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Citations for Cell Signaling Technology :
1 - 50 of 2148 citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and anti-4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling #4083). The primary microglial yield was approximately 5 × 105 cells/flask with minimal astrocyte contamination ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; Cell Signaling Technology, USA), and finally samples were mounted with ProLong® Gold Antifade Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Following nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA), larvae were mounted in 80% glycerol between two coverslips and imaged using fluorescence confocal microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... Hydroxy-HIF-1α (Pro564) (#3434, Cell Signaling Technologies), VHL (#68547 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... anti-CD11a (clone IBL-6/2; Cell Signaling Techologies), Alexa Fluor 647-anti-Rat IgG (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Cell Signaling; Catalog No. 8961S). All images were captured on a Zeiss Apotome 2 microscope by using 20x ...
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bioRxiv - Microbiology 2021Quote: ... 6 diamidino-2-phenylindole (DAPI, 0.5 μg/mL in PBS, Cell Signaling Technology). Stained sections were mounted with ProLong® Gold Antifade Reagent (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were fixed with 4% paraformaldehyde for 5 minutes and blocked in 5% goat normal serum (Cell Signaling Technologies, 5425) for an hour before antibody detection using antibodies listed in Supplementary Tables S5-S6 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2019Quote: ... A fluorescent labelled secondary antibody conjugated with Alexa Fluor 647 as wells as Alexa Fluor 488 N-Hydroxysuccinimid (NHS, 1-Hydroxy-2,5-pyrrolidindion) and Hoechst-33342 staining solution was purchased from Cell signaling/Life Technologies (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D113-#4) using an anti-H3K27me3 antibody (Cell Signaling Technology, Ref: 9733-C36B11). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mg/mL) or 4 μL isotype control (2.5 mg/mL, Cell Signaling Technology) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
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bioRxiv - Developmental Biology 2019Quote: ... stained in 1mg/ml 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, Cell Signaling) pH of 5.9-6.1 overnight at 37C ...
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bioRxiv - Genetics 2019Quote: ... fixed with 4% paraformaldehyde for 30 minutes and then blocked with 5% normal goat serum (Cell Signaling) and 0.3% Triton X-100 for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... Membranes were incubated overnight at 4°C (5% BSA/PBS) with antibodies against total p21 (12D1, Cell Signaling), tubulin (Abcam) ...
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bioRxiv - Molecular Biology 2022Quote: ... α-tubulin (1:1000, incubated overnight in 5% BSA in 1 X PBST at 4°C, Cell Signaling), HA-HRP (1:6000 ...
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bioRxiv - Developmental Biology 2022Quote: ... for 2 h and stained overnight at 4 °C with anti-HA (Cell Signaling Technology, Cat# 3724S), anti-INO80B (Novus ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Abcam) at 4°C and for 2 hours at room temperature in HRP-conjugated secondary antibodies (Cell Signaling).
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 μm cryosections were incubated overnight at 4°C with an antiphosphoSmad1/5/8 antibody (1:500; Cell Signaling 9511S) in PBS with 2% horse serum and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C in 5% BSA/TTBS containing rabbit anti-STING (0.058 µg/ml, Cat. # 13647, RRID: AB_2732796; Cell Signaling Technology), anti-phospho-TMEM173/STING S366 (1 µg/ml ...
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bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated overnight at 4 °C with primary antibodies against phospho-ERK1/2 (1:1,000; Cell Signaling), ERK1/2 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... ATF-6 (Cell Signaling Technology - 65880), Beclin-1 (Cell Signaling Technology - 3495) ...
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bioRxiv - Cell Biology 2020Quote: ... GATA-6 (clone: D61E4; Cell Signaling); OCT4 ...
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bioRxiv - Cell Biology 2023Quote: ... α6 integrin (Cell Signaling Technology, 3750), β1 integrin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated overnight at 4°C with primary antibodies diluted in 5% goat serum/ PBS: rabbit anti-PAX6 (1:250, 60433S, Cell Signaling), rabbit anti-SOX1 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
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bioRxiv - Neuroscience 2023Quote: ... immunoblots were probed for anti-rabbit acetylated histone 3 at lysine 9 and histone 4 at lysine 5 (ac-H3K9 and ac-H4K5 respectively, Cell Signaling), anti-rabbit LC3B (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref: 901503; anti-H3K4me3 Cell Signaling, ref ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4°C with the rabbit anti-phospho-p44/42 MAPK (for pErk1/2) (Cell Signaling Technology) at 1:250 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyconal (3672, Lot 6, Cell Signaling); pMLC2 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyconal (6671, Lot 6, Cell Signaling) and mouse monoclonal (3675 ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were washed 3X for 5 minutes in 1X TBST and incubated overnight at 4°C in 5% BSA w/v in 1X TBST with primary antibodies raised against phospho-S6 (Cell Signaling 4858), S6 (Cell Signaling 2217) ...
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bioRxiv - Genetics 2021Quote: ... The following primary antibodies were used at 1:1000 dilution in PBST (0.05% Tween-20) and 5% non-fat dry milk and incubated overnight at 4°C: Myc (Cell Signaling Technology #2276), RBPJ (Cell Signaling Technology #5313) ...