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Citations for Cell Signaling Technology :
1 - 50 of 1180 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA protein assay kit from Cell Signaling Technology. Thirty micrograms of total protein were mixed in a reducing sample buffer and used for mitochondrial apoptosis assay per the kit instruction ...
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bioRxiv - Immunology 2023Quote: The protein concentration was adjusted to 30 μg by adding protein lysis buffer (Cell Signaling Technology, Danvers, MA) and 4x loading buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples of equal protein concentration were prepared in reducing and denaturing conditions (Cat. No. 7722, Cell Signaling Technology).
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: GABARAP/Atg8a (1:1,000, E1J4E, Cell Signaling), Arl8 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Primary antibodies/concentrations used were as follows: rabbit anti-phospho-S6 ribosomal protein (Serine240/244) (1:100, Cell Signaling Technologies, #2215), rabbit anti-phospho-S6 ribosomal protein (Serine244/247 ...
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bioRxiv - Molecular Biology 2021Quote: ... Thirty μL magnetic protein G beads (SimpleChIP Kit #9003, Cell Signaling Technology) were added to the samples and incubated at 4°C with end-over-end rotation for 4 h ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... Abcam Cat# ab270521).The following antibody and the concentration used against human proteins were employed: αEGFR (1:1000 for western blot; Cell Signaling Technology Cat# 4267, RRID:AB_2246311), αEGFRL858R mutant specific (1:100 microscopy ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular cAMP concentrations were determined using a cyclic AMP XP Assay Kit (Cell Signaling Technology, catalog number 4339S, Danvers, MA) using 50μg total protein per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... STAT3 (Cell Signaling #30835, concentration 1:1000), pSTAT3-Ty705 (Cell Signaling #9131 ...
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bioRxiv - Cancer Biology 2020Quote: ... RelA/p65 (Cell Signaling #3034, concentration 1:1000), pRelA/p65-S536 (Cell Signaling #3033T ...
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bioRxiv - Cancer Biology 2020Quote: ... p53-S15 (Cell Signaling, #9284 concentration 1:1000), total AKT (Upstate #14276 ...
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bioRxiv - Cancer Biology 2020Quote: ... pSTAT3-Ty705 (Cell Signaling #9131, concentration 1:1000), p53-S15 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... AKT-S473 (Cell Signaling #9271S, concentration 1:1000) and anti-human IL-10 (R&D #MAB217-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... RelA/p65 (Cell Signaling #3034, concentration 1:1000), pRelA/p65-S536 (Cell Signaling #3033T ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of antibodies from Cell Signaling Technology is often not provided and thus was not listed here.
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bioRxiv - Cancer Biology 2020Quote: ... pRelA/p65-S536 (Cell Signaling #3033T, concentration 1:1000), STAT3 (Cell Signaling #30835 ...
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bioRxiv - Cancer Biology 2023Quote: ... pRelA/p65-S536 (Cell Signaling #3033T, concentration 1:1000), total AKT (Cell Signalling #2920 ...
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bioRxiv - Physiology 2019Quote: ... Immunocaptured proteins were isolated using Protein-G agarose beads (Cell Signaling Technology), washed multiple times with CHAPS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysis buffer (Cell Signaling) containing a protease inhibitor cocktail was added and the mixture homogenized ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody concentrations: anti-HA (rabbit, 1:1000, Cell Signaling, #3724), anti-β-actin (mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... or of 35 mg (for mouse tumors) total proteins using the PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit (Cell Signaling Technology), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... we used either HRP-conjugated protein A or HRP-conjugated protein G (both from Cell Signaling) because of high background from the immunoglobulin bands when we used secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibody concentrations are as follows: pAkt (Thr308) (Cell Signaling Cat. # D25E6) (anti-rabbit ...
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bioRxiv - Microbiology 2020Quote: ... S6 Ribosomal protein (5G10, Cell Signaling), BIM (C34C5 ...
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bioRxiv - Immunology 2019Quote: ... Protein A agarose beads (Cell signaling) were used for IgG pulldown ...
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bioRxiv - Physiology 2022Quote: ... and Biotinylated Protein ladder (Cell Signaling) were included on the gels ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysis buffer (Cell Signaling Technology) containing a protease inhibitor cocktail was added ...
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bioRxiv - Cell Biology 2023Quote: ... one for each protein (Cell Signaling). Each membrane piece was then incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with an equal molar concentration of anti-His antibody (Cell Signaling), followed by incubation with a secondary antibody (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies were used at the following concentration: anti-CD9 (1:500; Cell Signaling), anti-CD63 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies and concentrations were as follow: rabbit monoclonal anti-MSK1 (C27B2; Cell Signaling) 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were then collected by scraping and the CNMCS Compartmental Protein Extraction Kit (BioChain Cat# K3013010 Hayward, CA) with protease/phosphatase inhibitors (PI, Cell Signaling, Cat# 5872S, Danvers, MA) was used and the nuclear fractions were isolated for each treatment condition ...
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bioRxiv - Immunology 2019Quote: ... Quantified protein (between 200-500 µg) was incubated with protein-specific or isotype control antibody (Cell Signaling Technology) in lysis buffer at 4°C overnight with head over tail rotation ...
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bioRxiv - Immunology 2020Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology). Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins bound to Protein-A Dynabeads were pulled down using a magnetic separation rack (Cell Signaling, no. 14654). After 5 washes in NP-40 lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: Protein A magnetic beads (Cell Signaling Technology) were pre-washed with cell lysis buffer twice ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... S6 ribosomal protein (2217; Cell Signaling Technology), Akt1 (2938 ...
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bioRxiv - Immunology 2021Quote: ... coupled to Protein G beads (Cell Signaling) overnight with rotation at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... containing a Protein A slurry (Cell Signaling). A non-specific antiserum (control IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... protein A/G agarose beads (Cell Signaling) were added and allowed to rotate for another 2h ...
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bioRxiv - Molecular Biology 2021Quote: ... with added protease and phosphatase inhibitors at a 1:100 concentration (Cell Signaling Technology). Protein concentration was determined using a commercial colourimetric assay (Bio-Rad Protein Assay kit II ...