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Citations for Thermo Fisher :
8351 - 8400 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Abcam, USA) (Anti-E-cad/N-cad/Vimentin; 1:200, Cell Signalling, USA) (Rhodamine phalloidin dye, 6.6 µM, ThermoFisher Scientific, USA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... MEFs were transfected with the isolated N-TAP progranulin plasmid (courtesy of the UF CTRND) using Lipofectamine LTX Reagent with PLUS Reagent (Invitrogen 15338030) according to its included protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed 3 times for 5 min with PBS and mounted with imaging buffer at pH 7.4 (700 mM N-Acetyl-Cysteine (NAC; #160280250, ThermoFisher Scientific, USA) in ddH2O + 20% HEPES solution (#H0887 ...
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bioRxiv - Cancer Biology 2023Quote: Small RNA (<200 bp) was isolated from fibroids and matched myometrium (n= 5) using mirVana miRNA isolation kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Immunoreactivity was detected using the ECL Western blot detection reagent LumiGLO (KPL) and exposed to Super RX-N films (Fujifilm) or revealed with iBright™ Imaging System (ThermoFisher). Protein expression was normalized to α-actinin or Gadph ...
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bioRxiv - Developmental Biology 2023Quote: ... and connective tissue were scraped away from the biopsy before digesting O/N at 4°C in HBSS without Ca2+ and Mg2+ (Thermo Fisher) containing dispase at a final concentration of 500 caseinolytic units/mL (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminally truncated PPR56 coding sequences were amplified with classic PCR approach using Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific) as described [23] to retain 14 native amino acids upstream of the most N-terminal completely retained PPR (Fig ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... All the IHO1 truncations were expressed with the 3C HRV cleavable N-terminal MBP tag in suspension culture of High Five™ Cells (Invitrogen) in Sf-900™ III SFM medium ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... The PSA-NCAM+ cells were re-plated at a density of 50 × 103 cells per cm2 to the plate coated with vitronectin (VTN-N, 5 μg/mL; ThermoFisher Scientific) and laminin (rhLaminin-521 ...
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bioRxiv - Neuroscience 2023Quote: The forebrain sections were incubated with Blue NeuroTrace Fluorescent Nissl Stains (1:100 dilution by PBS, N-21479, Thermo Fisher Scientific) at room temperature for 2-3 hours ...
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bioRxiv - Plant Biology 2023Quote: ... OLV-FL or one of the truncated OLV versions were shuttled simultaneously with PYE into the destination vector pBiFC-2in1-NN (Grefen and Blatt, 2012) (N-terminal nYFP and cYFP fusions) via multisite LR reaction (Gateway, Thermo Fisher). Hereby pBiFC-2in1-NN:OLV-FL-PYE and pBiFC-2in1-NN:PYE-OLV-FL (additionally all truncated OLV versions were cloned into pBiFC-2in1-NN combined with PYE as described for OLV-FL ...
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bioRxiv - Plant Biology 2023Quote: ... For N-terminal fusions the pDONR entry clones with PYE and OLV sequences were used for Gateway LR reaction (Gateway, Thermofisher, Scientific) to generate the destination vectors based on pH7WGY2 (N-terminal YFP ...
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bioRxiv - Biochemistry 2023Quote: ... Dried lysed cellular lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Physiology 2023Quote: ... the tissue was stained with NeuroTrace™ green fluorescent Nissl stain (1:200 in PBS, Life Technologies, catalog No. N-21480) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then reverse transcribed using random primers with a N-terminal amine group and Revert-aid H-minus reverse transcriptase (Thermo Fisher). On the second day ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Cell Biology 2023Quote: From Expi293 cells: Constructs in pCMV10 vector with N terminal 3xFLAG tag were transfected into Expi293F cells (A14527; Thermo Fisher Scientific) with PEI (Polyscience ...
