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• Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia

  Yoshinobu Ichimura, et al., bioRxiv - Cell Biology 2024

  Quote: ... TFEB (4240; Cell Signaling Technology, Danvers, MA, USA; 1:500), Cathepsin B (219361 ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cathepsin D (E179)(Cell Signaling, 69854S, Rabbit mAb), Cathepsin L (Santa Cruz Biotechnology ...
• Mitochondrial inner membrane remodeling as a driving force of organelle shaping

  Noga Preminger, et al., bioRxiv - Cell Biology 2024

  Quote: ... All knockdowns were verified by Western blotting using antibodies against Opa1 (67589, Cell Signaling Technology, USA), Mic10 (ab84969 ...
• GSK-3α-BNIP3 axis promotes mitophagy in human cardiomyocytes under hypoxia

  Hezlin Marzook, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cells were washed with PBS the next day and stained with secondary Alexa-fluor 488 antibody (Cell Signaling Technologies #4412S) for 1hr/RT and counterstained with DAPI ...
• Dysregulation in Spatiotemporal Expression of HMX1 Coordinated by Multifaceted Enhancers Drives an Auricular Disorder

  Xiaopeng Xu, et al., bioRxiv - Developmental Biology 2024

  Quote: ... TCF7L2 (Cell Signaling, catalog no. 2569S, 1 µg), and the mixture was incubated overnight on a nutator at 4°C ...
• Activation of Heme Metabolism Promotes Tissue Health After Intraarticular Injury or Surgical Exposure

  Suryamin Liman, et al., bioRxiv - Cell Biology 2024

  Quote: ... OPA-1 (Cell Signaling) were diluted 1:1000 in BSA 2.5% and incubated overnight at 4°C in a rotator shaker ...
• Critical role of Gα12 and Gα13 proteins in TGF-β-induced myofibroblast differentiation

  Eleanor B. Reed, et al., bioRxiv - Cell Biology 2024

  Quote: ... 1,000X) were from Cell Signaling Technology. Gα13 antibody (GTX32613 ...
• Activation of Heme Metabolism Promotes Tissue Health After Intraarticular Injury or Surgical Exposure

  Suryamin Liman, et al., bioRxiv - Cell Biology 2024

  Quote: ... HRP-linked secondary abs (Cell Signaling) diluted 1:2000 in BSA 2.5% were incubated by 1h RT ...
• Mitochondrial Calcium Signaling Regulates Branched-Chain Amino Acid Catabolism in Fibrolamellar Carcinoma

  Nicole M. Marsh, et al., bioRxiv - Cell Biology 2024

  Quote: ... human S292/mouse S293 antibody (Cell Signaling Technology, 40368); 1:1000 rabbit phospho-R-X-S*/T* (Cell Signaling Technology ...
• ATG5 is dispensable for ATG8ylation of cellular proteins

  Robin Ketteler, et al., bioRxiv - Cell Biology 2024

  Quote: ... LC3A/B (1:500, Cell Signaling Technology, no. 12741), SQSTM1 (1:1,000 ...
• ATG5 is dispensable for ATG8ylation of cellular proteins

  Robin Ketteler, et al., bioRxiv - Cell Biology 2024

  Quote: ... ATG5 (used to detect ATG12-ATG5 conjugate; 1:1000, Cell Signaling Technology, no. 2630), ATG7 (1:2,000 ...
• Large transient assemblies of Apaf1 constitute the apoptosome in cells

  Alicia C. Borgeaud, et al., bioRxiv - Cell Biology 2024

  Quote: ... except that the primary antibodies were mouse α-caspase-9 (full length and cleaved) (1:1000) (Cell Signaling, 9508) in 5% milk-PBS-Tween 20 and mouse α-PARP antibody (1:500 ...
• Prenylation controls proliferation in human pluripotent stem cell-derived cardiomyocytes

  Christopher A.P. Batho, et al., bioRxiv - Cell Biology 2024

  Quote: ... Ki-67 (1:400, 9129S, Cell Signaling Technology), HA-Tag (1:1000 ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit anti- Ikaros/IKZF1(Cell Signaling Technology), rabbit anti-GAPDH (Cell Signaling Technology).
• Clathrin-associated carriers enable recycling through a kiss-and-run mechanism

  Jiachao Xu, et al., bioRxiv - Cell Biology 2024

  Quote: ... and then fractionated by SDS–PAGE (Vazyme) and transferred to nitrocellulose membranes (Cell Signaling). The membranes were incubated in TBST buffer containing 5% skim milk for 1 h at room temperature ...
• ATG5 is dispensable for ATG8ylation of cellular proteins

  Robin Ketteler, et al., bioRxiv - Cell Biology 2024

  Quote: ... HRP-linked (1:5,000, Cell Signaling Technology, no. 7074/no. 7076); IRDye 800CW goat anti-rabbit/anti-mouse (1:15,000 with 0.02% SDS ...
• Intercellular adhesion boots collective cell migration through elevated membrane tension

  Brent M. Bijonowski, et al., bioRxiv - Cell Biology 2024

  Quote: ... the coverslips were incubated with 800:1 rabbit-anti-YAP antibody (#14074, Cell signaling), 200:1 rabbit-anti- pERM antibody (#3726 ...
• Intercellular adhesion boots collective cell migration through elevated membrane tension

  Brent M. Bijonowski, et al., bioRxiv - Cell Biology 2024

  Quote: ... 200:1 rabbit-anti- pERM antibody (#3726, Cell Signaling), 100:1 mouse-anti-Vinculin monoclonal antibody (13- 9777-82 ...
• Intercellular adhesion boots collective cell migration through elevated membrane tension

  Brent M. Bijonowski, et al., bioRxiv - Cell Biology 2024

  Quote: ... 1000:1 Alexa 488 anti-rabbit (#4412, Cell Signaling) or 1000:1 Alexa 488 anti-mouse (#A11029 ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cathepsin B (D1C7Y)(Cell Signaling, 31718S, Rabbit mAb), Cathepsin D (E179)(Cell Signaling ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: ... FAM134B (E8Y9R)(Cell Signaling, 83414S, Rabbit mAb), GAPDH (Proteintech ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: The following antibodies were used for western blotting and immunofluorescence studies: Alpha-actinin (D6F6)(Cell Signaling, 6487T, Rabbit mAb), BiP (Proteintech ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: ... LAMP1 (E6N3R)(Cell Signaling, 46843S, Rabbit mAb), LC3A/B (D3U4C)(Cell Signaling ...
• FICD deficiency protects mice from hypertrophy-induced heart failure via BiP-mediated activation of the UPR^ER and ER-phagy

  Shannon M. Lacy, et al., bioRxiv - Cell Biology 2024

  Quote: ... CHOP (D46F1)(Cell Signaling, 5554S, Rabbit mAb), Cathepsin B (D1C7Y)(Cell Signaling ...
• Dynamic and decay kinetics of H3 variants in live cells reveal the pivotal role of HIRA/NSD2 in maintaining the distinct H3.3 specific chromatin landscape

  Vishal Nehru, et al., bioRxiv - Cell Biology 2024

  Quote: ... and H3K36me3(9763) from Cell Signaling Technology. Horseradish peroxidase-conjugated secondary antibodies (ECL anti-rabbit IgG ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... HA-Tag (C29F4) Rabbit mAb (Cell Signaling Technology, 1:1000), HA-Tag (6E2 ...
• Mitochondrial Calcium Signaling Regulates Branched-Chain Amino Acid Catabolism in Fibrolamellar Carcinoma

  Nicole M. Marsh, et al., bioRxiv - Cell Biology 2024

  Quote: ... 1:10,000 HRP-linked α-mouse secondary antibody (Cell Signaling Technology, 7076).
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... HRP-linked Antibody (Cell Signaling Technology, 1:3500), Goat-anti-mouse IgG Light Chain HRP (1:5000) ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... at RT (Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology, 1:3500), Anti-mouse IgG ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... LC3B Antibody ((Cell Signaling Technology, 1:1000), Ubiquitin (P4D1 ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an anti-HA antibody (Cell Signaling Technology, 1:100). The next day ...
• Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity

  Irene Schwartz, et al., bioRxiv - Cell Biology 2024

  Quote: ... HA-Tag (6E2) Mouse mAb (Cell Signaling Technology, 1:1000), OLLAS Epitope Tag Antibody (L2 ...
• Endosomal actin branching, fission and receptor recycling require FCHSD2 recruitment by MICAL-L1

  Devin Frisby, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-acetylated tubulin (3971, Cell Signaling Technology, 1:300), anti-CP110 (12780 ...
• Endosomal actin branching, fission and receptor recycling require FCHSD2 recruitment by MICAL-L1

  Devin Frisby, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-EEA1 (3288, Cell Signaling, 1:30), anti-cortactin (05-180-I ...
• PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

