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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: Human myeloma cells MM1.S (ATCC: CRL-2974) were cultured in RPMI 1640 cell media (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2024Quote: ... the mean fluorescence intensity (MFI) of Alexa488-coupled goat anti-human IgG (1:500; Life Technologies) was evaluated ...
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bioRxiv - Microbiology 2023Quote: Human hepatoma (HepG2) cells were cultured in complete Dulbecco’s Modified Eagle Medium (cDMEM) (Gibco 10313-021) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... we isolated human neutrophils from whole blood and stained cells with Calcein AM (C1430, Thermofisher Scientific) at 4 °C for 1h in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by probing with Goat anti-human IgG secondary antibody with HRP conjugated (Invitrogen 62-8420) for 1 hour 37℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... A549 human lung carcinoma cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 2mM L-Glutamine (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: Silencing RNA for NCL (human NCL silencer select) and siRNA controls were obtained from Thermo Fisher. For siRNA-mediated silencing ...
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bioRxiv - Cancer Biology 2023Quote: ROS were detected in human NSCLC lines using the cell permeable fluorophore CellROX Green (ThermoFisher Scientific). Cells were seeded in 6-well plates densities stated above in 2 mL media 24 h prior to performing the assay ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 105 OCSC1-F2 human OC cells were resuspended in serum-free Opti-MEM (Thermo Scientific) and seeded on trans-well inserts with a polyethylene terephthalate membrane pore size of 8.0 μm (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Human peripheral blood was collected from healthy adult donors in sodium citrate vacuum tubes (Fisher Scientific). Neutrophils were isolated by negative magnetic bead selection using MACSxpress Neutrophil Isolation Kit ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney (HEK) 293T cells (293T) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended in complete human embryonic stem cell (hESC) medium consisting of DMEM/F12 (11320033, ThermoFisher), 20% (v/v ...
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bioRxiv - Biochemistry 2023Quote: Human MCF10A breast epithelial cells (ATCC CRL-10317) were cultured in DMEM/F-12 (Gibco 11330032) with 5% horse serum (Gibco 16050122) ...
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bioRxiv - Biophysics 2023Quote: Human and primary mouse myotubes were grown and maintained in DMEM-F12 (Gibco: Waltham, MA, USA) supplemented with 20% fetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: Normal human PBMCs were seeded on a Nunclon Sphera-Treated 24-well plate (Thermo Fisher Scientific) at 1.0×106 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... gondii tachyzoites were cultured in human foreskin fibroblasts in Dulbecco’s modified Eagle’s medium (Gibco: 11960-051) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated with a goat anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific). For IgG subclasses and IgM detection ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GPIX antibodies (5 µg/ml) and AlexaFluor647-labeled human fibrinogen (Life Technologies, 100 µg/ml) were added to WB ...
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bioRxiv - Cell Biology 2024Quote: ... Samples with 1×106 isolated human platelets were prepared in binding buffer (Invitrogen, Carlsbad, CA, USA). Hemin and CPR-XL were incubated at the indicated concentrations for 1h at room temperature.
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bioRxiv - Neuroscience 2024Quote: Human primary Müller cells (P3) after siRNA treatments were incubated with JC-1 dye (#T3168, Invitrogen) at a concentration of 1.0 μg/mL in DMEM at 37 °C for 30 minutes to measure the mitochondrial membrane potential ...
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bioRxiv - Immunology 2024Quote: Freshly isolated CD8+ cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) at a cell/bead ratio of 1:0.5 for 48 h in conditioned media obtained by combining R10 medium and culture supernatants derived from either leukemic or healthy B cells (1:1 ratio) ...
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bioRxiv - Bioengineering 2024Quote: Cryopreserved human primary hepatocytes (post-thaw plateability with >90% viability) were purchased from Thermo Fisher (Hu1960). Hepatocyte donor information is available in supplementary Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... Six µg/mL of goat anti-human H+L conjugated Alexa 488 (Thermo Fisher Scientific A11013) together with 8 µM of antifade-46-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2024Quote: Human brain tissue was obtained at autopsy and immersion fixed in 10% formalin (Thermo Fisher Scientific) at 4°C for 1-3 days ...
