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Citations for Cell Signaling Technology :
1 - 50 of 10000+ citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling #4083). The primary microglial yield was approximately 5 × 105 cells/flask with minimal astrocyte contamination ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; Cell Signaling Technology, USA), and finally samples were mounted with ProLong® Gold Antifade Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Following nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA), larvae were mounted in 80% glycerol between two coverslips and imaged using fluorescence confocal microscopy ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was carried out by using 4’,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Four different sections with no tissue loss were counted for the quantification of cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclear staining was carried out by using 4′,6-diamidino-2-phenylindole (DAPI; 4083S, Cell Signaling Technology, MA, USA). Larvae were imaged using fluorescence confocal microscopy.
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... anti-CD11a (clone IBL-6/2; Cell Signaling Techologies), Alexa Fluor 647-anti-Rat IgG (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... α-tubulin (1:1000, incubated overnight in 5% BSA in 1 X PBST at 4°C, Cell Signaling), HA-HRP (1:6000 ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Cell Signaling; Catalog No. 8961S). All images were captured on a Zeiss Apotome 2 microscope by using 20x ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 5% BSA in TBST: pMEK1/2 (Cell Signaling Technology, Danvers, MA, USA, 41G9, 1:1000), pERK1/2 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% BSA in TBST buffer for 2 h at RT and incubated overnight at 4°C with primary antibodies against ERK1/2 (1:1000; 9102s, Cell Signaling Technology), pERK1/2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... cl-caspase-6 (1:100, Cell Signaling 9761S), GFAP (1:2,000 AVES GFAP) ...
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bioRxiv - Microbiology 2021Quote: ... 6 diamidino-2-phenylindole (DAPI, 0.5 μg/mL in PBS, Cell Signaling Technology). Stained sections were mounted with ProLong® Gold Antifade Reagent (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... the lysates were incubated at 4°C for 5 h with antibodies for BRD4 (Cell Signaling Technology; 1:100). Antibody/antigen complexes were recovered using Dynabeads Protein A/G (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated overnight at 4 °C with primary antibodies against phospho-ERK1/2 (1:1,000; Cell Signaling), ERK1/2 (1:400 ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 μm cryosections were incubated overnight at 4°C with an antiphosphoSmad1/5/8 antibody (1:500; Cell Signaling 9511S) in PBS with 2% horse serum and 0.1% Triton X-100 ...
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bioRxiv - Physiology 2023Quote: ... blocked with 5% goat serum and incubated overnight at 4 °C with anti-TMEM16A (Cell Signaling, Rabbit D1M9Q 1:100) and CD31 (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... anti-pro/caspase-6 (Cell Signaling 9762 1:1000), anti-pro/caspase-7 (Cell Signaling 9492 ...
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bioRxiv - Cancer Biology 2023Quote: ... and (6) E-cadherin (1:500, 3195; Cell Signaling)–Opal 690.
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bioRxiv - Cancer Biology 2023Quote: ... and (6) E-cadherin (1:500, 3195; Cell Signaling)–Opal 650.
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bioRxiv - Pathology 2022Quote: ... (Cell Signaling 15019S, 1:5) and then with diluted anti-αSMA-Cy3 antibody (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated overnight at 4°C with primary antibodies diluted in 5% goat serum/ PBS: rabbit anti-PAX6 (1:250, 60433S, Cell Signaling), rabbit anti-SOX1 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked for 1 h at room temperature with 5% skim milk and then incubated overnight at 4°C with appropriate primary antibody dilution: anti-STAT1 (1:1000, Cell Signaling), anti-pSTAT1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1h with 5% BSA in TBS-T followed by overnight incubation at 4°C with antibody (Drosha 1:1000; D28B1, Cell Signaling, in 5 %BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were blocked with 3% BSA in PBS overnight at 4°C and then incubated with a rabbit phospho-SMAD1/5/9 antibody at 1:400 (D5B10, Cell Signaling) and a mouse anti-YAP antibody at 1:200 (63.7 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-pS6 (S235/6) 1:1000 (Cell Signaling #4858), rabbit anti-p-eIF2α S51 1:300 (Cell Signaling #9721) ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: ... rabbit polyclonal caspase-6 (Cell Signaling, 9762, WB 1:1000), mouse monoclonal anti-β-actin (Proteintech ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were probed overnight at 4 °C with antibodies against the following epitopes: phospho(Ser217/221)-MEK1/2 (1:1000; 41G9; Cell Signaling), MEK1/2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... containing 5% BSA for one hour at RT and incubated overnight at 4°C with anti-flotillin-2 (C42A3) (1:500, #3436, Cell Signaling), anti-calnexin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were fixed with 4% paraformaldehyde for 5 minutes and blocked in 5% goat normal serum (Cell Signaling Technologies, 5425) for an hour before antibody detection using antibodies listed in Supplementary Tables S5-S6 ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... pSMAD1/5 (1:400, Cell Signaling), mCherry (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... LAMP-2 (1:1 000, #49067, Cell Signaling), LC3Bxp (1:1 000 ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T, #9102, Cell Signaling) and phospho-p44/42 MAPK (Thr202/Tyr204 ...
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bioRxiv - Biochemistry 2020Quote: ... or glucose 6-phosphate dehydrogenase (8866, Cell Signaling Technology, 1:1000) antibodies and quantified as detailed above.
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated rotating for 2 hr at 4°C with 1 uL anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology) or normal rabbit IgG (#2729 ...