Mouse Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit citations for LifeSpan BioScience

Choroid-resident macrophages maintain local vasculature and RPE integrity and spontaneously regenerate following depletion

Xiao Yang, et al., bioRxiv - Immunology 2019

Quote: ... Pigment epithelium-derived factor (PEDF) protein levels were measured in the samples using the Mouse SERPINF1/PEDF ELISA kit (LifeSpan BioSciences; LS-F36110). Protein levels of VEGF ...

Kupffer Cell Release of Platelet Activating Factor Drives Dose Limiting Toxicities of Nucleic Acid Nanocarriers

Meredith A. Jackson, et al., bioRxiv - Bioengineering 2020

Quote: ... Mouse PAF ELISA kits were purchased from LifeSpan Biosciences (Seattle, WA, USA). Plasma samples were assayed in duplicate according to kit manual using 50 µL samples in each well ...

Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signaling

Gang Chen, et al., bioRxiv - Neuroscience 2022

Quote: Mouse hevin ELISA kit was purchased from LifeSpan BioSciences (Catalog: LS-F12654). Human hevin ELISA kit was from Cloud-Clone Corp (Catalog ...

Two-photon microscopy in vivo reveals brain vessel type-specific loss of glycocalyx caused by apoM/S1P signaling impairment

Krzysztof Kucharz, et al., bioRxiv - Neuroscience 2022

Quote: ... The concentrations of blood plasma syndecan-1 were measured using a Mouse SDC1 ELISA Kit (#LS-F28946, LifeSpan BioSciences, Inc.) according to the manufacturer’s instructions ...

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases

Mahlon Collins, Yang Li, Robert Bowser, bioRxiv - Neuroscience 2019

Quote: ... mouse monoclonal anti-scaffold attachment factor B (SAFB, Lifespan Biosciences, Seattle, WA, USA, 1:100), mouse monoclonal anti-SAFB (Proteintech ...

Mechanism Underlying the Immune Responses of a Sublingual Vaccine for SARS-CoV-2 with RBD Antigen and Adjuvant, Poly(I:C) or AddaS03, in Non-human Primates

Tetsuro Yamamoto, et al., bioRxiv - Immunology 2023

Quote: ... were assayed using ELISA using the Monkey IL12 ELISA Kit (LifeSpan BioSciences, Inc.), Monkey IFN alpha (pan ...

Mechanism Underlying the Immune Responses of a Sublingual Vaccine for SARS-CoV-2 with RBD Antigen and Adjuvant, Poly(I:C) or AddaS03, in Non-human Primates

Tetsuro Yamamoto, et al., bioRxiv - Immunology 2023

Quote: ... Monkey IFN alpha (pan) ELISA Kit (LifeSpan BioSciences Inc), Monkey IFN-gamma ELISA Kit (U-CyTech biosciences) ...

Quercetin Suppresses TNBC Cell by Targeting ORM2

Zhijun Chen, bioRxiv - Cancer Biology 2023

Quote: ... CYCS/Cytochrome c ELISA Kit (LS-F11267) from LifeSpan BioSciences was used to determine the apoptosis of cells following the instructions of the kit ...

E-Cigarette Synthetic Cooling Agent WS-23 and Nicotine Aerosols Differentially Modulate Airway Epithelial Cell Responses

Marko Manevski, et al., bioRxiv - Pharmacology and Toxicology 2022

Quote: The protein levels of ICAM-1 and IL-6 were determined using human ELISA kits against ICAM-1 (LifeSpan Biosciences Inc., Seattle, WA) and IL-6 (BioLegend Inc. ...

Hypothalamic-pituitary-adrenal axis dysfunction worsens epilepsy outcomes and increases SUDEP risk

Trina Basu, et al., bioRxiv - Neuroscience 2022

Quote: ... or a Human CRH/CRH ELISA kit (Lifespan Biosciences, cat. #LS-F5352) and ran 5 uL aliquots of each sample in duplicate ...

Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells

Shuangfen Tao, et al., bioRxiv - Cancer Biology 2019

Quote: ... The ELISA for Bcl-2 was performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc, Seattle, WA, USA).