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bioRxiv - Cell Biology 2024Quote: ... PC3 cells were transfected with cDNA encoding human talin-1 (NM_006289) using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2024Quote: PXR siRNA (siRNA NR1I2 silencer human, s16909, Life) transfection experiments were performed using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK-293T) ASIC-KO cells25 were maintained in Minimum Essential Medium (Gibco #11095) supplemented with 10% EqualFetal Bovine Serum (Atlas Biologicals #EF-0500-A) ...
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bioRxiv - Cell Biology 2024Quote: ... Human MCF10A breast epithelial cells were cultured in DMEM/F12 (DMEM/Nutrient Mixture F-12, Gibco) supplemented with 5% fetal horse serum (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: Cystatin A concentration was assessed using a Human Cystatin A ELISA kit (Invitrogen, catalog no. EH140RB). Total cell lysates were extracted from CAR T cells using radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Biochemistry 2024Quote: ... which were co-transfected into human U251-MG glioblastoma cells (ECACC 09063001) using Lipofectamine 3000 (ThermoFisher). Transfectants were selected for three days at which point selection antibiotic was removed ...
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bioRxiv - Immunology 2024Quote: ... and containing recombinant human stem cell factor (h-SCF; Gibco, PeproTech, #300-07; 100 ng/mL) and human IL-6 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated with goat polyclonal anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Caco-2 (human, intestine; HTB-37, ATCC, RRID: CVCL_0025) were cultivated in minimum essential medium (GIBCO) supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2024Quote: ... The human GBA1 sequence was detected using TaqMan Gene Expression Assays (Thermo Fisher Scientific Inc., Hs00986836_g1). The primer/probe sequences used for the detection of beta actin ...
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bioRxiv - Neuroscience 2024Quote: ... FAM labelled probes used during amplification included probes targeting Human SOD1 and Mouse HPRT (Applied Biosystems). Gene expression was calculated using the ΔΔCT method.
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) were cultured in Dulbecco’s Modified Eagle Medium from (Invitrogen, cat# 11965-118) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: The human Burkitt lymphoma cell line (BJAB) was cultured in Dulbecco’s modified Eagle medium (DMEM,GIBCO) supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected with plasmids using 293fectinTM Transfection Reagent (Cat: 12347019, Life Technologies) when the cell density reached 1 × 106 cells/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were transfected with the plasmid pSpCas9-UPP1 using Lipofectamine 3000 Reagent (Invitrogen, L3000001) in the following proportion ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transiently transfected with plasmids with use of transfection reagent of Lipofectamine 2000 (Invitrogen), PolyFect (QIAGEN) ...
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bioRxiv - Cell Biology 2020Quote: Plasmids of interest were transfected using Lipofectamine 3000 and the P3000TM Enhancer Reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Molecular Biology 2021Quote: ... NM_003286.3) with its starting ATG and termination codon was cloned into plasmid pYES2.1/TOPO (Invitrogen). The resulting clone pYES2-TOP1 expresses recombinant human TOP1 under the control of the GAL1 promoter ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with pcDNA-4mtD3cpv plasmid using Lipofectamine® 3000 Transfection kit (Thermo Fisher). 24 h after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with GFP labelled LCK CRISPR/Cas9 plasmid using lipofectamine 3000 (Thermo Scientific) in the presence of antibiotic-free ...
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bioRxiv - Cancer Biology 2022Quote: Myc-tagged LCK containing plasmid was generated using pENTR/D-TOPO cloning kit (Thermo Scientific) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the cDNAs for LRRK2-WT and LRRK2-G2019S were isolated from plasmids (Invitrogen, California, USA) for subcloning into LexAop plasmids (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNAs were quantified using the NanoDrop ND-1000 UV-Vis spectrophotometer (Thermo-Fisher Scientific), after which the DNA was precipitated and subsequently dissolved in Opti-MEM™ Reduced Serum Medium (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and 5 µg Bmal1:luc or Per2:luc plasmid constructs using Lipofectamine3000 (Thermo Fisher Scientific). After 48 h incubation ...