Labshake search
Citations for Thermo Fisher :
651 - 700 of 8295 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The cells were transfected with shRNA clones (as mentioned in the figures and legends) using Lipofectamine LTX with Plus Reagent (Invitrogen) for a maximum of 48hrs before any further analysis.
-
bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were transduced with Tet-pLKO-Puro (with shRNAs targeting Cx31 or GFP control) followed by puromycin (2 ug/mL; Gibco) for seven days ...
-
bioRxiv - Neuroscience 2023Quote: ... control shRNA (shCtrl) and Pgbd5 shRNA (shPgbd5) (Table S5) were cloned along the hU6 promoter in a pSilencer 3.0-H1 (ThermoFisher Scientific) plasmid using EcoRI and HindIII restriction sites and substituting the original H1 promoter ...
-
bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... astrocytes were transfected with lentiviral vectors expressing shRNAs (see “Lentivirus and retrovirus production” section below) using Lipofectamine 3000 reagent (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Astrocytes were transfected with lentiviral vectors expressing shRNAs (see the “Lentivirus and retrovirus production” section below) using Lipofectamine 3000 reagent (Invitrogen), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... shRNAs fused to GFP) targeting PAN2 ORF/3’UTR using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher Scientific 11668027). Puromycin selection was initiated 48 hours post-transfection at a concentration of 1 µg/mL to generate stably integrated shRNA-mediated knockdowns ...
-
bioRxiv - Molecular Biology 2024Quote: ... The most effective target sequences were used to generate Ad-shRNAs in the Block-iT adenoviral RNAi vector (K494100, Invitrogen). The virus that silences both DYRK1A and DYRK1B simultaneously has been reported previously (13) ...
-
bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
-
bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
-
bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006, Goat anti Rat 647 Thermo Fisher Scientific a21247 ...
-
bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).
-
bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
-
bioRxiv - Genetics 2019Quote: Plasmids for microinjection were extracted using the PureLink HQ Mini Plasmid Purification Kit or HiPure Plasmid Midiprep kit (Invitrogen). Genomic DNAs were extracted from mix-staged animals with PureLink Genomic DNA Mini Kit (Invitrogen) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA pool was isolated from each replicate using automated plasmid extraction using MagJET plasmid extraction kit (ThermoFisher Scientific). Barcode diversity assay kit from Inscripta was used to construct libraries containing barcodes from plasmid pool for each sample for sequencing ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was purified using GeneJet Plasmid Miniprep kit (Thermo) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). PCR was performed according to manufacturer directions using Q5 ...
-
bioRxiv - Biochemistry 2020Quote: ... AlexaFluor 594–conjugated anti-rat (1:250, Molecular Probes) and AlexaFluor 488–conjugated anti-mouse (1:250 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and goat α-rat (62-6520, Invitrogen, 1:5000). Proteins were detected using West Pico Chemiluminescent Substrate for 20 seconds (34578 ...
-
bioRxiv - Cell Biology 2020Quote: ... and goat-α-rat 488 (Thermo Fisher A-11006) at 1:3,000 dilution ...
-
bioRxiv - Cell Biology 2020Quote: Donkey anti rat Alexa Fluor 594 (A21209, Life Technologies) and donkey anti mouse Alexa Fluor 647 (A31571 ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 488/680 (Invitrogen, CA, USA), anti-mouse Alexa Fluor 488/800 (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006] ...
-
bioRxiv - Molecular Biology 2021Quote: ... and anti-rat Alexa Fluor 546 (1:500, Invitrogen). The incubations were performed in the dark in a humidified chamber ...
-
bioRxiv - Developmental Biology 2020Quote: ... anti-rat (goat polyclonal, Thermo Fisher, A-21434, RRID:AB_2535855); Alexa Fluor 594 ...
-
bioRxiv - Immunology 2022Quote: ... rat IgG2a kappa isotype control (1:400, Thermo Scientific), armenian hamster IgG isotype control (1:400 ...
