Labshake search
Citations for Peprotech :
1 - 50 of 668 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Differentiation supplements: BDNF (PeproTech, Cat# 450-02), CNTF (PeproTech ...
-
bioRxiv - Neuroscience 2023Quote: ... Then NPCs were seeded on Matrigel®-coated flasks at 50 000 cells/cm2 and kept in Astrocyte Induction medium (DMEM/F-12 supplemented with 1x N2 supplement, 1x B27 supplement, 10ng/ml FGF-2 (PeproTech) and 10ng/ml EGF (Miltenyi) ...
-
bioRxiv - Microbiology 2024Quote: ... All supplements were obtained from Peprotech (New Jersey, USA). except ROCK inhibitor which was purchased from Tocris (Minneapolis ...
-
bioRxiv - Neuroscience 2021Quote: ... + N2 Supplement-A + 20 ng/ml recombinant human BDNF (Peprotech) + 20 ng/ml recombinant human GDNF (Peprotech ...
-
bioRxiv - Neuroscience 2021Quote: ... + N2 Supplement-A + 20 ng/ml recombinant human BDNF (Peprotech) + 20 ng/ml recombinant human GDNF (Peprotech ...
-
bioRxiv - Neuroscience 2024Quote: ... Differentiation supplement SVZ and SGZ: BDNF (PeproTech, Cat# 450-02). The differentiated cultures were fixed after 30 days in differentiation media ...
-
bioRxiv - Bioengineering 2022Quote: ... The supplement mix consisted of EGF (50 ng ml−1; Peprotech), N2 supplement (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®; 10 ng/ml FGF-2, PeproTech; 10 ng/ml EGF, Miltenyi). NPC were then transduced with a VSV-G-pseudotyped lentivirus (carrying pCW57.1 plasmid from Addgene # 41393 (gift from David Root ...
-
bioRxiv - Neuroscience 2023Quote: ... 40 ng/ml IGF-1 (all supplements from Peprotech, London, United Kingdom). Medium with all supplements is called Expansion Medium.
-
bioRxiv - Cancer Biology 2022Quote: ... Pro/preB cell cultures were propagated with 10 ng/mL rmIL-7 (217-17, Peprotech) in RPMI-1640 medium complete (R8758 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cell culture medium was supplemented with 5 ng/ml recombinant human TGF-β (Peprotech), 10 µg/ml anti-IL-4 antibodies (clone 11B11) ...
-
bioRxiv - Immunology 2024Quote: ... CD4+ cell cultures were treated with recombinant IFNγ (50 ng/ml; Peprotech, Cranbury, NJ, USA) and survivin inhibitor sepantronium bromide (Nakahara et al. ...
-
bioRxiv - Immunology 2022Quote: ... and pro-fibrotic supplements M-CSF (25 ng/ml, Peprotech, Rocky Hill, NJ) and IL-13 (50 ng/ml ...
-
bioRxiv - Immunology 2023Quote: ... For B cell cultures, CD40L-trimer (2 nM, EnzoLifeScience) and IL-21 (100 ng/mL, Peprotech) were added ...
-
bioRxiv - Immunology 2019Quote: ... Culture supernatants or standards (Peprotech) were incubated for 2 h at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... as were pro-fibrotic supplements M-CSF (25 ng/ml, Peprotech, Rocky Hill, NJ) and IL-13 (50 ng/ml) ...
-
bioRxiv - Developmental Biology 2020Quote: ... These transfected cells were mixed with 2 ml culture medium (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Culture media for SKNO-1 cell line was supplemented with human GM-CSF (10 ng/mL, PeproTech). Cells tested negative for Mycoplasma by the MycoAlert PLUS Mycoplasma Detection Kit (Lonza) ...
-
bioRxiv - Bioengineering 2024Quote: ... T-cell cultures were supplemented with 200 U/mL IL-2 (Cat # 200-02, Peprotech (Thermo Fisher), Cranbury ...
-
bioRxiv - Neuroscience 2023Quote: ... to the medium from day 0 supplements were added: SHH C25II (100 ng/ml, Peprotech), Purmorphamine (2 μM ...
-
bioRxiv - Immunology 2021Quote: ... Lin-cells were culture in complete RPMI medium (cRPMI) containing 20 ng/ml of SCF (PeproTech, #250-03), TPO (PeproTech ...
