Recombinant Human IL2RB citations for Cell Signaling Technology

APP binds to the EGFR ligands HB-EGF and EGF, acting synergistically with EGF to promote ERK signaling and neuritogenesis

Joana F. da Rocha, et al., bioRxiv - Neuroscience 2020

Quote: ... Human recombinant EGF was from Cell Signaling and was used at a 100 ng/mL working concentration (WC) ...

eIF6 rebinding dynamically couples ribosome maturation and translation

Pekka Jaako, et al., bioRxiv - Cell Biology 2021

Quote: ... recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 µg/mL ...

A syngeneic spontaneous zebrafish model of tp53-deficient, EGFR^viii, and PI3KCA^H1047R-driven glioblastoma reveals inhibitory roles for inflammation during tumor initiation and relapse in vivo

Alex Weiss, et al., bioRxiv - Cancer Biology 2023

Quote: ... or 0.05ng/nL human CSF-1 recombinant protein (Cell Signaling Technology, 8929SC).

Interferon-gamma Induces Melanogenesis Via Post-Translational Modification of Tyrosinase

Xuan Mo, et al., bioRxiv - Molecular Biology 2021

Quote: ... Human recombinant IFN-gamma (IFNG) was purchased from Cell Signaling Technology (catalog #8901). The concentration used was 100 ng/ml ...

The unjamming transition is distinct from the epithelial-to-mesenchymal transition

Jennifer A. Mitchel, et al., bioRxiv - Biophysics 2019

Quote: ... cells were treated with recombinant human TGF-β1 (10 ng/ml, Cell Signaling Technology) 59 ...

Anti-CoVid19 plasmid DNA vaccine induces a potent immune response in rodents by Pyro-drive Jet Injector intradermal inoculation

Tomoyuki Nishikawa, et al., bioRxiv - Immunology 2021

Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...

Preclinical study of DNA vaccines targeting SARS-CoV-2

Hiroki Hayashi, et al., bioRxiv - Immunology 2020

Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...

Interferon-Gamma Signaling Promotes Melanoma Progression and Metastasis

Bo Zhou, et al., bioRxiv - Cancer Biology 2021

Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...

Neuronal pentraxin Nptx2 regulates complement activity in the brain

Jiechao Zhou, et al., bioRxiv - Neuroscience 2022

Quote: ... or DIV12 neurons in the presence of recombinant protein (see recombinant protein below) or C1q-blocking antibody (Recombinant Anti-C1q antibody (ab182451)) / IgG (Normal Rabbit IgG,Cell Signaling Technology). For LPS induced activation of microglia ...

Spatio-temporal dynamics of nuclear CREB1: what does it mean?

Luz E. Farias Altamirano, et al., bioRxiv - Neuroscience 2022

Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...

Macrophages sense ECM mechanics and growth factor availability through cytoskeletal remodeling to regulate their tissue repair program

Matthew L. Meizlish, et al., bioRxiv - Immunology 2023

Quote: ... and recombinant murine MCSF (Cell Signaling Technologies, 5228) at 100 ng/mL for 2D culture and 200 ng/mL for 3D culture ...

Endothelial CXCL12 regulates neovascularization during tissue repair and tumor progression

Zeshaan N. Maan, et al., bioRxiv - Molecular Biology 2020

Quote: ... 100 ng/mL recombinant CXCL12 + U0126 (Cell Signaling Technology), or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology) ...

Roles of C/EBPα, GATA2, TGF-β-signaling, and epigenetic regulation in the expression of basophil-specific protease genes

Ryotaro Tojima, et al., bioRxiv - Immunology 2024

Quote: ... and recombinant mouse TGF-β (5231, Cell Signaling Technology) were purchased from the indicated sources.

Endothelial CXCL12 regulates neovascularization during tissue repair and tumor progression

Zeshaan N. Maan, et al., bioRxiv - Molecular Biology 2020

Quote: ... or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology). After 72 hours ...

TNF controls a speed-accuracy tradeoff in the apoptotic decision to restrict viral spread

Jennifer Oyler-Yaniv, et al., bioRxiv - Systems Biology 2020

Quote: ... with dose titrations of recombinant mouse TNFα (Cell Signaling Technologies), 30μM Z-VAD(OMe)-FMK (Cayman Chemical) ...

Early acquisition of S-specific Tfh clonotypes after SARS-CoV-2 vaccination is associated with the longevity of anti-S antibodies

Xiuyuan Lu, et al., bioRxiv - Immunology 2023

Quote: ... recombinant ancestral S protein (S1+S2, Cell Signaling Technology; 1 µg/ml) or recombinant nucleocapsid protein (Acrobiosystems ...

Interferon-gamma Induces Melanogenesis Via Post-Translational Modification of Tyrosinase

Xuan Mo, et al., bioRxiv - Molecular Biology 2021

Quote: Mouse recombinant IFN-gamma (Ifng) was purchased from Cell Signaling Technology (catalog #5222). The typical concentration used was 10 ng/ml ...

