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Citations for Bioo Scientific Corporation :
1 - 22 of 22 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were constructed from mRNA using KAPA mRNA HyperPrep Kit (KK8580) and NEXFlex barcoded adaptors (Bioo Scientific). High-throughput sequencing was performed using a HiSeq 2500 instrument (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA libraries were prepared using the NEXTflex Rapid Directional RNAseq Kit (BioO Scientific Corp.). Fragmentation of the mRNA was achieved by incubating 95°C for 13 minutes to produce ∼300 bp fragments ...
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bioRxiv - Cell Biology 2023Quote: ... polyadenylated mRNA was enriched using Nextflex Poly(A) Beads (NEXTflex, Bioo Scientific Corp, 512980) and cDNA libraries were prepared using a NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific Corp ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNAs were enriched from 10 μg total RNA with NEXTflex Poly(A) Beads (Bioo Scientific). These species were then fragmented ...
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bioRxiv - Molecular Biology 2019Quote: RNA-seq samples were prepared using the NEXTflex Rapid Directional mRNA-seq Kit (Bioo Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA enrichment was performed using NEXTflex™ Poly(A) Beads (BIOO Scientific Corporation, Texas, USA) before library preparation with the NEXTflex Rapid Directional qRNA-Seq Library Prep kit for Illumina sequencing (BIOO Scientific Corporation ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA enriched sequencing libraries were made with the NEXTflex Rapid Directional RNAseq Kit (BioO Scientific Corp.) and corresponding protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA cDNA libraries were prepared using NEXTflex rapid directional mRNA-seq kit (Bioo Scientific, NOVA-5138-10). Libraries were sequenced using the Illumina SBS platform.
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bioRxiv - Neuroscience 2020Quote: ... mRNA was purified from 2 μg of total RNA by NEXTflex poly(A) beads (Bioo Scientific, 512981), subjected to fragmentation and first and second strand syntheses ...
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bioRxiv - Molecular Biology 2019Quote: The RNA-seq libraries were prepared using the NEXTflexTM Rapid Directional mRNA-Seq Kit (NOVA-5138, Bioo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... mRNA was enriched from 1µg of total RNA using NEXTflex™ Poly(A) Beads (Bioo Scientific, NOVA-512979) and strand-specific directional libraries were generated using the NEXTflex™ Rapid Directional RNA-Seq Kit (Bioo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Following Poly-A selection mRNA libraries were prepared according to manufacturer’s instructions (Bioo Scientific Corp., NOVA-5138-08), except that the RNA was incubated at 95°C for 13 min to generate optimal fragment sizes ...
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bioRxiv - Developmental Biology 2021Quote: ... Following Poly-A selection mRNA libraries were generated according to manufactures instructions (Bioo Scientific Corp., NOVA-5138-08) except RNA was incubated for 13 min at 95°C to generate optimal fragment sizes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... total RNA was used to construct barcoded cDNA libraries using a NEXTflex™ Rapid Directional mRNA-seq kit (Bioo Scientific). Briefly ...
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bioRxiv - Genetics 2020Quote: ... In total 8 groups were prepared for RNA-seq libraries with 500 ng mRNA as starting materials using NEXTflex Illumina qRNA-Seq Library Prep Kit (Bioo Scientific). In short ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated and depleted mRNA samples were used for construction of Illumina-compatible single-end libraries using the NEXTflex Rapid Directional mRNA-Seq Bundle - Barcodes 1-24 (BIOO Scientific). Barcoded libraries were submitted to the Vermont Genetics Network (VGN ...
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bioRxiv - Molecular Biology 2023Quote: RNA-seq libraries for three biological replicates were generated with the NEXTflexTM Rapid Directional mRNA-Seq Bundle with poly(A) beads (NOVA-5138, Bioo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq libraries were prepared from purified 5EU-labeled RNA and from total RNA with the NEXTflex Rapid Directional mRNA-seq Kit (Bioo Scientific, 5138-10). Sequencing was performed on an Illumina HiSeq 2500 with a standard 40-cycle run ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mRNA was enriched from approximately 50 ng of high-quality total RNA with NEXTflex™ Poly(A) Beads (Bioo Scientific, Austin, TX, USA), and used to produce RNA-seq libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Fourteen µL of this mRNA-enriched poly(A)-tailed RNA was used as the input for the NEXTflex Rapid Directional qRNA-Seq kit (Bioo Scientific, #NOVA-5130-04). Library preparation was performed according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Poly-A tail selection was used to enrich for messenger RNA (mRNA) using the NEXTflex Poly(A) Beads kit (Bioo Scientific Corp, cat #512980). RNA-Seq library preparation was carried out using NEXTflex Rapid Directional qRNA-Seq kit (Bioo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The number of unique fragments (or read pairs) was determined for each transcript using the script provided by Bioo Scientific (dqRNASeq; settings: s=8, q=0, m=1). Counts that were used for further analysis were based on a unique combination of start and stop positions and barcodes (USS + STL) ...