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Citations for Thermo Fisher :
751 - 800 of 7808 citations for Recombinant Human F10 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were stained with alexa-fluor tagged Lectin (Invitrogen) for analysis of cell spreading and with phalloidin (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 5 µg DNA was used per flask (in optiMEM) and 5 hours after transfection replaced by F10-DMEM media (Thermo Fisher Scientific). 48 hours post-transfection cells were washed with PBS and scraped off ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants of ZP161 expressing rat Pex14 variants tagged with N-terminal hexahistidine (His-Pex14) were isolated by transfection of pcDNAZeo-D/His-RnPEX14 variants (see below) followed by selection with Zeocin (Invitrogen), as described (Okumoto et al. ...
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Immunology 2022Quote: ... pAc5.1A/V5-His-CBF and pAc5.1A/V5-His-EF-1α were transfected into S2 cells using Cellfectin II Reagent (Invitrogen, USA), respectively ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: A pcDNA3.1/V5-His TOPO expression vector (Invitrogen) encoding the protein-coding sequence of human SM (NM_003129.4 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-His antibody (Thermo Fisher, Waltham, MA) was diluted 1:2000 in 1x iBind solution ...
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bioRxiv - Biochemistry 2020Quote: ... into pT7-N-His-GST (Thermo Fisher Scientific) or pET28a (EMD Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
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bioRxiv - Biochemistry 2021Quote: ... then cloned into pIB/V5-His vector (Invitrogen), fused with V5 epitope at the C-terminus ...
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bioRxiv - Biophysics 2020Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti His Alexa Fluor 555 (Invitrogen catalogue number MA1-135-A555 ...
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bioRxiv - Biochemistry 2021Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Cancer Biology 2020Quote: ... in 1X Hi-Fi PCR buffer (Invitrogen #52045) supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001 ...
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bioRxiv - Genetics 2021Quote: ... and Hi - Di™ Formamide (Applied Biosystems, USA). The DNA (PCR products ...
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bioRxiv - Microbiology 2020Quote: ... MprA was detected using Anti-His antibody (Invitrogen) and anti-mouse HRP-labelled secondary antibody (Santa Cruz) ...
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bioRxiv - Immunology 2022Quote: ... 10% hi-FBS and 1% penicillin/streptomycin (ThermoFisher) in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein ...
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bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
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bioRxiv - Biochemistry 2024Quote: Basal Conditioned medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: DMEM Starvation medium: DMEM Hi Glucose medium (Gibco) supplemented with 0.2%heat-inactivated fetal bovine serum (US origin ...
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bioRxiv - Biochemistry 2024Quote: DMEM complete medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: C2C12 differentiation medium: DMEM Hi Glucose medium (Gibco) supplemented with 2% Horse Serum (Gibco ...
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bioRxiv - Immunology 2024Quote: Dynabeads His-Tag Isolation and Pulldown beads (ThermoFisher) were washed once with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Hi-FBS (Thermo Fisher Scientific). For VP4 expression ...
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bioRxiv - Microbiology 2024Quote: ... anti-6*his tag (Thermo Fisher Scientific,11533923), anti-myc tag (Abcam ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) HI-FBS (Gibco), 10 U/mL penicillin and 100 U/mL streptomycin (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-His (Invitrogen, #MA1-21315, 1:1000), rabbit anti-GST (Merck,#ZRB1223,1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... anti-6x-His mouse monoclonal (Invitrogen 37-2900), anti-CD99 mouse monoclonal (Invitrogen MA5-12954) ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (v/v) HI-FCS (Gibco), 50 μM mercaptoethanol (Sigma-Alrich) ...
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bioRxiv - Genetics 2023Quote: ... 8.85 μl of HI-DI Formamide (Applied Biosystems), and the reaction mix was adjusted to 50 μl using ddH2O ...
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bioRxiv - Biochemistry 2023Quote: The plasmid pBAD/Myc-His A (pBAD) (Invitrogen) and pET28a (+ ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% HI-FBS and 2mM EDTA (Invitrogen, 15575038).
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bioRxiv - Microbiology 2024Quote: ... Hi-DiTM formamide (Fisher Scientific, 44-407-53), phosphate buffered saline ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells and MuSCs were isolated from human skeletal muscle and plated as 200 cells/µl medium (HAMS F10 + 5% FBS + 1% Penicillin-Streptomycin (cat. no.: 15140122, Gibco, ThermoFisher Scientific, MA, USA) and incubated for 48 h ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... we added recombinant mouse IFNγ (ThermoFisher), 2-deoxyglucose (Abcam) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...