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Citations for Cell Signaling Technology :
1 - 50 of 71 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: We performed ChIP-qPCR using SimpleChIP® Universal qPCR Master Mix (Cell Signaling Technology, Danvers, MA), primers designed for the promoter region (0 – 1.5 kb upstream ...
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bioRxiv - Microbiology 2023Quote: Antibodies used for ChIP-qPCR analysis were: RPA (Cell Signaling, 2208S).
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bioRxiv - Microbiology 2020Quote: ... For MEK1/2 inhibition qPCR experiments MEFs were incubated with 10μM U0126 (Cell Signaling) or 10μM DMSO (vehicle ...
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bioRxiv - Cell Biology 2019Quote: ... and thereafter incubated for 1 hour at RT with primary antibodies (α-tubulin, Cell Signaling 1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 30min at room temperature (RT) followed by primary antibody (γ-H2A.X, Cell Signaling, mAb #9718) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were blocked for 60 min at RT in TBS-T + 5% nonfat milk (Cell Signaling) and incubated overnight in the same solution with the respective primary antibodies at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Blots were blocked for 60 min at RT in TBS-T + 5% nonfat milk (Cell Signaling) and incubated with the respective primary antibody at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS at RT for 1□h and incubated with primary antibodies (PGR - Cell signaling Technology 8757S ...
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated overnight at RT with a rabbit anti-HA primary antibody (Cell Signaling Technologies, C29F4). Following primary incubation ...
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bioRxiv - Biophysics 2020Quote: ... and then for 1 hr at RT with anti-rabbit IgG-HRP (Cell Signaling Technology Cat# 7074S) or anti-mouse IgG-HRP (Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Microbiology 2023Quote: ... or by incubation with HRP-conjugated horse anti-mouse IgG (Cell Signaling, 1:1000, 60 min, RT), wash (TBST × 3) ...
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bioRxiv - Molecular Biology 2019Quote: ... All samples were then processed for ChIP-qPCR using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technologies) as per manufacturer’s recommended protocol ...
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bioRxiv - Biophysics 2023Quote: ... while shaking) and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat ...
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bioRxiv - Immunology 2023Quote: ... incubated 2h on RT or overnight at 4°C with antibodies for Akt (1:1000, Cell signaling, #9272) or P-Akt (Phospho-Akt (Ser473 ...
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bioRxiv - Cell Biology 2023Quote: Chromatin samples for ChIP‒qPCR were prepared using a SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, 9003) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with the secondary antibody and DAPI for 1 h at RT and mounted with Prolong Gold (Cell Signaling). Images were obtained using a SP8 confocal microscope (Leica microsystems ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... blocked for 1 h at RT in incubated overnight in primary antibodies against RPS6Ser235/236 (Cell Signaling Technology, #4858S), P-RPS6Ser240/244 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... for 1 h at RT and mounted with Prolong™ Gold Antifade reagent (Cell Signaling Technology, Danvers, MA, USA). For whole- mount staining ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 1 hour at RT with primary antibody for the VEE marker APPL1 (Cell Signaling Technology), before 3x PBS Ca2+ washes and incubated for 1 hour at RT with anti-mouse 488 ± anti-rabbit 555 secondary antibodies (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
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bioRxiv - Developmental Biology 2022Quote: ... The following secondary antibodies were incubated for 1 hour at RT: anti-mouse IgG HRP-linked (7076; Cell Signaling Technology) and anti-rabbit IgG HRP-linked (7074 ...
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bioRxiv - Microbiology 2020Quote: ... Sections were permeabilized with 0.5 % Triton-X for 30 min at RT and stained over night with primary antibodies (rabbit anti-IFITM1 Cell Signaling 13126 S ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 h at RT in Odessy blocking buffer (Licor) and incubated overnight at 4°C with the following antibodies: IRF3 (Cell Signaling) 1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... Membranes were then incubated for one hour at RT in either appropriate (mouse or rabbit) Horse radish peroxidase (HRP) - linked secondary antibodies (Cell Signaling) in TBST for chemiluminescent detection ...
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bioRxiv - Microbiology 2020Quote: ... skin milk powder in PBST with 0.1% Tween 20) for 1 hour at RT and probed overnight with primary antibody (anti-HA, mAb #2367, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were incubated for 1 h at RT and detected using Mouse-anti-Myc antibody (1:1000, 9B11 clone, Cell Signaling). US28 was visualized with the rabbit-anti-US28 antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were then incubated for 60-180 min at RT with HA antibody (rabbit, 1:250, Cell signaling Technology, cat #C29F4) in PBS–GS blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS at RT for 1□h and incubated with primary antibodies (PGR - Cell signaling Technology 8757S; ESR1 - Cell signaling Technology 8644S ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed 3X in PBS and blocked for 1 h at RT with 5% BSA solution containing 5% normal goat serum (Cell Signaling) and 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in blocking buffer and applied for 1 hour at RT: rabbit anti-p53 (Cell Signaling Technology, 2527) at 1:200 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 min at RT and incubated in Alexa Fluor 488 Phalloidin (Cell Signaling Technology) at 1:1000 for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ChIP-qPCR and ChIP-seq samples were prepared following the instructions of the SampleChIPR Enzymatic Chromatin IP Kit (Cell Signaling, #9005). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% Tween 20) for 1 h at RT and then incubated with specific antibodies (eIF2α, P-eIF2α (Cat# 9722, 9721S, Cell Signaling™), HA (Cat#901501 ...
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bioRxiv - Biochemistry 2019Quote: ... in tris-buffered saline Tween 20 (TBS-T) for 1 h at RT and then blotted for phosphorylated proteins (Cell Signaling Technology; phospho-EGFR Y1068 #3777 ...
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bioRxiv - Cell Biology 2019Quote: ... 5% BSA (AmericanBio) in TBST (TBS with Tween 20) for 1 h at RT and incubated with AKT (1:1000, rabbit, Cell Signaling 9272), pAKT (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... The vibraslices were then incubated for overnight at RT with rabbit anti-HA antibody (1:1000; Cell Signaling Technology Cat# 3724, RRID:AB_1549585), followed by 1 hr with the Cy5-conjugated goat anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...