-
bioRxiv - Cancer Biology 2020Quote: Murine and human CCL2 levels of cell culture supernatants were detected using appropriate ELISA kits (Peprotech; London, UK). For sample preparation ...
-
bioRxiv - Immunology 2019Quote: ... into B cell culture medium supplemented with 1 ng/mL recombinant mouse IL-4 (PeproTech Ca.# 214-14) and 2 μg/mL anti-mouse RP105 clone RP/14.
-
bioRxiv - Genetics 2021Quote: ... T cells were transferred to the culture medium supplemented with 30 IU/mL IL-2 (Peprotech, 200-02A). After electroporation for 3 days ...
-
bioRxiv - Developmental Biology 2022Quote: ... with STEMdiff™ SMADi Neural Induction Supplement (SMADi) and 10 μM Y-27632 (ROCK inhibitor, Peprotech) (or StemFlex (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... The culture medium contained 3μM CHIR99021 (Peprotech), 25ng/mL EGF (Peprotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... The culture medium contained 3µM CHIR99021 (Peprotech), 25ng/mL EGF (Peprotech) ...
-
bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
-
bioRxiv - Cancer Biology 2019Quote: ... At day 12 cell cultures were enriched with hNCCs and cultured in N2B27 supplemented with bFGF and EGF (both 20ng/ml, Peprotech) and 3 μM CHIR99021.
-
The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: After 48 hours of culture in preculture medium (complete IMDM medium supplemented with 10% FCS, stem cell factor (SCF) (100 ng/mL, Peprotech), FMS-like tyrosine kinase-3 ligand (FLT3-L ...
-
bioRxiv - Physiology 2022Quote: ... NRK-49 cells whose culture medium was supplemented with 400pg/mL of recombinant human IL-1β (PeproTech, Cranbury, NJ, USA) and 1×10-7M Angiotensin II acetate human (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
-
bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
-
bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
-
bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... Hepatocyte-like cells were made by further incubating the cultures in Stage 3a media (HCM containing 20ng/mL Hepatocyte Growth Factor (Peprotech)) for 5 days followed by a further 15 days in Stage 2b media (HCM containing 10 𝜇g/mL Oncostatin M (R&D Systems Cat No ...
-
bioRxiv - Neuroscience 2019Quote: ... The concentration of TNF in cell culture supernatants was quantified by enzyme-linked immunosorbent assay (ELISA) following the instructions provided by the manufacturer (Peprotech, UK). Absorbance at 405 nm ...
-
bioRxiv - Immunology 2019Quote: ... Lin- bone marrow cells were grown in culture with complete IMDM medium supplemented with 10 ng/ml M-CSF (Peprotech, QC).
-
bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
-
bioRxiv - Immunology 2019Quote: ... the suspension cells were removed and culture in a new flask along with 10ng/ml recombinant murine IL-3 (Peprotech, Rocky Hill, NJ). Cells were passaged twice per week ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were changed into fresh iNI media (Neurobasal with SM1 supplement, 1% Penicillin/Streptomycin, and 1% GlutaMAX) containing BDNF (10 ng/µL; Peprotech), GDNF (10 ng/µL ...
-
bioRxiv - Neuroscience 2023Quote: ... or STEMDiff Astrocyte Maturation media without Supplement B with the addition of “TIC” proinflammatory factors (30ng/mL TNF-alpha (Cat No. 300-01A-10ug, Peprotech), 3ng/mL IL1-alpha (Cat No ...
-
bioRxiv - Immunology 2022Quote: ... cultures were supplemented with IL-12 (10 μg/ml, Peprotech) and anti-TGFβ1 mAb (20 μg/ml ...
-
bioRxiv - Immunology 2023Quote: ... in low calcium homemade culture medium containing recombinant mEGF (Peprotech) + Chelex treated FCS (see Supplementary material and 15) ...
-
bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
-
bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
-
bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
-
bioRxiv - Cell Biology 2021Quote: ... Cultures were supplemented with human recombinant 50 ng/ml TPO (Peprotech), 20 ng/ml human recombinant IL-6 (STEMCELL Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... The culture was supplemented with 15 ng/mL IL21 (PeproTech, UK) to aid the induction of proliferation ...