  Koki Nakamura, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-β-actin (Cell Signaling Technology, 4967; 1:500), anti-FLAG (M2 ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit anti-GAPDH (Cell Signaling Technology).
• Prenylation controls proliferation in human pluripotent stem cell-derived cardiomyocytes

  Christopher A.P. Batho, et al., bioRxiv - Cell Biology 2024

  Quote: ... HA-Tag (1:1000, 3724S, Cell Signaling Technology) and/or pericentrin (1:2000 ...
• Regulation of the poly(A) Polymerase Star-PAP by a Nuclear Phosphoinositide Signalosome

  Tianmu Wen, et al., bioRxiv - Cell Biology 2024

  Quote: ... Post-PLA slides were further processed for immunofluorescent staining against the nuclear membrane marker (Lamin A/C, #8617, Cell Signaling). After washing three times with PBST ...
• ZIP13 regulates lipid metabolism by changing intracellular iron and zinc balance

  Ayako Fukunaka, et al., bioRxiv - Cell Biology 2024

  Quote: ... PKA (1:1000; Cell Signaling, #5842) and anti-GAPDH (1:3,000 ...
• A long-lived pool of PINK1 imparts a molecular memory of depolarisation-induced activity

  Liam Pollock, et al., bioRxiv - Cell Biology 2024

  Quote: Antibodies and other reagents are as follows: anti-PINK1 D8G3 (rabbit, 6946, 1:1000, Cell Signaling Technology), anti-pSer228-PINK1 (rabbit ...
• The role of ER exit sites in maintaining P-body organization and transmitting ER stress response during Drosophila melanogaster oogenesis

  Samantha N. Milano, et al., bioRxiv - Cell Biology 2024

  Quote: ... and Rabbit anti-Tri-methyl-Histone (C42D8) (1:150,000; Cell Signaling Technology). Bands visualized using TrueBlot ULTRA ...
• A long-lived pool of PINK1 imparts a molecular memory of depolarisation-induced activity

  Liam Pollock, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-pSer65-Ub E2J6T (rabbit, 62802, 1:1000, Cell Signaling Technology), anti-UBA1 (rabbit ...
• PTEN Regulates Myofibroblast Activation in Valvular Interstitials Cells based on Subcellular Localization

  Dilara Batan, et al., bioRxiv - Cell Biology 2024

  Quote: ... The sections were treated with the following primary antibodies (αSMA, mouse, Cat. No. ab7817, Abcam at 1:300 dilution, PTEN rabbit, Cat. No. D8H1X, Cell Signaling at 1:300 dilution) sequentially over 2 days with 2 overnight incubations with the primary antibody in blocking buffer (dilution 1:200 ...
• Formation of Multinucleated Osteoclasts Depends on an Oxidized Species of Cell Surface Associated La Protein

  Evgenia Leikina, et al., bioRxiv - Cell Biology 2024

  Quote: ... we incubated the cells with fluorescent secondary FAB fragments raised to the species corresponding to the primary antibody for 1 hr in PBS supplemented with 10% FBS (either Anti-rabbit IgG Fab2 Alexa Fluor ® 555 or Anti-mouse IgG Fab2 Alexa Flour ® 488, both Cell Signaling Technology, Catalogue # 647 4414 S and # 4408 S ...
• A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast

  Xiaoxue Zhou, et al., bioRxiv - Cell Biology 2024

  Quote: ... DyLight 800 conjugated secondary antibodies (Cell Signaling) were used at a 1 :10,000 dilution ...
• Loss of TLR9 expression in breast cancer tumour cells and its role in the cell cycle

  Emily Sible, et al., bioRxiv - Cell Biology 2024

  Quote: ... All the primary antibodies for western blotting were from Cell Signaling but the β actin (MP biomedicals ...
• Loss of the lysosomal protein CLN3 modifies the lipid content of the nuclear envelope leading to DNA damage and activation of YAP1 pro-apoptotic signaling

  Neuza Domingues, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-p73 (Cell Signaling Technology, 14620S; IB 1:1000, IF 1:100); anti-cAbl (Cell Signalling 2662P ...
• PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

  Koki Nakamura, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA-tag (Cell Signaling Technology, 3724; 1:200), anti-HA-tag (BioLegend ...
• PTEN Regulates Myofibroblast Activation in Valvular Interstitials Cells based on Subcellular Localization

  Dilara Batan, et al., bioRxiv - Cell Biology 2024

  Quote: ... PTEN (rabbit, Cat. No. D8H1X, Cell Signaling) at 1:300 dilution ...