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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Human PIGV shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and human plasma fibronectin (Life Technologies) coated plastic culture plates or Ø 0.4 μm or Ø 3.0 μm pore Transwell inserts (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... human (Thermo Fisher Scientific, cat# 4351379) using an Applied Biosystems™ QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The GLOBINclear Human Kit (ThermoFisher Scientific) was then employed to deplete human globin mRNA in all samples ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human FoxP3 EF450 (Invitrogen 48477642). Cells were stained with viability dye Zombie NIRtrade Fixable Viability Kit (Biolegend Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human BMP4 (Thermo Fisher Scientific) at 10 ng/ml was added every third day in the presence of EGF and bFGF in the media ...
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bioRxiv - Neuroscience 2024Quote: ... human embryonic kidney 293A cells (Invitrogen) were transfected with pHelper ...
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bioRxiv - Microbiology 2024Quote: ... Heat stable recombinant human bFGF (Gibco) at 5U/ml was supplemented to organoid formation media only on day 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid isolation was performed with the PureLink HiPure Plasmid Filter Maxiprep Kit (Thermo Fisher Scientific). Insertion was checked by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... The plasmids were isolated using the PureLink® Quick Plasmid Miniprep (Life Technologies Corporation, USA) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and plasmids were isolated from these bacterial cells using GeneJET plasmid miniprep kit (Thermo Fisher). Sequences of CHST-encoding plasmids were verified through Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... with SARS-Cov-2 spike plasmid or a control plasmid using Lipofectamine 2000 (Life technologies) are kind gifts from Olivier Schwartz ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids containing the gene of interest were extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher) and confirmed by sequencing.
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bioRxiv - Immunology 2021Quote: ... plasmids were grown under ampicillin selection and purified using GeneJET plasmid Midiprep Kit (Thermo Scientific), following the protocol specified by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and plasmid DNA was extracted using the GeneJET plasmid miniprep kit (Thermo Scientific, Waltham, MA). Plasmid DNA was sent to Eurofins Genomics for sequencing using a plasmid-specific primer included with the CloneJET kit.
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bioRxiv - Cell Biology 2021Quote: ... or a Sf3b1-HRE-luciferase reporter plasmid together with different plasmids using lipofectamine 2000 (Invitrogen). A renilla luciferase plasmid was co-transfected for internal control ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids were purified using a Plasmid DNA Midiprep Kit (K210005, Thermo Fisher Scientific, MA, USA).
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bioRxiv - Physiology 2022Quote: ... The plasmids were then extracted and purified using a PureLink Plasmid Mini Kit (Thermo Fisher) and sequenced (Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids were isolated by using Gene JET plasmid miniprep kit (Thermo Scientific, USA). The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad) ...
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bioRxiv - Microbiology 2019Quote: ... Plates were scraped and transformant plasmids isolated using the GeneJET plasmid purification kit (Thermo scientific).
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bioRxiv - Synthetic Biology 2020Quote: Yeast plasmid DNA was extracted using the ChargeSwitch Plasmid Yeast Mini Kit (Invitrogen, California, USA) by following the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was extracted from bacteria using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) and the obtained concentration was determined by Nanodrop (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid isolation was performed with the PureLink HiPure Plasmid Filter Maxiprep Kit (Thermo Fisher Scientific). Insertion was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). Oligonucleotide primers (Supplemental Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using the GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific, Landsmeer, The Netherlands). PCR products were purified from gel using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNAs used in transfection experiments were purified using the Purelink Plasmid Midi Kit (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... and the plasmids were then extracted using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific). Plasmids were Sanger sequenced on a capillary 3130xL Genetic Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Plasmid DNA was extracted using the GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific; Cat. No. K0491) for transfections ...
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bioRxiv - Cell Biology 2024Quote: ... by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen). Cells were grown for 3-5 days before selection with 25µg/ml blasticidin ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pLL3.7m-based plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then HEK293T (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... The modified pGL4.13 plasmids and pRL-CMV plasmid were co-transfected with Lipofectamine 2000 (Invitrogen) at a mass ratio of 30:1 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared from single colonies using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... with CTL plasmids plus psPAX2 and VSV-G packaging plasmids using Lipofectamine 2000 (Invitrogen 11668019). The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media ...
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bioRxiv - Synthetic Biology 2023Quote: ... Constructed plasmids were extracted using the GeneJET Plasmid Miniprep Kit® (Thermo Fisher Scientific©). All procedures followed the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... These two plasmids and a plasmid conferring puromycin resistance were transfected using LipofectamineTM 3000 (Invitrogen). Cells were selected with 1 μg/ml puromycin (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... High-purity plasmid DNA was prepared using PureLink HiPure Plasmid Midiprep Kit (Thermo Fisher Scientific). Arabidopsis mesophyll protoplast transfection was performed by a polyethylene glycol (PEG)-calcium transfection method (49) ...
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bioRxiv - Microbiology 2024Quote: ... the plasmid was isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, MA, USA). Once obtained ...
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bioRxiv - Genetics 2021Quote: Human neural stem cells derived from H9 (WA09) human embryonic stem cells were purchased from Thermo Fisher. The cells were cultured in complete StemPro NSC SFM (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: NSChIPS (Human Neural Stem Cells derived from the human induced pluripotent stem (iPS) cell line) (ThermoFisher, #A3890101)
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bioRxiv - Genomics 2019Quote: ... a mixture of 23 normal human tissues (including brain) and First Choice Human Brain reference RNA (Ambion), a pool of normal human brain tissues (labeled B) ...
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bioRxiv - Immunology 2022Quote: ... The human codon-optimized Bcl6-P2A-human codon-optimized Bcl-xL insert was synthesized using GeneArt (ThermoFisher) and cloned into pLZRS-IRES-GFP ...
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bioRxiv - Immunology 2020Quote: ... Fresh primary human T cells were isolated and cultured with anti-human CD3/CD28 beads (Dynabeads, ThermoFisher), 500U/mL IL-2 (Proleukin ...
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bioRxiv - Molecular Biology 2021Quote: Human adipose-derived stem cells (ASCs) and human microvascular endothelial cells (HMVEC) were purchased from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (NY, USA). MC was cultured in Medium
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN]) were used for cytokine quantification.
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bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Human and non-human primate OBs were sectioned following embedding in optimal cutting temperature (O.C.T.) compound (ThermoFisher). One human non-synucleinopathy case was obtained from banner health embedded in paraffin wax (Specimen details see Table 1 and SI Appendix Table S1) ...
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bioRxiv - Immunology 2023Quote: Primary human T cells were pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron #53905-991-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Physiology 2024Quote: Human: Total RNA was extracted from human RV tissue using TRIzol (Ambion by Life Technologies, Ref#15596018). cDNA was prepared using qScript Flex cDNA Synthesis Kit from (Quanta Bio ...