LPA/PKD-1 signaling promotes development of arteriolar niche that supports self-renewal of breast cancer stem cells and stemness

Yinan Jiang, et al., bioRxiv - Cancer Biology 2020

Quote: ... mouse anti-human/rat CD44 (5640S) and rabbit anti-human/mouse ALDH1A1 (12035S) (Cell Signaling Technology), rabbit anti-human/mouse CD36 (ab133625 ...

Loss of TIM4-Dependent Efferocytosis in Kupffer Cells Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis

Hongxue Shi, et al., bioRxiv - Pathology 2024

Quote: ... human liver sections were incubated at 4 °C overnight with anti-human TIM4 (Cell signaling technology, #75484T ...

Multi-organ signaling mobilizes tumor-associated erythroid cells expressing immune checkpoint molecules

Yasuyo Sano, et al., bioRxiv - Cancer Biology 2020

Quote: ... human PDL1 (E1L3N; Cell Signaling Technology), and EF5 (ELK3-51 ...

A mosaic renal myeloid subtype with T-cell inhibitory and protumoral features is linked to immune escape and survival in clear cell renal cell cancer

Dorothee Brech, et al., bioRxiv - Immunology 2020

Quote: ... rabbit-anti-human FOXO1 (Cell Signaling) and mouse-anti-human CD3 (UCHT1 ...

Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins

Takeshi Uemura, et al., bioRxiv - Neuroscience 2020

Quote: ... or human IGF-I (Cell Signaling) was applied to the cultured CGCs at DIV2 ...

Antibody-induced glomerulonephritis is amplified by RTEC-intrinsic IL-17 signaling and restrained by IL-17-mediated induction of the endoribonuclease Regnase-1 (Zc3h12a)

De-Dong Li, et al., bioRxiv - Immunology 2021

Quote: ... human anti-Iκbζ (9244, Cell signaling), human/mouse anti-YY1 (sc-7341 ...

CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites

Michi Miura, Honglin Chen, bioRxiv - Molecular Biology 2020

Quote: ... Cells were then incubated with the recombinant pAG-MNase (Cell Signaling, 40366; 1:33 volume) in 150 µl Perm buffer per well for an hour at room temperature ...

Enhanced feature matching in single-cell proteomics characterizes response to IFN-γ and reveals co-existence of different cell states

Karl K. Krull, Syed A. Ali, Jeroen Krijgsveld, bioRxiv - Systems Biology 2024

Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...

pHLARE: A Genetically Encoded Ratiometric Lysosome pH Biosensor

Bradley A. Webb, et al., bioRxiv - Cell Biology 2020

Quote: ... and human LAMP1 (Cell Signaling Technologies; 9091) diluted in blocking buffer ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-mouse/human STING (Cell Signaling #13647), anti-human phospho-IRF3 (Abcam ab196035) ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-human phospho-STING (Cell Signaling #19781), anti-mouse phospho-STING (Cell Signaling #72971) ...

ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

Quote: ... anti-mouse/human Actin (Cell Signaling #3700), anti-human phospho-STING (Cell Signaling #19781) ...

S1P-S1PR1 activity controls VEGF-A signaling during lymphatic vessel development

AM Golding-Ochsenbein, et al., bioRxiv - Developmental Biology 2019

Quote: ... rabbit anti-human Rab5 (Cell Signaling, #3547P). After an intense wash step in 0.1% Triton-X PBS ...

Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of Post-Transcriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis

Katherine E. Uyhazi, et al., bioRxiv - Developmental Biology 2019

Quote: ... CDK4 monoclonal mouse anti-human (Cell Signaling), Cyclin D1 monoclonal mouse anti-human (Cell Signaling) ...

Nuclear soluble cGAS senses DNA virus infection

Yakun Wu, et al., bioRxiv - Immunology 2021

Quote: ... anti-human cGAS (Cell Signaling Technology, #79978), anti-mouse cGAS (Cell Signaling Technology ...

Splice switching oligonucleotide mediated gene knockdown in B cells and plasma cells

Anne Marchalot, et al., bioRxiv - Immunology 2020

Quote: ... rabbit anti-human RelA antibody (Cell Signaling) or mouse anti-beta-actin antibody (Sigma) ...

Bone morphogenetic protein 4 reduces global H3K4me3 to inhibit proliferation and promote differentiation of human neural stem cells

Sonali Nayak, et al., bioRxiv - Neuroscience 2020

Quote: ... Human OCT4 (Cell Signaling Technology, Cat#4641) and CCND1 (Cell Signaling Technology ...

Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

Thomas Chapman, et al., bioRxiv - Immunology 2019

Quote: ... rabbit anti-human p38 #9212 (Cell Signaling), rabbit anti-human LAMTOR1 #8975 D11H6 (Cell Signaling) ...

A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913