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Citations for Click Chemistry Tools :
1 - 50 of 102 citations for 6 Amino 3 morpholin 4 ylmethyl indazole 1 carboxylic acid tert butyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 6-Azidohexanoic Acid Ester (Click Chemistry tools, > 95%), Alkyne-PEG4-NHS Ester (Click Chemistry Tools > 95%) ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Bioengineering 2021Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... was conjugated to 0.3 mM azidoacetic acid NHS ester (#1070, Click Chemistry Tools) in a 300 μl PBS ...
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bioRxiv - Bioengineering 2023Quote: ... and 2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl) acetic acid (BTTAA) (catalog no. 1236) were purchased from Click Chemistry Tools. Iodoacetamide and 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride (DMB ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... is incubated with DBCO-Peg5-NHS Ester (linker; 10mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (10 μg of antibody and 2.8 μg of linker ...
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bioRxiv - Biochemistry 2020Quote: ... is combined with DBCO-Peg5-NHS Ester (linker; 10 mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (50 mg of Rabbit IgG and 4.6 mg of linker) ...
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Versatile and Robust method for Antibody Conjugation to Nanoparticles with High Targeting EfficiencybioRxiv - Biochemistry 2021Quote: ... DBCO-PEG4-NHS-ester and N3-PEG4-NHS-ester were purchased from Click Chemistry Tools (United States). All the chemicals were used without further purifications.
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bioRxiv - Bioengineering 2020Quote: ... DBCO-NHS ester (1160, Click Chemistry Tools), poly(ethylene glycol ...
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bioRxiv - Cancer Biology 2022Quote: ... TCO-NHS ester (Click Chemistry Tools, USA), and p-SCN-Bn-deferoxamine (Macrocyclics ...
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bioRxiv - Bioengineering 2022Quote: ... DBCO-NHS ester (1160, Click Chemistry Tools), NHS-PEG-TCO ester (A137-10 ...
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bioRxiv - Biophysics 2024Quote: ... AFDye 488 NHS Ester (Click Chemistry Tools) was added to FUS at a dye:protein molar ratio of 20:1 and incubated in dark at room temperature for 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... and dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester (DBCO-Sulfo-NHS ester, Click Chemistry Tools, prepared as 10 mM stock in DMSO) at a 2500:1:1000 molar ratio of dye:particle:DBCO ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and TCO-PEG4-NHS ester (Click Chemistry Tools), respectively.
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bioRxiv - Cancer Biology 2022Quote: ... tetrazine-PEG5-NHS ester (Click Chemistry Tools, USA), TCO-NHS ester (Click Chemistry Tools ...
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bioRxiv - Bioengineering 2021Quote: ... Alkyne-PEG4-NHS Ester (Click Chemistry Tools > 95%), Bovine serum albumin alkyne (Click Chemistry Tools) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools) was first dissolved at 20mM in DMSO and added to the Fibrinogen solution dropwise to a 0.16mM final concentration ...
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bioRxiv - Synthetic Biology 2023Quote: ... Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools) was first dissolved at 8.8mM in DMSO and added to GelMA solution dropwise with stirring to 0.88mM final concentration ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
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bioRxiv - Immunology 2023Quote: ... Dibenzocyclooctyne-PEG4-N-hydroxysuccinimidyl ester (DBCO-PEG4-NHS ester) and Alexa Fluor 647 DBCO (AFDye 647 DBCO) were purchased from Click Chemistry Tools. Alexa Fluor 790 Succinimidyl Ester ...
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG-TCO ester (A137-10, Click Chemistry Tools), poly(ethylene glycol ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCB-NHS ester was purchased from Click Chemistry Tools or Merck/Sigma and stored in dry dimethylformamide (DMF ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody was modified with DBCO-Sulfo-NHS ester (Click Chemistry Tools, #A124) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Bioengineering 2021Quote: ... Cy5.5-NHS ester (purchased from Click Chemistry Tools, for Lys-cy5.5 conjugate 10), or DBCO–Cy5.5 (purchased from Click Chemistry Tools ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dibenzocyclooctyne (DBCO)-PEG4-NHS ester was purchased from Click Chemistry Tools (Scottsdale, AZ). Na125I was purchased from PerkinElmer (Waltham ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL antibodies were then modified with UV-cleavable azido-NHS ester (Click Chemistry Tools) at 1:15 molar ratio for 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were incubated with DBCO-PEG5-NHS Ester linker (Click Chemistry Tools, cat. no. A102P) at a 4:1 molar ratio linker:antibody for 2 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Ligands (GFP and mCherry) were modified using trans-Cyclooctene (TCO)- PEG4-NHS Ester (Click Chemistry Tools) to generate GFP-TCO and mCherry-TCO ...
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bioRxiv - Bioengineering 2024Quote: ... and were used for conjugation with dibenzocyclooctyne (DBCO)-N-hydroxysuccinimidyl (NHS) ester (#A133, Click Chemistry Tools). Typically ...
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bioRxiv - Cell Biology 2023Quote: ... alkyne-tagged fatty acid analogs (alkyne-myristate (13-tetradecylnoic acid; 13-TDYA; Click Chemistry Tools 1164) or alkyne-palmitate (15-hexadecynoic acid ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... BTX was maintained at ~500 μM when reacting with TCO linker (TCO-PEG4-NHS ester, Click Chemistry Tools). In the aforementioned protein-linker reactions ...
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bioRxiv - Bioengineering 2021Quote: ... Alkyne-PEG4-Acid (Click Chemistry Tools), Copper(II ...
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bioRxiv - Bioengineering 2022Quote: ... biotinylated DNAs at 200 μM were also adjusted to pH 8.0 and conjugated with UV cleavable DBCO-NHS ester (Click Chemistry Tools) at 1:20 molar ratio for 2 h ...
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bioRxiv - Bioengineering 2022Quote: ... 30 μL of 200 μM amine-ended biotinylated oligos was incubated with 100 mM photocleavable DBCO-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Bioengineering 2021Quote: ... while 30 μL of 200 μM cDNAs were incubated with 1.2 μL of 100 mM UV-cleavable DBCO-NHS ester (20 equivalents to cDNAs; Click Chemistry Tools) in 30% DMF for 4 h ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Bioengineering 2021Quote: Alexa Fluor 647-conjugated RBD was synthesized by the following methods: AFDye 647-NHS ester (Click Chemistry Tools, 1.8 mg, 1.85 µmol) was added to a solution of RBD protein (0.84 mg ...
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bioRxiv - Bioengineering 2022Quote: ... Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale, AZ). Cytochalasin D was obtained from Santa Cruz Biotech ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... sequences were modified with dibenzocyclooctyne (DBCO) for click chemistry reaction with antibody azides by incubation with dibenzocyclooctyne (DBCO)-sulfo-N-Hydroxysuccinimide (NHS)-ester (Click Chemistry Tools cat. no. A124) at a 1:1000 molar ratio for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: For the acid-cleavable reagent (DADPS Biotin Azide, CLICK CHEMISTRY TOOLS) ligated sample ...