ratio and ligated using T4 DNA ligase (Thermo Scientific). For HiFi cloning ...
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bioRxiv - Microbiology 2024Quote: ... doxycycline and NuPAGE™ LDS Sample Buffer (4×) from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA was prepared with the High Capacity cDNA reverse transcriptase kit (Thermo Fisher, cat# 4368814). Real-time qPCR amplification of the cDNA was completed with PowerUp SYBR Green master mix (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips were mounted with ProLong glass anti-fade mountant (ThermoFisher, cat# P36984) onto slides ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM EDTA) supplemented with protease inhibitors cocktail (ThermoFisher scientific). Cell lysates were then centrifuged to pellet cell debris and cleared supernatants were transferred to new tubes ...
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bioRxiv - Microbiology 2024Quote: ... Quantity and quality of genomic DNA were measured by Qubit High Sensitivity dsDNA Assay (Thermo Fisher Scientific) and Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... and Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific). Genomic libraries were prepared using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... RNA was reverse-transcribed into cDNA using Oligo dT and SuperScript IV Reverse Transcriptase (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The total protein content transferred on the nitrocellulose membrane for each sample was detected after incubation with Ponceau S staining solution (Thermo Scientific, cat# A40000279) prior to immunoblotting.
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bioRxiv - Microbiology 2024Quote: ... Real-time qPCR amplification of the cDNA was completed with PowerUp SYBR Green master mix (Thermo Fisher, cat# A25741), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was isolated with the Easy-DNATM kit (Invitrogen), following the manufacturer’s instructions ...