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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... After 20 min incubation cells were washed with PBS and unspecific bindings were saturated with 20% normal rabbit serum (Life technologies). Following 20 min incubation at 4°C cells were washed with PBS and stained with SARS-CoV-2 S-protein labeled with Strep-Tactin®XT DY-488 (iba-lifesciences cat# 2-1562-050 ...
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bioRxiv - Cell Biology 2021Quote: RBM20 WT and RM20-R636S direct transcript binding sites were determined using the same RBM20 polyclonal antibody (ThermoFisher, Cat#PA5-58068) on WT or R636S HMZ WTC iPS CMs using the eCLIP protocol as previously described (Van Nostrand et al. ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS + 1% BSA +1% goat serum + 0.05% Tween Specific binding was detected with Goat anti-Rabbit IgG-AF488 (Invitrogen, 1:1000) for 1h at 37 ◦C while nuclei were stained with DAPI (1:1000) ...
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bioRxiv - Biochemistry 2022Quote: The peptide-coding regions of the naive RiboVD library and the binding-enriched phage pools (5 µL) were PCR-amplified and barcoded using Phusion High-Fidelity polymerase (Thermo Scientific) for 22 cycles ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... in amounts ranging from 750 pM to 7.5 μM in Binding Buffer (see above) in black Nunc 384-Well Polystyrene microplates (ThermoFisher Scientific 262360) at a final reaction volume of 80 μL ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL, Thermo Fisher Scientific) and Kodak autoradiography film ...
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bioRxiv - Cancer Biology 2022Quote: ... 250uL of strained sample (5% total marrow) was centrifuged at 600g for 6 minutes and resuspended in RNA Lysis/Binding Buffer (mirVana miRNA kit, Thermo Fisher) and stored at −80C ...
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bioRxiv - Bioengineering 2022Quote: ... was coated at 2 μg/ml in phosphate buffered saline (PBS) (25 μg per well) on Corning 96-well high binding flat-bottom half-area microplates (Fisher Scientific) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: The Fab fragments for anti-SARS-CoV-2 antibodies ION_300 and ION_360 and SARS-CoV-2 receptor binding domain (RBD) were expressed in Expi293F™ cells (Thermo Fisher, A14527). The antibody:RBD complexes were mixed and co-purified by size exclusion chromatography in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... were manually excised using 21G needles/scalpel blades and grown in low-binding 96 well plates (96 well Nunc Sphera Round Bottom Plates, ThermoFisher Scientific) in Retinal Differentiation Media (DMEM/F12 nutrient mix (3:1 ratio ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody binding was visualized using species specific fluorescent secondary antibodies conjugated to Alexa Fluor goat anti-mouse 488 (1:1000; Molecular Probes). Sections were mounted using fluorescent mounting medium (DAKO).
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bioRxiv - Cancer Biology 2021Quote: ... GFP+ cells were FACS-sorted using a BD FACSAriaTM IIu in Lysis/Binding Buffer for Dynabeads™ mRNA Purification Kits (Invitrogen), and samples were snap-frozen and kept at −80 C until MARS-seq was performed.
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bioRxiv - Bioengineering 2020Quote: ... Fresh 200 μl of binding buffer were added and 1 μl of PI before flow cytometry was performed (Thermo Fisher Scientific). The excitation/emission for Annexin V is 494/518 ...
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bioRxiv - Developmental Biology 2022Quote: ... iMeLCs were then plated at 3,500 cells per well into a low-cell-binding V-bottom 96-well plate (Thermo Fisher Scientific) containing GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained with 100 μg/ml of propidium iodide and 1X annexin-binding buffer following the protocol from Dead Cell Apoptosis Kit (Invitrogen V13242). Stained cells were imaged with the Countess II FL Automated Cell Counter (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Interactions were visualized by the incubation of tetramethylrhodamine (TRITC) labelled secondary goat anti-mouse IgG antibodies (Fc specific; 1:1000 dilution in binding buffer; Life Technologies) and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... quencher dye NFQMGB (Non-Fluorescent Quencher Minor Grove Binding)) were designed using Primer Express Software (Thermo Fisher Scientific, Carlsbad, California, USA). The 123 base pair amplicon was entered into BLAST (NCBI ...
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bioRxiv - Immunology 2020Quote: ... 50 μl serially two-fold diluted pMHC were added to each well of high-binding capacity of Streptavidin-coated 96-well plates (15500, Thermo Fisher). After a minimum 45 min incubation at room temperature ...
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bioRxiv - Immunology 2020Quote: ... infected erythrocytes in binding buffer (10mM HEPES, 150mM NaCl, 2.5mM CaCl2, pH 7.4) were stained with Vybrant Violet (Thermo Fisher, V35003, 2.5μM) and ethidium bromide (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Plates washed with PBS-Tween (PBS-T) and non-specific binding was blocked with TBS Startblock blocking buffer (Thermo Fisher Scientific). Serum samples were serially diluted 3-fold starting with a 1:50 dilution and added to the coated plates ...
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bioRxiv - Biophysics 2020Quote: ... the binding reactions was centrifuged at 1000 RCF for 3 minutes and the pellet was washed with Ag/Ab binding buffer (Thermo Scientific) three times ...
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bioRxiv - Bioengineering 2021Quote: ... biotin-streptavidin binding was used to functionalize magnetic particles (Dynabeads MyOne™ Streptavidin C1, Cat. #65001, Life Technologies, Carlsbad, CA, USA) with antibodies for CTXII (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryoid body-like aggregates were formed by adding 5,000 cells/dish in a low binding 96-well microplate (174925, Nunclon Sphera, Thermo Fisher Scientific) in 100 µL of differentiating medium ...
