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Citations for Peprotech :
1501 - 1550 of 1601 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: The medium was further supplemented with growth factors and signalling modulators comprising of human Epidermal Growth Factor (hEGF) (12ng/ml, PeproTech), B27 Supplement (2% ...
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bioRxiv - Developmental Biology 2022Quote: ... These epithelial cells were then propagated in a complete medium supplemented with the addition of human Epidermal Growth Factor (hEGF) (12ng/ml, PeproTech), ROCK inhibitor (Y-27632 ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in RPMI 1640 containing 20% FBS and 50 ng/ml human macrophage colony-stimulating factor (M-CSF) (300-25, Peprotech). Cells were seeded into sterile tissue-culture plates and differentiated for 7 days ...
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bioRxiv - Neuroscience 2023Quote: ... iSNs were treated with 10% plasma or 100ng/mL recombinant human ET1 (R&D, #1160) or CCL2 (PeproTech, #300-04) diluted in iSN maturation media for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Leptin concentration within all collected samples was determined using the Human Leptin Standard ABTS ELISA Development Kit (PeproTech #900-K90) and ABTS ELISA Buffer Kit (PeproTech #900-K00 ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: SP2/O-Ag14 myeloma and primary clones of hybridomas were cultured in GIT medium (catalog no. 63725715, Wako) supplemented with recombinant human interleukin-6 (IL-6) (catalog no. 20006, PeproTech). Cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C and sub-cultured every day ...
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bioRxiv - Developmental Biology 2024Quote: ... expanded neural progenitors were re-plated at a density of 3×104 cells/cm2 in expansion media and 24 hours later switched to N2B27 supplemented with 10 ng/mL human recombinant CNTF (Peprotech) and 10 ng/mL human recombinant BMP4 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were restimulated for 5 hours at 37°C with 1 μg/ml of MHC class I-restricted LCMV peptide gp33-41 in the presence of 2 ng/ml recombinant human IL-2 (Peprotech), 10% fetal bovine serum (premium FBS ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...
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bioRxiv - Genomics 2024Quote: ... macrophages were cultured in macrophage media supplemented with 20 ng/ml human recombinant IFN-γ (PeproTech, catalog no. 300-02) and 25 ng/ml LPS (Invivogen ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Primary Keratinocytes from three donors were seeded in 24-well plates and stimulated with KGM Media supplemented either with 0.1 ng/ml or 10 ng/ml of recombinant human IFN-γ (PeproTech), or infected with T ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Bioengineering 2023Quote: ... At Day 11 the cytokine composition of the media was changed to 10ng/μl of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/μl (Peprotech). From Day 15 onwards ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 ng ml-1 recombinant human EGF (epidermal growth factor) and recombinant Noggin (100 ng ml-1) (both from Peprotech). The medium was supplemented with 0.2% Normocin (InvivoGen ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Immunology 2024Quote: ... Reprogramming under immunosuppressive cytokine conditions was performed using DMEM complete medium supplemented with human IL-6 (10-40 ng/ml; Peprotech), TGF-ϕ3 (50-200 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were assayed immediately or cultured overnight in StemSpan SFEM (STEMCELL) with 50 ng/mL recombinant human stem cell factor (PeproTech), 10-6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... On day 11 the cytokine composition of the media was changed to 10ng/ml of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/ml (Peprotech). From day 15 onwards ...
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bioRxiv - Immunology 2024Quote: ... 1×106 differentiated iRBCs or undifferentiated HUDEP-2 cells were stimulated with 50 ng/mL recombinant human interferon-gamma (PeproTech) for 6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Cell Biology 2020Quote: ... with 500 nM SB431542 (Selleckchem, cat. no. S1067) and 20 ng/ml recombinant human (rh)Noggin (Peprotech, cat. no. 120-10C). On days 5-9 ...
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bioRxiv - Cancer Biology 2019Quote: ... 20ng/mL human recombinant epidermal growth factor (EGF) and 20ng/mL basic broblastic growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ).
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bioRxiv - Biochemistry 2021Quote: Recombinant human platelet-derived growth factor-BB (PDGF-BB) and recombinant human transforming Growth Factor-β1 (TGFβ1; HEK293 derived) were purchased from PeproTech (Hamburg, Germany) and they were dissolved in sterile ddH2O ...
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bioRxiv - Immunology 2020Quote: ... containing 9 µg of encapsulated GM-CSF (Murine GM-CSF, Sanofi, USA; Human - Leukine® for pig and rabbit, Peprotech, USA) were combined with a 1 mL solution of captured antigen ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Immunology 2022Quote: ... X-Vivo 15 media was supplemented with 100 ng/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 20 ng/ml human interleukin 4 (IL-4) (both cytokines obtained from Peprotech, Germany), and cells were cultured with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... blasts were pre-treated for 48 hours with Recombinant Human IFN-γ at a final concentration of 200 U/mL (cat#300-02 from PeproTech). Cells were cultured in IMDM supplemented with 1% L-glutamine (G) ...
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μM of A83-01 (HY10432, MCE, USA) and 20 ng/mL of recombinant human epidermal growth factor (EGF; AF100-15, PeproTech, USA), 10% KSR and 1% NEAA ...
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bioRxiv - Immunology 2023Quote: EndoC-βH1 cells and dispersed human islets were exposed or not to IFNα (2000 U/mL, PeproTech Inc., Rocky Hill, NJ), IFNγ (1000 U/mL ...
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bioRxiv - Immunology 2023Quote: ... Transfected cells were incubated for 24 hours before treatment with 50 ng/mL recombinant human IFN-γ (Peprotech; cat # 300-02) or cDMEM only control for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... crosslinked following 10 min exposure to 100 mM CaCl2 and allowed to adsorb for 30 min with recombinant human vascular endothelial growth factor (rhVEGF 165, PeproTech, USA) at 100 μg mL−1 at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... then 50 µL of 24 h co-culture supernatants or serial dilutions of Recombinant Human IFNγ standard (#300-02, Peprotech, USA) were added to the wells and incubated for 2 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... The differentiation medium was composed of modified N2B27 medium supplemented with 50 ng/ml recombinant human FGF4 (Peprotech, 100-31-25), 1 μg/ml heparin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... NBP2-35892), IFNω (Novusbio, NBP2-35893), IFNγ (Novusbio, NBP2-34992), IFNλ1 (Novusbio, NBP2-34996) and recombinant human TNFα (PeproTech, 300-01A).
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...