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Citations for Jena Bioscience :
1 - 50 of 161 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
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bioRxiv - Cancer Biology 2020Quote: ... Binding was measured between EDA-GTP-Cy5 (Jena Bioscience GmbH, Jena, Germany) and His-tagged labeled proteins in Buffer I ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.3 mM GTP (CTP, UTP, and GTP from Jena Bioscience), and 4.5 mM Thio-Phosphate-GMP (Genaxxon Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM Mg-GTP or Mg-GMPCPP (Jena Biosciences), and an oxygen scavenging system (80 μg/mL Catalase (Sigma C1345-1G) ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM KCl buffer containing 1 mM GTP (Jena Bioscience) and 1 mM MgCl2 and the released inorganic phosphate was measured using a malachite green-based colorimetric assay (Baykov et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... for GTP analog treated samples Non-hydrolyzable GTP Test Kit (Jena Bioscience #NK-102) containing 5 analogs – GTPαS ...
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bioRxiv - Bioengineering 2023Quote: ... RAS loaded with mant•GDP (RAS•mGDP) was obtained by mixing RAS with a 10x stochiometric excess of mGDP (Jena Bioscience) in a buffer containing an excess (20 mM ...
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bioRxiv - Microbiology 2020Quote: ... ATP and GTP (Jena Bioscience), were separated prior to sample analysis ...
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bioRxiv - Microbiology 2024Quote: ... and GTP (Jena Bioscience, Germany) using the Quattro-Premier XE instrument (c-di-GMP and pGpG ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biophysics 2023Quote: ... Ras densities were measured by loading Ras with Atto-488-GDP (Jena Bioscience) using SOSCat ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: ... and 1mM GTP (Jena Bioscience, NU-1012). The polymerized microtubules were diluted in BRB80T (BRB80 supplemented with 10μM taxol (paclitaxel) ...
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bioRxiv - Biophysics 2021Quote: ... and 1mM GTP (Jena Bioscience, NU-1012). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM GTP (Jena Bioscience, NU-1047), 250 μg/ml glucose oxidase (Serva ...
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bioRxiv - Cell Biology 2024Quote: ... and 1mM GTP (#NU-1012, Jena Bioscience). The polymerized microtubules were diluted in BRB80T and centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: ... RAS loaded with GppNHp was obtained by mixing RAS with a 10x stochiometric excess of GppNHp (Jena Bioscience) in a buffer containing an excess (20 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3’deoxy GTP (3′dGTP) from Jena Bioscience.
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bioRxiv - Molecular Biology 2022Quote: ... 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience), and 1 µl of RNase inhibitor (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... Biotin-11-GTP (100 μM; Jena Bioscience, NU-971-BIOX) and biotin-11-CTP (100 μM ...
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bioRxiv - Biophysics 2021Quote: The P3-[1-(2-nitrophenyl)ethyl] ester (NPE) of GTP was obtained from Jena Bioscience (Jena, Germany). P3-[para-hydroxyphenacyl] ester (pHP ...
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bioRxiv - Microbiology 2020Quote: ... The assay was carried out at 37°C and the reaction was started by addition of 1 mM ATP and 1 mM GTP (Jena Bioscience), and samples for the nucleotide measurement were taken after 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience) in BRB80 buffer and 2.5 mM MgCl2 for 35 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl and time-lapse images were acquired while flowing in 0.3 μM dynamin mixed with 1 mM GTP (Jena Bioscience) and 1 mM MgCl2 in 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.
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bioRxiv - Biophysics 2021Quote: Tubulin polymerization kinetics were measured for concentrations ranging betwee 0.25 µM and 2 µM were polymerized by incubation with BRB-80 buffer containing 10% glycerol and either 1 mM GTP (Jena Bioscience, Germany) with 1 mM MgCl2 or 10 µM taxol ...
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bioRxiv - Biochemistry 2023Quote: ... and GTP or GDPCP were assembled in 1×10 buffer with 10 mM βME by preincubation of eEF1A and GTP (PromegaTM Corp., Madison, WI) or GDPCP (Jena Bioscience, Jena, Germany) for 5 minutes at 37°C followed by addition of tRNAAla or Ala-tRNAAla for 1 minute at 37°C and were then kept on ice until use ...
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bioRxiv - Biophysics 2021Quote: ... GTP (NU-1012) and GppNHp - Tetralithium salt (GMPPNP) (NU-401) were purchased from Jena Biosciences. BL21-CodonPlus and Terrific Broth were purchased from Agilent Technologies and Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 % of UTP (resp. GTP) was substituted with either 5-ethynyl-UTP (5EU, Jena Bioscience), 5-bromo-UTP (5BrU ...
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bioRxiv - Molecular Biology 2019Quote: ... for unlabelled samples or 15N-labelled UTP and GTP (Eurisotop) and unlabelled ATP and CTP (Jena Bioscience) for 15N-[U/G]-labelled samples ...
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bioRxiv - Cell Biology 2019Quote: ... were generated by polymerization in the presence of the non-hydrolysable GTP analogue GMPCPP (NU-405S, Jena Bioscience) and then immobilized on coverslips incubated sequentially with the following solutions ...
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bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Alexa-Fluor-488-labeled stabilized microtubules was prepared by polymerizing Alexa-Fluor-488-conjugated bovine tubulin in the presence of slowly hydrolysable GTP analog GMPCPP (Jena Bioscience) as previously described (Gell et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience, Germany). After confirmation of GDP degradation by HPLC ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolysable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Biophysics 2019Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolyzable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Microbiology 2020Quote: ... ATP binding assays were performed according to the manufacturer’s protocol (Jena Biosciences). Here we used 6AH-ATP-Agarose (N6 - (6-Amino ...
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bioRxiv - Cell Biology 2020Quote: ... The incorporated EdU was visualized by click-it chemistry-mediated binding of Biotin (Azide-PEG3-Biotin Conjugate; Jena Biosciences) using the protocol and reagents from the Invitrogen Click-iT EdU Cell Proliferation Kit for Imaging (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Nucleotide release and binding measurements were performed using the fluorescent nucleotide analogues MABA-ADP (Jena Bioscience, NU-893-MNT) and MABA-ATP (Jena Bioscience ...
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bioRxiv - Cell Biology 2022Quote: Nucleotide binding assays were carried out by measuring the incorporation of 2,3-0-N-Methyl-anthraniloyl (Mant)-labelled nucleotides (Jena Bioscience) using an Synergy2 plate reader (BioTek Instruments Inc. ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were incubated with a 1:500 dilution of an anti-dsRNA J2 antibody (Jena Bioscience) in PBS supplemented with 1% FCS at 4°C overnight with shaking ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous and overexpressed PML was immunofluorescently labelled with anti-PML antibody (rabbit, ABD-030, Jena Bioscience, Germany, 1:500), followed by secondary antibody coupled with STAR 580 STED dye (goat-anti-rabbit ...
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bioRxiv - Biochemistry 2020Quote: ... Additives as indicated were added during the primary antibody incubation step at a final concentration of 1 µM for adenosine (Jena Bioscience), AMP (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... anti-dsRNA monoclonal antibody (Jena Bioscience, Germany ...
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bioRxiv - Microbiology 2021Quote: ... The mouse anti-dsRNA antibody (J2) was from Jena Bioscience and rabbit anti-Calnexin from Elabscience ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...