SPOP Promotes Ubiquitination and Degradation of MyD88 to Suppress the Innate Immune Response

Qinghe Li, et al., bioRxiv - Immunology 2019

Quote: ... Then the culture supernatants were collected and the levels of the indicated cytokines were determined using a chicken IL-1B/IL-1 beta ELISA kit (LifeSpan BioSciences)

Anopheles salivary apyrase regulates blood meal hemostasis and drives malaria parasite transmission

Zarna Rajeshkumar Pala, et al., bioRxiv - Microbiology 2023

Quote: ... ELISA (From LifeSpan BioSciences, Mouse Fibrin Degradation Product D-dimer ELISA Kit (LS Bio ...

HIV Infection impairs the Host Response to Mycobacterium tuberculosis Infection by altering Surfactant Protein D function in the Human Lung Alveolar Mucosa

Anwari Akhter, et al., bioRxiv - Cell Biology 2023

Quote: ... SP-A levels were measured by Human SP-A ELISA kit (LifeSpan Biosciences, Inc., Seattle, WA) and C3 levels by Human Complement 3 ELISA kit (Abcam ...

Intravenous sulforhodamine B reduces alveolar surface tension, improves oxygenation and reduces ventilation injury in a respiratory distress model

You Wu, Tam L. Ngyuen, Carrie E. Perlman, bioRxiv - Physiology 2020

Quote: ... and von Willbrand factor (vWF; Lifespan Biosciences). For all assays ...

Profiling the neuroimmune cascade in 3xTg mice exposed to successive mild traumatic brain injuries

Alyssa F. Pybus, et al., bioRxiv - Neuroscience 2023

Quote: ... and 0.1 μg/μL in 50ul for the CD68 and TMEM119 ELISA kits (Lifespan Biosciences LS-F11095 and LS-F52734). These protein concentrations were selected because they fell within the linear range of absorbance vs ...

Quantitative Analysis of Tissue Secretome Reveals the Diagnostic and Prognostic Value of Carbonic Anhydrase II in Hepatocellular Carcinoma

Xiaohua Xing, et al., bioRxiv - Cancer Biology 2020

Quote: The serum levels of CA2 in HCC patients were analyzed using an ELISA assay kit (cat No. LS-F29508, LifeSpan BioScience Inc., USA) following the manufacturer’s instructions ...

Single cell chromatin accessibility reveals pancreatic islet cell type- and state-specific regulatory programs of diabetes risk

Joshua Chiou, et al., bioRxiv - Genomics 2019

Quote: ... The following primary antibodies were used: mouse anti-NKX6-1 (LifeSpan BioSciences, 1:250), guinea pig anti-INS (Dako ...

Core spliceosomal Sm proteins as constituents of cytoplasmic mRNPs in plants

Malwina Hyjek-Składanowska, et al., bioRxiv - Cell Biology 2019

Quote: ... mouse anti-U2B’’ (LifeSpan Bioscience, Seattle, WA, USA; 1:20), rat anti-RNA pol II (Chromotek ...

Integrative Clinical and Molecular Characterization of Translocation Renal Cell Carcinoma

Ziad Bakouny, et al., bioRxiv - Cancer Biology 2021

Quote: ... the anti-LAG3 antibody (1/10,000, 17B4 mouse monoclonal antibody, LifeSpan Biosciences) was detected using the Opal 560 fluorophore (1:150 ...

Endothelial Lipase Variant, T111I, Does Not Alter Inhibition by Angiopoietin-like Proteins

Kelli L. Sylvers-Davie, et al., bioRxiv - Biochemistry 2023

Quote: ... Primary antibody dilutions were 1:3000 for a mouse monoclonal antibody against EL (LIPG antibody clone 3C7, Lifespan Biosciences), 1:2000 for a goat antibody against beta-actin (Abcam) ...

Adipose-tissue derived signals control bone remodelling

Maria-Bernadette Madel, et al., bioRxiv - Cell Biology 2020

Quote: ... alkaline phosphatase (ALP) and parathyroid hormone (PTH) were measured by ELISA assays according to the manufacturer’s protocol (LifeSpan Biosciences, Inc., Seattle, WA).