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bioRxiv - Immunology 2023Quote: COS-7 cells were cultured for 12 h on glass coverslips in a 24-well plate and then co-transfected with pcDNA3.1-NOX2-N-Clover and EROS-C-mRuby2 using Lipofectamine 3000 (Thermo Fisher Scientific) for 24 h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... and 474/238), or fetal bovine serum (FBS, n = 542/415, 384/184, and 459/308) using a live/dead kit (Thermofisher, #L3224). Cell suspensions were centrifuged ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Microbiology 2024Quote: ... M and Enn protein constructs composed of their N-terminal 100 amino acids were purified only on Ni2+-NTA resin (Thermofisher Scientific) with the size exclusion chromatography step omitted ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified proteins were chemically labelled at the N-terminus with TFP-AlexaFluor-488 and/or AlexaFluor-568 NHS ester (ThermoFisher Scientific) following the procedure provided by the manufacturer.
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bioRxiv - Developmental Biology 2024Quote: ... the exoA coding sequence was fused with a C-terminal mCherry tag followed by the N-terminal half of GFP sequence and inserted into the pIB-V5 vector (Thermo Scientific). Plasmid SOD2-cGFP was previously published 43 ...
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bioRxiv - Developmental Biology 2024Quote: Retroviral particles for the generation of DN-KASH and Delta-PP lines were generated in HEK293T cells expressing retroviral packaging plasmids (gift from N. Montserrat) and transfected using Lipofectamine™ 3000 Transfection Reagent (Invitrogen). The viral titer collected 2-days post transfection was used to infect Rex1-GFP mESCs overnight ...
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bioRxiv - Biochemistry 2024Quote: INIP and the TRR complex were labeled on N-termini using AF488 dye (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Before labeling ...
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bioRxiv - Biophysics 2024Quote: Vero cells (ATCC CCL-81) were cultured in 35 mm confocal dishes (D35C4-20-1-N) with DMEM (Gibco, Carlsbad, CA) supplemented with 5% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... HT-1080 cells with TXNRD2-v5 over-expression was lysed with NP-40 buffer with 25 mM N-ethylmaleimide (NEM, 23030, Thermo Fisher) and protease inhibitor (04693116001 ...
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2024Quote: ... LC analysis was performed using a Kinetex 1.7 µm C8 100 Å, 50 x 2.1 mm column (S/N H17-371701, B/V 5606-0131) on a Vanquish HPLC (Thermo Scientific). The column temperature was set to 40 °C ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the ACC (n = 51) and homogenized in 350μL of lysis buffer according to the PureLink™ RNA Mini Kit (Thermo Scientific, product no ...
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bioRxiv - Microbiology 2024Quote: ... N-terminal FLAG-tagged CBP/p300 cDNA was amplified by PCR and was then inserted into pcDNA3.1 (Invitrogen Thermo, Carlsbad, CA).
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bioRxiv - Plant Biology 2020Quote: ... and biotinylated using the Biotin 3′ End DNA Labeling Kit (Thermo Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 mg/ml of type I collagen (Thermo Fisher Scientific, Rochester, NY), DMEM medium ...
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bioRxiv - Cell Biology 2019Quote: ... washed 3 more times and embedded in Prolong Antifade Gold (Thermo Scientific). Stainings with Rab5 and Rab7 antibody (Cell Signalling ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-centrin-3 (1:1000; PA5-35865; Thermo Scientific, Rockford, USA), rabbit anti-acetylated α-tubulin (1:800 ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclear counter-staining was performed with Hoechst 3(3342 (NucBlue, Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... 1% [w/w] P/S [Gibco] and 1 % [w/w] Glutamax [Gibco]) supplemented with 1 μM TO-PRO®-3 (Thermo Fisher Scientific). After 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto NuPAGE 3-8 % Tris-Acetate gradient gels (ThermoFisher) prior to transfer onto PVDF membrane ...
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bioRxiv - Cell Biology 2020Quote: ... A 200 μL transfection mix containing 3 μL Lipofectamine RNAi Max (Invitrogen) and the siRNA was prepared in OptiMEM (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were then incubated for 10 minutes with Topro 3 (T3605, Thermofisher) or mounted with mounting media containing DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated for 10 minutes with Topro 3 (T3605, Thermofisher) at room temperature.