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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μg/L of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (16) ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant human PDZD8 (1-28) - FLAG was expressed in Expi293 Cells (Thermo Fisher Scientific) using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated proteins were coupled to FITC-labeled 1um FluoSpheres NeutrAvidin-labeled Microspheres (Thermo Fisher) at 1 ug to 1 ul ratio of protein to beads for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were stained by incubating cells with Alexa Fluor 647-conjugated streptavidin (ThermoFisher). Coverslips were prepared for imaging by mounting in Vectashield liquid mounting media (Vector Labs).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were pulled down with High Capacity NeutrAvidin-Agarose beads (Thermo Scientific, 29202) and analyzed by immunoblotting for S-nitrosylated ACE2 ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated with the Biotin-XX Microscale Protein Labeling Kit (Life Technologies, NY, USA). 0.25 μg of biotinylated Ag or ~0.16 μg of BSA (used as a baseline control in an equivalent number of Ag molecules / bead ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed and biotinylated proteins immunoprecipitated with streptavidin-conjugated agarose beads (ThermoFisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Plant Biology 2023Quote: ... and all biotinylated proteins were bound by PierceTM streptavidin agarose beads (Thermo Fisher Scientific). To this end ...
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bioRxiv - Immunology 2023Quote: ... which we biotinylated in-house using the Biotin-XX Microscale Protein Labeling Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Biotinylated proteins were detected with streptavidin Alexa Fluor 546 conjugate (Molecular Probes; S-11225).
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were isolated from the extract using MyOne Streptavidin C1 Dynabeads (Thermo Scientific). To this end ...
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bioRxiv - Immunology 2022Quote: ... The spike protein was biotinylated using EZ-Link NHS-PEG4-Biotin reagent (Thermo Scientific) as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Vγ9Vδ2 TCR were biotinylated using protein labeling kit according to the manufacturer’s instructions (Thermofisher). Biotinylated proteins were then tetramerized with streptavidin-APC (Bio-legend ...
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bioRxiv - Systems Biology 2023Quote: The enrichment of biotinylated proteins was automated with the KingFisher Flex (Thermo Fisher Scientific). Supernatants were incubated at 4 °C for 18 hrs with magnetic streptavidin beads (Pierce™ Streptavidin Magnetic Beads ...
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bioRxiv - Neuroscience 2024Quote: Synthetic biotinylated proteins were attached to Streptavidin coupled Dynabeads M-280 (11205D, Life Technologies) in binding buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-Biotin (Thermo Scientific, 21217). Media was aspirated from wells and cells rinsed with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were eluted from the beads with 10 U of TEV protease (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... The concentration of the purified recombinant proteins was determined using the BCA protein assay kit (Thermo Scientific), with bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) with bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (Thermo Fisher Scientific), then buffer-exchanged into PBS and concentrated using Amicon Ultra centrifugal filters (MilliporeSigma) ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were expressed and purified using Expi293F cells (Life Technologies,) as described in detail previously [34 ...
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bioRxiv - Molecular Biology 2021Quote: ... The guide RNAs and recombinant Cas9-NLS protein (Thermo Fisher Scientific, B25642) were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The yield of the recombinant protein was estimated by Qubit 2.0 (Invitrogen) and the degree of purification by SDS-PAGE analysis.
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bioRxiv - Neuroscience 2020Quote: ... resuspended in water and pre-cleared with recombinant protein G agarose (Invitrogen). Immunoprecipitation of lysates was performed after lysis in NP-40 lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... The proteins were acetone precipitated prior to digestion with recombinant trypsin (ThermoFisher) (1:50 wt/wt. ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were expressed using the Expi293 Expression system (ThermoFisher Scientific) and purified with HisTrap FF columns (for polyhistidine-tagged spike proteins ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing recombinant antibody was incubated with protein A resin (ThermoFisher) overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... Recombinant CtNAT10 protein was expressed in both Sf9 cells (ThermoFisher, cat #12659017) and homemade bGroESL cells (See Experimental Model and Subject Details) ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...