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Citations for Cell Signaling Technology :
1 - 50 of 308 citations for Recombinant Human CD28 Molecule Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-human CD28 (clone D2Z4E, Cell Signaling) followed by anti-rabbit IgG Pierce™ protein A/G magnetic beads (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Human recombinant EGF was from Cell Signaling and was used at a 100 ng/mL working concentration (WC) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 µg/mL ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... CD28 (Cell Signaling, 38774), ICOS (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... or 0.05ng/nL human CSF-1 recombinant protein (Cell Signaling Technology, 8929SC).
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bioRxiv - Molecular Biology 2021Quote: ... Human recombinant IFN-gamma (IFNG) was purchased from Cell Signaling Technology (catalog #8901). The concentration used was 100 ng/ml ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with recombinant human TGF-β1 (10 ng/ml, Cell Signaling Technology) 59 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-CD28 (clone D2Z4E, Cell Signaling), HRP-conjugated anti-mouse IgG (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... anti-CD28 pY191 (Cell Signaling Technology, catalog #16399), anti-AKT pT308 (Cell Signaling Technology cat# 2965) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-CD28 (1:1000, 38774, Cell Signaling), rabbit anti-GMAP210 (Gift from Michel Bornens) ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-tagged ALI1 and 6xHis-tagged control protein were incubated with streptavidin beads (Sepharose® Bead Conjugate, Cell Signaling Technology) that were already loaded with M4 (oxo-C14-HSL tagged with biotin ...
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bioRxiv - Neuroscience 2022Quote: ... or DIV12 neurons in the presence of recombinant protein (see recombinant protein below) or C1q-blocking antibody (Recombinant Anti-C1q antibody (ab182451)) / IgG (Normal Rabbit IgG,Cell Signaling Technology). For LPS induced activation of microglia ...
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bioRxiv - Cell Biology 2023Quote: ... and HRP-tagged goat anti-rabbit (Cell Signaling) antibodies.
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...
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bioRxiv - Bioengineering 2021Quote: ... and nuclear fractions to HRP-tagged Histone H3 (#12648, Cell Signaling). All blots were developed using Immobilon Luminata Forte (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were eluted in 1x kinase buffer (Cell Signaling), containing 150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... and CD19 CAR using PE-tagged protein L (58036S; Cell Signaling). B7-H3 CAR expression was determined using CD34-APC (FAB7227R ...
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bioRxiv - Microbiology 2023Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Bioengineering 2021Quote: ... and subsequently an HRP-tagged anti-Rabbit secondary antibody (#7074S, Cell Signaling). Whole cell lysates were normalized to HRP-tagged Actin antibody (#A3854 ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-rabbit and anti-mouse secondary antibodies (Cell Signaling Technology); Actin ...
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bioRxiv - Immunology 2023Quote: ... and recombinant murine MCSF (Cell Signaling Technologies, 5228) at 100 ng/mL for 2D culture and 200 ng/mL for 3D culture ...
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bioRxiv - Cell Biology 2019Quote: The following small molecule inhibitors were used in this study: MEK inhibitors: 2 μM PD 184352 (Cell Signaling 12147) and 10 μM Selumetinib (Selleckchem S1008) ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged proteins were immunoprecipitated using mouse anti-myc monoclonal antibody (Cell signaling) and rProtein A agarose (Genesee) ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng/mL recombinant CXCL12 + U0126 (Cell Signaling Technology), or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology) ...
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bioRxiv - Immunology 2024Quote: ... and recombinant mouse TGF-β (5231, Cell Signaling Technology) were purchased from the indicated sources.
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bioRxiv - Pathology 2020Quote: ... Horseradish peroxidase-tagged secondary anti-rabbit IgG was from Cell Signaling (#7074, 1:2000).
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bioRxiv - Molecular Biology 2020Quote: ... or 100 ng/mL recombinant CXCL12 + LY294002 (Cell Signaling Technology). After 72 hours ...
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bioRxiv - Systems Biology 2020Quote: ... with dose titrations of recombinant mouse TNFα (Cell Signaling Technologies), 30μM Z-VAD(OMe)-FMK (Cayman Chemical) ...
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bioRxiv - Physiology 2022Quote: ... followed by 1:10000 HRP-tagged anti-rabbit secondary antibody (Cell Signaling Technology, cat #7074). HRP signal was detected with chemiluminescent substrate (Pierce SuperSignal West Pico or Femto Plus ...
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat. 901514; rabbit, Cell Signaling 3724S), and two PLA probes (anti-rabbit-PLUS ...
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bioRxiv - Immunology 2021Quote: ... CD8+ and CD4+ T effectors were stimulated by addition to tissue culture flasks coated with anti-CD3 (produced in-house, clone OKT3) and anti-CD28 ([D2Z4E], Cell Signaling) antibodies or with anti-CD3/anti-CD28 Dynabeads (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... GST tagged PTP-PEST was pulled down via immunoprecipitation using GST antibody (Cell Signaling Technology, USA). Immunoprecipitated proteins were loaded on to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... Myc-tagged proteins were detected using myc-tag (9B11) mouse monoclonal antibody (Cell Signaling Technology #2276S) at 1:5000 dilution ...
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bioRxiv - Immunology 2023Quote: ... recombinant ancestral S protein (S1+S2, Cell Signaling Technology; 1 µg/ml) or recombinant nucleocapsid protein (Acrobiosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-ionized calcium-binding adaptor molecule 1 (Iba-1, FUJIFILM Wako Chemicals USA, Richmond, VA) and anti-glial fibrillary acidic protein (GFAP, Cell Signaling Technology, Danvers, MA) antibodies were diluted to 1:1,000 and used to incubate tissue sections overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... and Myc-tagged Rrp4 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Genetics 2022Quote: ... Myc-tagged Rrp4 proteins were detected with mouse anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP ...
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bioRxiv - Genetics 2022Quote: ... and Myc-tagged Rrp4 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Molecular Biology 2021Quote: Mouse recombinant IFN-gamma (Ifng) was purchased from Cell Signaling Technology (catalog #5222). The typical concentration used was 10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated with goat anti-mouse or goat anti-rabbit antibody tagged with horseradish peroxidase (Cell Signaling) and immunoreactive bands were detected by enhanced chemiluminesence (GE Healthcare and Boston Bioproducts) ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... Myc-tagged mCherry-PIF5 was detected using rabbit anti-Myc polyclonal antibodies (2272, Cell Signaling Technology, Danvers, MA) as the primary antibody at 1:100 dilution and donkey anti-rabbit AlexaFluor 555 antibodies (A31572 ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human/rat CD44 (5640S) and rabbit anti-human/mouse ALDH1A1 (12035S) (Cell Signaling Technology), rabbit anti-human/mouse CD36 (ab133625 ...
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bioRxiv - Pathology 2024Quote: ... human liver sections were incubated at 4 °C overnight with anti-human TIM4 (Cell signaling technology, #75484T ...
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bioRxiv - Cancer Biology 2020Quote: ... human PDL1 (E1L3N; Cell Signaling Technology), and EF5 (ELK3-51 ...
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bioRxiv - Immunology 2020Quote: ... rabbit-anti-human FOXO1 (Cell Signaling) and mouse-anti-human CD3 (UCHT1 ...