Nutrient Sensing Receptor GPRC6A Regulates mTORC1 Signaling and Tau Biology

Chao Ma, et al., bioRxiv - Neuroscience 2024

Quote: ... GPRC6A (mouse, LifeSpan BioSciences, #LS-B14489), AKT (Cell Signaling Tech ...

Interferon lambda signals in maternal tissues to exert protective and pathologic effects in a gestational-stage dependent manner

Rebecca L. Casazza, et al., bioRxiv - Immunology 2022

Quote: ... Viral foci were detected using 500 ng/mL of anti-flavivirus mouse monoclonal antibody E60 (66) or 1:1000 dilution of goat anti-RUBV antibody (Lifespan Biosciences LC-C103273/39321), 1:5000 dilution of an HRP conjugated goat anti-mouse IgG (Sigma #A8924 ...

ALPPL2 is a highly specific and targetable tumor cell surface antigen

Yang Su, et al., bioRxiv - Cancer Biology 2020

Quote: ... or anti-ALPPL2 mouse antibody (Clone SPM593, Lifespan Biosciences) were diluted 1:500 into 2% donkey serum/PBS and incubated with the tissue slides at 4°C overnight ...

Regulatory mechanisms in multiple vascular diseases locus LRP1 involve repression by SNAIL and extracellular matrix remodeling

Lu Liu, et al., bioRxiv - Molecular Biology 2023

Quote: ... mouse anti-TIMP3 (LS-B2576, Lifespan Biosciences, Seattle, Washington, USA), mouse anti-CYR61 (sc-374129 ...

A mechanism for the elimination of IgE plasma cells

Adam K. Wade-Vallance, et al., bioRxiv - Immunology 2022

Quote: ... Goat polyclonal F(ab’)2 anti-mouse Igκ (αIgκ; LifeSpan Biosciences), rat anti-mouse IgE clone R1E4 ...

FAM57B is a modulator of ceramide synthesis that regulates sphingolipid homeostasis and synaptic composition in the developing brain

Danielle L. Tomasello, et al., bioRxiv - Neuroscience 2021

Quote: ... Antibodies: 1:00 Synaptotagmin-1 (Lifespan Bioscience), GAD65 + GAD67 (Abcam) ...

Intravenous sulforhodamine B reduces alveolar surface tension, improves oxygenation and reduces ventilation injury in a respiratory distress model

You Wu, Tam L. Ngyuen, Carrie E. Perlman, bioRxiv - Physiology 2020

Quote: ... plasminogen activator inhibitor 1 (PAI-1; Lifespan Biosciences) and von Willbrand factor (vWF ...

FAM57B is a modulator of ceramide synthesis that regulates sphingolipid homeostasis and synaptic composition in the developing brain

Danielle L. Tomasello, et al., bioRxiv - Neuroscience 2021

Quote: ... Antibodies: 1:100 Synaptotagmin-1 (Syt1, Lifespan Bioscience), Vesicular Glutamate 1 and 2 (VGlut1/2 ...

Sulfatase 2 Inhibition Sensitizes Triple-Negative Breast Cancer Cells to Chemotherapy Through Augmentation of Extracellular ATP

Jasmine M Manouchehri, et al., bioRxiv - Cancer Biology 2023

Quote: ... The concentrations of sulfatase 1 and 2 (Lifespan Biosciences, Cat# LS-F66757-1 and LS-F35926-1) were measured in the supernatants of the examined cell lines at basal level via enzyme-linked immunoassay (ELISA ...

Single-molecule analysis of specificity and multivalency in binding of short linear substrate motifs to the APC/C

Nairi Hartooni, et al., bioRxiv - Biophysics 2021

Quote: ... Excess NeutrAvidin was washed away and incubated with either biotin-conjugated mouse monoclonal anti-Strep-Tag II antibody (LifeSpan BioSciences, Inc.) or biotin-conjugated rabbit polyclonal anti-GFP antibody diluted in buffer C + BSA (0.1 mg/ml Molecular Biology Grade Bovine Serum Albumin) ...

GATA2 regulates mast cell identity and responsiveness to antigenic stimulation by promoting chromatin remodeling at super-enhancers