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bioRxiv - Microbiology 2022Quote: ... 1 nM biotin-labeled DNA and varying concentrations of either CBUA0028 or CBUA0027-CBUA0028 in 1X Binding Buffer (LightShift Chemiluminescence kit; Thermo Scientific), were assembled and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: Peptide-coding regions were PCR-amplified and barcoded from the binding-enriched phage pools (5 μL) using Phusion High-Fidelity polymerase (Thermo Scientific). PCR products were normalized using Mag-bind Total Pure NGS ...
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bioRxiv - Cell Biology 2024Quote: ... to block non-specific RNA binding in 0.15M KCl Wash Buffer supplemented with 0.5mM DTT and 40U/ml RiboLock RNase inhibitor (Thermo Fisher Scientific, USA). Final washes were performed three times in 0.35M KCl Wash Buffer (10mM HEPES ...
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bioRxiv - Pathology 2024Quote: The binding affinity of Gremlin-1 or Gremlin-1-mAb mixtures to fluorescein-labelled Heparin (Heparin-FL, H7482, Thermo Fisher Scientific) was measured in a fluorescence polarization assay ...
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bioRxiv - Immunology 2023Quote: Peripheral blood or lung cells were isolated as previously described and sorted into 30 ul of lysis/binding buffer (home-made or Life Technologies) and stored at −80°C ...
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bioRxiv - Immunology 2023Quote: ... 105 or less (when isolated cell number was insufficient) microglia and leptomeningeal MΦ were sorted into 40 µL of lysis/binding buffer (Life Technologies) and stored at −80 °C until mRNA isolation ...
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bioRxiv - Immunology 2023Quote: Dried peptides were re-suspended in 50 µL Fe-NTA binding buffer (0.1% TFA, 80% acetonitrile; included in High-SelectTM Fe-NTA Phosphopeptide Enrichment Kit, Thermo Scientific #A32992).
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bioRxiv - Microbiology 2023Quote: ... Samples were subsequently incubated with 10 % BSA to block the unspecific binding followed by incubation with a secondary α-rabbit antibody conjugated to Alexa Fluor 488 (Invitrogen) and diluted 1:1,000 (in PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Each slice was placed into a 2ml low binding tube (DNA LoBind® #0030108078, #0030108051) containing 150ul Trizol (Invitrogen™ #15596026) and a 5mm stainless steel bead (#69989 ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Immunology 2023Quote: ... quantification of ADCP was performed by covalently binding SARS-CoV-2 NTD and NTD_ADEm3 to NeutrAvidin fluorescent beads (ThermoFisher, Waltham, MA). Immune complexes were formed by incubation with serially diluted (2 fold ...
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bioRxiv - Cell Biology 2022Quote: ... A column for affinity purification was prepared by binding the peptide to a SulfoLink resin column using the SulfoLink Immobilization Kit (Thermo Fisher). Antibodies were affinity-purified from serum using this column.
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bioRxiv - Cancer Biology 2023Quote: ... counted and re-suspended in Annexin binding buffer from the Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit (Thermofisher) and followed manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001) by rotating the tube for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then transferred to new low-binding 1.5 mL tubes and 0.75 μL glycogen (RNA grade, Fisher Scientific, cat#: FERR0551), 40 μL 3 M sodium acetate (pH 5.5) ...
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bioRxiv - Biochemistry 2023Quote: ... To carry out filter-binding experiments, nitrocellulose (Amersham Protran, 0.45 µm pores; Cytiva, 10600062) and nylon (Amersham Hybond-XL; Fisher Scientific, 45001147) membrane filters were cut into discs of 0.5 inch diameter and equilibrated at 25°C for at least 20 min in filter-binding buffer (18 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... RBDs or NTD was performed by coating Nunc MaxiSorp flat-bottom high-binding 96-half-well plates (ThermoFisher Scientific, MS, USA) with 50 μl per well of a 0.1 μg ml−1 protein solution in PBS overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: Antigens for differential phage panning or antibody binding in IgG format were expressed by transient transfection of BirA-Expi293 cells (Life Technologies) with plasmids encoding CDCP1 ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed with phosphate-buffered saline 1X (PBS) and unspecific bindings were saturated with 50 µL of 20% normal rabbit serum (Life Technologies) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 104 cells from the microglia population was sorted into 40-200 µL of lysis/binding buffer (Life Technologies). mRNA was captured with 12 µl of Dynabeads oligo(dT ...
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bioRxiv - Cancer Biology 2024Quote: ... and MSCV KSR2 FIF570 IRES YFP resistant to binding hairpin sh5 (FIF570r) were transfected into HEK-293T cells using trans-lentiviral packing system (ThermoFisher Scientific). The virus was collected 48 hours post transfection and used to infect KP1 sh5 cells with 8 µg/mL Polybrene for 72 hours ...
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bioRxiv - Immunology 2024Quote: ... were diluted in PBS and applied to wells of a 384-well high-binding polystyrene Nunc plate (Thermo Fisher Scientific, #460372) at a final amount of 50 ng/well ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were determined using Pierce 660 nm protein assay (Thermo Scientific). Extracts with 500 μg of protein were alkylated with 14 mM chloroacetamide (CAA ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration was measured by EZQ protein quantification kit (Thermo Fisher Scientific), and 30 µg of protein from each sample was reduced with 10 mM dithiothreitol overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured by BCA Protein assay kit (Thermo Fisher Scientific) at 562 nm using BSA to produce a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) and purity was checked by sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ...