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Citations for Peprotech :
1 - 50 of 81 citations for Serum Free RocketFuel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Medium in the lower chamber was either serum-free medium or serum-free medium supplemented with 20 ng/ml HGF (PeproTech, 100-39). Transwell migration was allowed for 18 hours ...
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bioRxiv - Immunology 2023Quote: ... serum-free medium supplemented with Stem Cell Factor (Peprotech) 100 ng/mL and IL-9 (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... and replaced with serum free media (control) or serum free media supplemented with 10 ng/ml TNF-α (PeproTech EC Ltd., London, UK) and 10 ng/ml IL-1β (R&D Systems Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... the old serum-free medium was replaced with new serum-free media with or without 100 ng/mL EGF (Peprotech AF-100-15). For each experimental condition (i.e. ...
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bioRxiv - Cancer Biology 2021Quote: ... with serum-free DMEM/F12 medium containing EGF (PeproTech, NJ, USA), basic Fibroblast Growth Factor (bFGF ...
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bioRxiv - Neuroscience 2023Quote: ... Serum-free F10 medium with or without recombinant TGFβ1 (Peprotech 100-21), conditioned media from wildtype or Col6a2-/- fibroblast cultures ...
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bioRxiv - Neuroscience 2020Quote: ... OPCs were passaged and plated onto poly-d-lysine coated glass coverslips in 24 well plates at 50,000 cells per well in SATO serum-free media containing NT-3 (1 ng/ml, PeproTech), and CNTF (10 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and the media was changed to serum-free DMEM/F12 with 1 % neomycin and 25 ng/ml BDNF (Peprotech). The cells were used from day 12 to day 18 after differentiation.
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in neurosphere conditions on non-adherent plates in serum free media supplemented with EGF (20 ng/mL; Peprotech), FGF2 (20 ng/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Purified OPCs were expanded in poly-d-lysine coated flasks for 4-5 days in SATO serum-free media containing Platelet-Derived Growth Factor-AA (PDGF-AA, 10 ng/ml, PeproTech), neurotrophin-3 (NT-3 ...
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bioRxiv - Molecular Biology 2019Quote: ... MLO-Y4 cells were treated with the following reagents in serum-free media: 5 ng/ml TGFβ1 (Peprotech, 100-36E), 0.1 μM PGE2 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and the clones with the highest efficacy were expanded and adapted for suspension growth in Pepro AF-CHO serum free media (Peprotech). Six liters of media at 5×106 cells/ml was centrifuged at 1000xg for 30 minutes and the secreted protein was purified to homogeneity as previously described (Albright et al. ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were subject to cytokines stimulation in serum free medium containing 10 ng/ml rat IFN-γ (Peprotech, #400-20) and 10 ng/ml rat TNF-α (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: ... Media was serum-free TexMACS (Miltentyi Biotech 170-076-307) supplemented with recombinant human IL2 in concentrations specified by the DOE (Peprotech 200-02). Cell cultures were expanded for 14 days as counted from the time of initial seeding and activation ...
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bioRxiv - Neuroscience 2020Quote: ... the medium was gradually changed to serum-free medium (Klenke & Taylor-Burds, 2012) supplemented with 5ng/ml HB-EGF (PeproTech, Cat# 100-47, Rocky Hill, NJ), and B27 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 ng/ml animal-free recombinant human EGF (Peprotech); 100 ng/ml recombinant human Noggin (Biotechne) ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng/ml animal-free recombinant human FGF10 (Peprotech); 50 ng/ml animal-free recombinant human EGF (Peprotech) ...
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bioRxiv - Molecular Biology 2021Quote: ... Animal-free recombinant IL-1RA (Peprotech, AF-200-01RA), mouse IL-1β (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/mL Animal-Free Recombinant Human EGF (Peprotech), 40 ng/mL Recombinant Human FGF-basic (Peprotech) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and animal-Free Recombinant Human EGF from PeproTech (Cranbury, NJ). Propidium iodide was from Invitrogen (Waltham ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated cells were plated on matrigel-coated dishes and allowed to grow in DMEM containing 20% fetal bovine serum (FBS) and 10% horse serum (HS) in the presence of 10ng/ml basic FGF and 2ng/ml HGF (Peprotech). Differentiation was induced when cells reached 70-80% confluence by culturing in DMEM containing 2% FBS and 2% HS (Differentiation medium ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with either low-serum media or low-serum media supplemented with 20 ng/mL HGF (PeproTech, 100-39) or by standard culture media supplemented with Lomustine (Selleckchem ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells were cultured in Animal-Free PeproGrow AF-CHO Media (PeproTech) in a 10 cm dish and transfected with pAAV/sCSF1R using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/ml Animal-Free Recombinant Human EGF (Peprotech; #AF-100-15), 10 ng/ml Recombinant Human FGF-basic (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/ml Animal-Free Recombinant Human EGF (Peprotech; #AF-100-15), 10 ng/ml Recombinant Human FGF-basic (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 with 50 ng/ml animal-free recombinant human IL-15 (Peprotech). For stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... 20ng/ml Animal-Free Recombinant Human Epidermal Growth Factor (Peprotech,#AF-100-15), 0.5 mg/ml Hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Animal-Free Recombinant Murine IL-4 (AF-214-14, Peprotech, Cranbury, NJ, USA) was diluted to 10 pg/μL or 100 pg/μL with saline and microinjected at 1 μL per mouse into the DRN same as the viral injection ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL animal-free recombinant human Stem Cell Factor (SCF, Peprotech, AF-300-07), and 10 µM RA ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL animal-free recombinant human Stem Cell Factor (SCF, Peprotech, AF-300-07), and 10 μM all-trans retinoic acid (RA ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS; Hyclone) and 1ng/mL FGF2 (Peprotech). After five days ...
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bioRxiv - Immunology 2023Quote: ... cells were suspended in CFM (Cytokine Free Media) media with interleukin-7 (10 ng/mL; Peprotech, NJ), and plated on 96 well U-bottom plates ...
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bioRxiv - Immunology 2021Quote: ... Serum samples (1:4 dilution) and a serially-titrated mouse IL-4 standard (Peprotech) were added and incubated for 2h ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 5% human AB serum and 100 Units/mL human IL-2 (PeproTech). After a 24-hour incubation ...
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bioRxiv - Immunology 2021Quote: ... in antibiotic-free RPMI-1640 medium supplemented with 10% FBS and murine IL-5 (10 ng/mL, PeproTech). 108 bioluminescent C ...
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bioRxiv - Genetics 2021Quote: ... and were maintained under feeder-free conditions in StemFit Basic04 medium (Ajinomoto, Tokyo, Japan) containing basic FGF (Peprotech) at 37 °C under an atmosphere of 5% CO2 in air ...
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bioRxiv - Immunology 2023Quote: ... supplied with 5 % human AB serum (MP Biomedical) and 10ng/mL human IL-2 (Peprotech), hereafter referred to as cX-VIVO ...
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A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... Serum-starved cells were treated with recombinant human EGF (AF-100-15; PeproTech, Cranbury, NJ) at 10 ng/mL for ≤ 15 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and maintained under feeder-free conditions in StemFit® Basic04 medium (Ajinomoto) containing 20 ng/ml basic FGF (Peprotech) at 37°C under a 5% CO2 atmosphere ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then pipetted into penicillin-streptomycin free StemSpan SFEM medium with thrombopoietin (TPO; 20 ng/mL; PeproTech) and stem cell factor (SCF ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... and were maintained under a feeder-free condition in the StemFit® Basic04 medium (Ajinomoto) containing basic FGF (Peprotech) at 37 °C under an atmosphere of 5% CO2 in air ...
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bioRxiv - Neuroscience 2022Quote: ... MSNs cultured in serum withdrawal were treated for 48 h with APOE3 (PeproTech, 350- 02-500UG) at 312 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... and grown using serum-free GBM complete medium consisting in Neurobasal-A medium mixed 1:1 with DMEM/F12+GlutaMAX and complemetend with human FGF-basic and human EGF (animal free, Peprotech) at 20 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells at a plate confluence of 80% were treated for 10 min with 100 ng/mL animal-free recombinant human EGF (PeproTech) or Gibco™ distilled water (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The iPSC colonies were maintained in undifferentiated pluripotent state by culturing the cells under feeder free conditions on 0.1% Geltrex® (Peprotech) coated culture plates ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Cancer Biology 2022Quote: ... with 10% fetal bovine serum (FBS; HyClone, SH30396.03) and 40 ng/ml M-CSF (Peprotech, 315-02). An additional 15 ml of media with M-CSF was added after day three ...
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bioRxiv - Cell Biology 2021Quote: ... Each serum was assayed for murine IFN-y in duplicate using an enzyme immunoassay kit (Peprotech, BGK01580).
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bioRxiv - Cancer Biology 2023Quote: ... After 6 hours of serum starvation cells were stimulated with recombinant mouse LIF (5 ng/mL, Peprotech). Cells were lysed with Pierce RIPA buffer (Thermo Scientific ...