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Citations for Thermo Fisher :
801 - 850 of 7001 citations for ST2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were transfected with pX330 containing the target site using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Knockout clones were obtained by limiting dilution and KO confirmed by sequencing the target sites in the genome DNA and by flow cytometric analysis of GPI-APs ...
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bioRxiv - Immunology 2020Quote: ... HEK293 T cells were transfected with lipofectamine 3000 following the manufacturer’s procedure (Invitrogen, Carlsbad, CA, USA). Prefusion F (BV2129 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were transfected with the indicated expression vectors using Lipofectamine 3000 (Life Technologies L3000-015). 48 h after transfection ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the control and ADARB1 encoding pcDNA3 constructs using Lipofectamine 3000 (Invitrogen) and analyzed 72hrs post-transfection.
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral supernatants were generated by transient transfection of HEK293 cells according to Lipofectamine ™ 2000 (Invitrogen) transfection protocols and harvested 48 h after transfection ...
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bioRxiv - Genomics 2022Quote: HUVECs and pB-circRNA transfected HEK293 were grown in media supplemented with exosome-depleted (Life Technologies). HEK293 were induced with 3 μg/ml doxycycline (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... BAC DNA was transfected into HEK293/ACE2/TMPRSS2 cells using lipofectamine 3000 (Thermo Fisher, cat. L3000001). Two days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: Culturing of HEK293 (RRID:CVCL_0045) and HeLa cells (RRID:CVCL_0030) and their transfection using TurboFect (Thermo Fisher Scientific) was essentially done as described (Kessels et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific, Cat. #11995040). pCDH-EF1a-mCherry-EGFP-LC3B (RRID ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Gibco, cat 11965-092) + 10% FBS ...
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bioRxiv - Biophysics 2023Quote: 15 mL of HEK293 cells were plated at 0.625 × 106 cells / mL in DMEM (Gibco 11995065) supplemented with 10% FBS in a T75 flask ...
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bioRxiv - Biophysics 2023Quote: 15 mL of HEK293 cells were plated at 0.625 × 106 cells / mL in DMEM (Gibco 11995065) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa RIEP (Jurkin et al. 2014) and HEK293 Flip-In™ T-REx™ (FITR, Invitrogen) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM high glucose ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Hek293 Flp-In T-REx cells were additionally supplemented with 100 µg/ml hygromycin B (Invitrogen) and 10 µg/ml blasticidine (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected at 70 to 80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015) and incubated at 37°C for 2 days ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC;CRL-1573) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965-092) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... HEK293 cells were transfected with PX459 and pCR-Blunt II TOPO plasmids by Lipofectamine 3000 (Invitrogen) for 72 hr ...
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bioRxiv - Physiology 2024Quote: ... These cells were generated by transfecting HEK293 cells using Lipofectamine 2000 (Invitrogen; 2 μl per well) with D1ER cDNA (0.5 μg/well ...
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bioRxiv - Neuroscience 2023Quote: HEK293S GnTIˉ cells (ATCC CRL-3022) were cultured in FreeStyle™ 293 Expression Medium (Gibco, 12338018) containing 1% fetal bovine serum (FBS) ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... HEK293 cells were maintained at 37°C with 5% CO2 in DMEM media (ThermoFisher Cat #11965118) supplemented with 10% serum (Serum Plus II ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321 ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 and Lenti-X 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 and Lenti-X 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... both plasmids containing sgRNAs were transfected into naïve HEK293 cells by Lipofectamine 3000 (Thermo Fisher Scientific) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting constructs were transfected into HEK293 cells using the Lipofectamine 3000 Reagent (Invitrogen, Cat#L3000001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and Western blotting was performed with anti-His (ThermoFisher MA1-135), anti-HA (Roche 11583816001) ...
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bioRxiv - Developmental Biology 2020Quote: ... The Ion Hi-Q Sequencing 200 Kit (Thermo Fisher Scientific, UK) and the Ion Proton platform were used for analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Cell Biology 2021Quote: ... pRSET-His-GFP-3C-Survivin (pRSET vector from Thermo Fisher Scientific), pMCNcs-INCENP1-58 and pETM-His-TEV-Borealin10-280 or pETM-His-TEV-Borealin10-221 (pETM vector ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was bound to His-Pur Cobalt resin (Thermo Scientific) for 2 hours with rocking at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The Ion PGMTM Hi-QTM View OT2 kit (Life Technologies, USA) and Ion OneTouch DL instrument (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
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bioRxiv - Zoology 2019Quote: ... Blots were probed with rabbit anti-His antibody (Thermo Fisher Scientific) at 1 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... and His-BMAL1 FL were co-infected into monolayer HighFive (Invitrogen) insect cells for protein expression ...
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
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bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...