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Citations for Jena Bioscience :
1 - 50 of 133 citations for Tubulin inhibitor 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Cell Biology 2020Quote: ... from a mixture of unlabeled and HiLyte647-labeled tubulin (5:1, total tubulin concentration 100 μM) and 1 mM GMPCPP (Jena Bioscience, Jena, Germany) incubated at 37°C for 20 min ...
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bioRxiv - Biophysics 2021Quote: ... MTs were prepared either with 0.75 µM mung tubulin or 30 µM goat brain tubulin in BRB-80 buffer with 1 mM GMPCPP (Jena Bioscience, Germany) and incubated at 37°C for 60 min ...
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bioRxiv - Molecular Biology 2019Quote: ... GMPCPP-stabilized seeds were made by two rounds of polymerization in 25 µM tubulin (40% dig-tubulin) supplemented with 1 mM GMPCPP (Jena Biosciences) as described (Volkov et al. ...
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bioRxiv - Cell Biology 2022Quote: ... labeled and biotin labeled tubulin 1 mM GMPCPP (Jena Bioscience, # NU405S) followed by incubation on ice for 10 min to allow nucleotide exchange and then polymerizing on the 37 °C water bath for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Microtubule seeds were prepared at 10 µM tubulin concentration (20 % ATTO-647-labelled tubulin and 80 % biotinylated tubulin) in BRB80 supplemented with 0.5 mM GMPCPP (Jena Bioscience) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2022Quote: Microtubule seeds were prepared at a final concentration of 10 µM tubulin (20 % ATTO-647-labelled tubulin and 80 % biotinylated tubulin) in BRB80 supplemented with 0.5 mM GMPCPP (Jena Bioscience) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... fluorescently labelled and non-labelled tubulin in a ratio 18:12:70 (total of 20 μM tubulin) with 1 mM GMPCPP (NU-405 Jena BioScience, Jena, Germany) at 37°C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... The tubulin mix (20 μM) with 1 mM GMPCPP (NU-405L; Jena Bioscience) in BRB80 (80 mM Pipes pH 6.9 ...
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bioRxiv - Cell Biology 2019Quote: ... at a total tubulin concentration of 20 µM with 1 mM GMPCPP (Jena Biosciences) at 37° C for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... at a total final tubulin concentration of 20 µM with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Tubulin solutions were mixed with 1 µl 10 mM GMP-CPP (#NU-405S, Jena Bioscience) on ice and incubated for 10 minutes to allow nucleotide exchange ...
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bioRxiv - Biophysics 2021Quote: ... Double cycle GMPCPP polymerisation was performed as follows: reconstituted tubulin was supplemented with 1 mM GMPCPP (Jena Biosciences) and incubated for 5 mins on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 68% unlabelled tubulin) for 1 h at 37 ֯C in BRB80 supplemented with 2.7 mM GMPCPP (Jena Bioscience, NU-405). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2021Quote: ... labeled:unlabeled tubulin (Hyman, 1991) in the presence of GMPCPP (Jena Biosciences) in two cycles ...
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bioRxiv - Biophysics 2021Quote: ... GMPCPP seeds were prepared by polymerizing a 1:4 molar ratio of TAMRA labelled:unlabelled tubulin in the presence of guanosine-5’-[(α, β)-methyleno]triphosphate (GMPCPP, Jena Biosciences) in two cycles ...
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bioRxiv - Cell Biology 2022Quote: ... 7 μM of biotinylated tubulin and 0.5 mM GMPCPP (Jena Bioscience, NU-405S) in BRB80 buffer was incubated at 37°C for 40 min ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Doubly stabilized MTs were polymerized by incubating 5 µM tubulin with 0.5 mM GMPCPP (Jena BioScience, #NU-405L) for 20 min at 37°C ...
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Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endbioRxiv - Biophysics 2023Quote: ... and purified tubulin was subsequently subjected to two polymerization/depolymerization cycles using 0.1 mM GMPCPP (NU-405L; Jena Bioscience) instead of 1 mM GTP ...
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bioRxiv - Biophysics 2022Quote: ... Alexa-Fluor-488-labeled stabilized microtubules was prepared by polymerizing Alexa-Fluor-488-conjugated bovine tubulin in the presence of slowly hydrolysable GTP analog GMPCPP (Jena Bioscience) as previously described (Gell et al. ...
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bioRxiv - Cell Biology 2024Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37°C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (#NU-405, Jena Bioscience). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Biophysics 2024Quote: Stable MT nucleation seeds were prepared by incubat-ing 30 µM tubulin with 0.8 mM GMPCPP (NU-405L; Jena Bioscience, Germany) in BRB-80 buffer (80 mM PIPES ...
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bioRxiv - Biophysics 2021Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37 °C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (Jena Bioscience, NU-405). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2024Quote: ... inhibitors (as detailed above, 0.25 mM MnCl2, 33uM fluorescently labeled dATP (Jena Bioscience, NU-1611-Cy3), 33uM biotinylated dUTP (Jena Bioscience ...
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bioRxiv - Plant Biology 2022Quote: ... and protease inhibitor cocktail) and incubated with nucleotide-linked agarose beads (AMP and ATP affinity test kits, Jena Biosciences, Jena, Germany) overnight on the wheel at 4°C at a final concentration of 0.2% ß-DM ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with SiR-Tet (1–2 µM, 1 h; Spirochrome, Stein am Rhein, Switzerland) or TAMRA-Tet (2 µM, 1 h; Jena BioScience, Germany) and washed again with fresh medium (3 × quick wash and 3 × 30 min wash ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
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bioRxiv - Biophysics 2023Quote: ... and Wizard 1&2 (Jena Bioscience) screens were used at 16 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... AF488-Azide (#CLK-1275-1, Jena Bioscience) was used to test success of bead – DBCO coupling ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GMPPCP (NU-405S, Jena Bioscience) or 1 mM BeF4 (prepared by mixing 50 mM BeSO4 and NaF 100 mM to make 10 mM BeF3) ...
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bioRxiv - Microbiology 2021Quote: ... Labeling was achieved by using a 1:1 molar ratio of dCTP:Biotin-11-dCTP (NU-809-BIOX, Jena Bioscience) in the dNTPs used for the PCR (GoTaq DNA polymerase ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C in click reagents (1:1 DMSO/H2O with 20 μM picolyl-azide-sulfo-Cy5 (Jena Bioscience), 300 μM CuSO4 ...
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bioRxiv - Biochemistry 2020Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Cell Biology 2022Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and 50 µg mL-1 nourseothricin (Jena Bioscience).
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bioRxiv - Microbiology 2023Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
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bioRxiv - Cell Biology 2024Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The assay was carried out at 37°C and the reaction was started by addition of 1 mM ATP and 1 mM GTP (Jena Bioscience), and samples for the nucleotide measurement were taken after 15 min ...
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bioRxiv - Immunology 2023Quote: ... and first PCR reaction was performed on 1 µl of a 1 in 100 diluted freeze-thawed cell pellet using the SCRIPT HF RT-PCR Kit (Jena Bioscience), according to the manufacturer’s protocol ...