-
bioRxiv - Immunology 2022Quote: ... rat IgG2a isotype control (1:400, Thermo Fisher Scientific), rat IgG2b kappa Isotype control (1:400 ...
-
bioRxiv - Neuroscience 2022Quote: ... AF-647 goat anti-rat (Invitrogen #A21247; 1:500), Fluorescein (FITC)-conjugated donkey anti-rabbit (Jackson ImmunoResearch ...
-
bioRxiv - Microbiology 2022Quote: ... goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific), rabbit anti-mouse-Cy3 1:400 (Dianova) ...
-
bioRxiv - Neuroscience 2021Quote: ... and monoclonal rat anti-mCherry (#M11217, ThermoFisher, RRID: AB_2536611) primary antibodies in 0.1M Tris-buffered saline containing 0.5% Roche Blocking Reagent (#11096176001 ...
-
bioRxiv - Neuroscience 2021Quote: ... Goat anti-Rat A-546 (1:500, Invitrogen, #A11081), Goat anti-Rabbit A488 (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... goat anti-rat 647 (1:400, Invitrogen #A-21247), donkey anti-chicken 405 (1:200 ...
-
bioRxiv - Molecular Biology 2021Quote: ... goat anti-rat IgG (1:5000, Thermo scientific, 31470), red fluorescence donkey anti-mouse antibody Alexa Flour® 647 (1:200 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Goat polyclonal anti-Rat HRP (Invitrogen, 31470, 1:1,000).
-
bioRxiv - Molecular Biology 2019Quote: ... Alexa-555 Goat anti-Rat (ThermoFisher A21434, 1:750), Alexa-647 Donkey anti-Sheep (ThermoFisher A21448 ...
-
bioRxiv - Cell Biology 2022Quote: ... and anti-rat 647 (1:500, Thermo Fisher Scientific), were used for visualization ...
-
bioRxiv - Developmental Biology 2022Quote: ... goat anti-rat Alexa488 (Invitrogen, Cat. A11006, 1:250), goat anti-mouse Alexa488 (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-conjugated goat anti-rat IgG (Life Technologies) at a dilution of 1:1’000 in blocking buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... goat anti-rat Alexa 594 (Life Technologies 1:400) goat anti-rabbit Alexa 594 and 488 (Life Technologies ...
-
bioRxiv - Bioengineering 2019Quote: Rat primary cortical neurons (E18, Life Technologies, Carlsbad, CA) were cultured in neurobasal medium (1mL B27 ...
-
bioRxiv - Developmental Biology 2019Quote: ... rat and rabbit nonimmune IgG (Dako or ThermoFisher Scientific) at the corresponding antibody concentration to verify absence of unspecific antibody binding ...
-
bioRxiv - Neuroscience 2019Quote: ... Goat anti-rat Alexa Fluor 568 (1:200, Invitrogen); Goat anti-mouse Alexa Fluor 568 (1:200 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Rat monoclonal E-cadherin antibody (Thermo Fisher, 13-1900) was used at 1 µg/mL for all other E-cadherin immunostaining and at 0.5 µg/mL for Western blotting ...
-
bioRxiv - Pathology 2020Quote: ... The rat ACTB (Rn00667869_m1; Applied Biosystems, Foster City, CA) endogenous control was used for normalization.
-
bioRxiv - Cell Biology 2019Quote: ... goat anti-rat IgG Alexa568 (Cat#A11077, Molecular Probes); goat anti-mouse IgG Cy3 (Cat#115-166-003 ...
-
bioRxiv - Cell Biology 2019Quote: ... goat anti-rat IgG Alexa488 (Cat#A11006, Molecular Probes); goat anti-rat IgG Alexa568 (Cat#A11077 ...
-
bioRxiv - Genetics 2019Quote: ... and Alexa Fluor 555 goat anti-rat (ThermoFisher, A21247).